Hostname: page-component-848d4c4894-5nwft Total loading time: 0 Render date: 2024-05-07T19:30:07.728Z Has data issue: false hasContentIssue false
  • English
  • Français

A Spot Test for Estimating Ureides in Soyabean Petioles

Published online by Cambridge University Press:  03 October 2008

Richard A. Glenister  and
Thomas A. LaRue
Richard A. Glenister
Affiliation:
Boyce Thompson Institute for Plant Research, Ithaca, New York 14853, USA
Thomas A. LaRue
Affiliation:
Boyce Thompson Institute for Plant Research, Ithaca, New York 14853, USA
Get access Rights & Permissions [Opens in a new window]

Summary

The ureides allantoic acid and allantoin form a bright pink compound with acidic p-dimethylamino cinnamaldehyde. It is possible to estimate the ureide content of a soyabean petiole segment by crushing it on filter paper and developing the colour with this reagent. There is a strong correlation between the spot test and estimates from colorimetric determination of ureides. In field grown soyabeans, there was a positive correlation between the petiole ureide content during pod fill and the integrated seasonal acetylene reduction activity. The spot test can be used to identify plants with low nitrogen fixation activity.

Type
Research Article
Information
Experimental Agriculture , Volume 22 , Issue 4 , October 1986 , pp. 405 - 416
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Hermanowicz, W. (1948). Formation of allantoic acid from allantoin. Rocznike Chem. 22:159–180 (from Chem. Abstracts 44:1418h).Google Scholar
Herridge, D. F. (1982). Use of the ureide technique to describe the nitrogen economy of field-grown soybeans. Plant Physiology 70:7–11.CrossRefGoogle ScholarPubMed
Herridge, D. F. & Pate, J. S. (1977). Utilization of net photosynthesis for nitrogen fixation and protein production in an annual legume. Plant Physiology 60:759–764.CrossRefGoogle Scholar
McClure, P. R., Israel, D. W. & Volk, R. J. (1980). Evaluation of the relative ureide content of xylem sap as an indication of nitrogen fixation in soybeans. Plant Physiology 66:720–725.CrossRefGoogle Scholar
McNeil, D. L. & LaRue, T. A. (1984). Effect of nitrogen source on ureides in soybean. Plant Physiology 74:227–232CrossRefGoogle ScholarPubMed
Patterson, T. G., Glenister, R. & LaRue, T. A. (1982). Simple estimate of ureide in soybean tissue. Analytical Biochemistry 119:90–95.CrossRefGoogle ScholarPubMed
Patterson, T. G. & LaRue, T. A. (1983a). Nitrogen fixation by soybeans: seasonal and cultivar effects and comparison of estimates. Crop Science 23:488–492.CrossRefGoogle Scholar
Patterson, T. G. & LaRue, T. A. (1983b). Dinitrogen fixation (C2H2) and ureide content of soybeans. Environmental effects and source-sink manipulation. Crop Science 23:818–824.CrossRefGoogle Scholar
Patterson, T. G. & LaRue, T. A. (1983c). N2 fixation (C2H2) and ureide content of soybean: Ureides as an index of fixation. Crop Science 23:825–831.CrossRefGoogle Scholar
Prasad, M. & Spiers, T. M. (1984). Evaluation of a rapid method for plant sap nitrate analysis. Communications in Soil Science and Plant Analysis 15:673–679.CrossRefGoogle Scholar
Van Berkum, P., Sloger, C., Weber, D. G., Cregan, P. B. & Keyser, H. (1985). Relationship between ureide N and N2 fixation, above ground N accumulation, acetylene reduction and nodule mass in greenhouse and field studies with Glycine max L. (Merr.) Plant Physiology 73:511–513.CrossRefGoogle Scholar
Vogels, G. D. & van der Drift, C. (1970). Differential analysis of glyoxylate derivatives. Analytical Biochemistry 33:143–157.CrossRefGoogle ScholarPubMed