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EFFECT OF SOIL DRYING ON RATE OF STRESS DEVELOPMENT, LEAF GAS EXCHANGE AND PROLINE ACCUMULATION IN ROBUSTA COFFEE (COFFEA CANEPHORA PIERRE EX FROEHNER) CLONES

Published online by Cambridge University Press:  02 December 2013


S. G. TESFAYE
Affiliation:
Jima Agricultural Research Center, Ethiopian Institute of Agricultural Research, P.O. Box 192, Jima, Ethiopia
M. R. ISMAIL
Affiliation:
Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
M. F. RAMLAN
Affiliation:
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
M. MARZIAH
Affiliation:
Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
H. KAUSAR
Affiliation:
Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Corresponding
E-mail address:

Summary

Seasonal drought stresses as a result of changes in global climate and local weather conditions are among the major factors adversely affecting growth and productivity of Robusta coffee (Coffea canephora pierre ex froehner) in many areas producing the crop. It is believed that there exists a wide range of genetic variability among Robusta coffee clones for traits associated with drought tolerance. Therefore, in an attempt to determine differences among Robusta coffee clones for some growth, physiological and biochemical parameters and identify drought-tolerant materials, 12 months old seedlings of six clones (IC-2, IC-3, IC-4, IC-6, IC-8 and R-4) were subjected to two treatments: well-watered control and drought-stressed (soil drying) by withholding irrigation for three weeks in a rain shelter at University Putra Malaysia, Malaysia. The rate of stress development, expressed as extent of wilting and damage to leaves, was considerably higher for clones IC-8, IC-4, R-4 and IC-2 than for IC-3 and IC-6 during the stress period. Leaf water potential (LWP), stomatal conductance (gs) and rate of net photosynthesis (PN) progressively decreased but leaf proline (LP) concentration substantially increased with time of exposure of the plants to soil drying. There was a considerable difference between coffee clones for the rate of change in these parameters. All the clones except IC-6 and IC-8 showed a negative carbon balance with the most negative value for R-4 at the end of the stress period. Six days after rewatering, LWP, gs and PN increased rapidly while LP concentration decreased and reached a level equivalent to those of well-watered plants, especially for clones IC-3 and IC-6. Among the drought-stressed plants, IC-6 and IC-3 had still significantly higher shoot growth, total dry matter yield and root to shoot ratio than did IC-2, IC-4, IC-8 and R-4. In general, clones IC-6 and IC-3 exhibited quite a better performance in almost all the parameters considered in this study and seemed to be less sensitive to drought stress. Hence, drought tolerance attributes in these clones could be linked to a more effective osmotic adjustment due to more rapid accumulation of LP and probably some morphological parameters, such as increased root-to-shoot ratio. However, further analyses at molecular level, detail biochemical studies and observations under diverse field conditions are required to come up with more conclusive recommendations.


Type
Research Article
Creative Commons
The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence http://creativecommons.org/licenses/by/3.0/
Copyright
Copyright © Cambridge University Press 2013

INTRODUCTION

Coffee is one of the most important commodities in the world market and it plays a significant role in the national economy of many developing countries. Among the economically important species of the genus Coffea, Robusta coffee (C. canephora Pierre ex Froehner) accounts for about 20% of the total coffee trade in the world market (Willson, Reference Willson1999). However, its production is greatly affected by frequent drought stress, specially in areas with long dry spells. It is believed that there exists a wide range of genetic variability among Robusta coffee clones for morphological, physiological and biochemical traits associated with drought tolerance (Anim-Kwapong et al., Reference Anim-Kwapong, Anim-Kwapong and Adomako2011; Lima et al., Reference Lima, DaMatta, Pinheiro, Totola and Loureiro2002). Besides morphological stress avoidance or tolerance mechanisms, such as decline in shoot growth and leaf area and increase in leaf thickness and root to shoot ratio with soil moisture depletion (Abernethy and McManus, Reference Abernethy and McManus1998; Rhizopoulou et al., Reference Rhizopoulou, Meletiou-Christou and Diamantoglou1991; Volkmar and Woodbury, Reference Volkmar, Woodbury and Pessarakli1995), stomatal closure and the rate of decline in leaf water potential (LWP) and net photosynthesis (PN) (Price et al., Reference Price, Jalaluddin, Dilday and Kuo1992) are also regarded as sensitive physiological indicators of the ability of a genotype to adapt stress conditions (Loewenstein and Pallardy, Reference Loewenstein and Pallardy1998a, Reference Loewenstein and Pallardyb; Saliendra et al., Reference Saliendra, Sperry and Comstock1995).

On the other hand, it has been reported that leaf scorching (damage to leaves due to water deficit stress) was inversely related to shoot growth parameters and can be used as an important attribute to screen Robusta coffee clones for drought tolerance (Anim-Kwapong et al., Reference Anim-Kwapong, Anim-Kwapong and Adomako2011). Because of its role in osmotic adjustment (OA), the accumulation of proline in plant tissues under water deficit condition has also been considered as one of the adaptive mechanisms in drought-tolerant genotypes (Heuer, Reference Heuer and Pessarakli1999; Ismail et al., Reference Ismail, Aziz and Hashim1994; Maestri et al., Reference Maestri, Da Matta, Regazzi and Barros1995). In fact, Lima et al. (Reference Lima, DaMatta, Pinheiro, Totola and Loureiro2002) and Anim-Kwapong et al. (Reference Anim-Kwapong, Anim-Kwapong and Adomako2011) have reported the existence of large diversity among Robusta coffee genotypes around the world for drought tolerance, although these variabilities have not been well studied and documented in relation to physiological and biochemical traits.

The major objective of this study was, therefore, to determine differences between six widely grown Robusta coffee clones for some morphological, physiological and biochemical parameters associated with drought tolerance and to identify the most important mechanisms involved in the process of tolerance.

MATERIALS AND METHODS

Plant material

Six clones of C. canephora Pierre ex Froehner (clones IC-2, IC-3, IC-4, IC-6, IC-8 and R-4), which are widely grown by local coffee farmers in the Northern part of Malaysia, were used in this experiment. The plants were grown in pots (with a diameter of 20 cm and a height of 25 cm) filled with a mixture of top soil (Serdang series), manure and sand (3:2:2 ratio by volume), irrigated daily and fertilized with 20-g NPK per pot during establishment. When the plants were 12 months old, drought stress was imposed by withholding irrigation until severe wilting symptoms were observed.

Treatment and plot arrangement

Seventy-two uniformly grown seedlings were selected from each clone and arranged in six plots each consisting of 12 plants (three well-watered (control) and three drought-stressed plots). Each drought-stressed plot was further sub-divided into two plots, each with six plants. One-half of the plot was rewatered when the seedlings showed severe wilting symptoms just 12 days after withholding irrigation and the rate of recovery in LWP, gs and PN, and changes in leaf proline (LP) concentration were measured. The other half of the plot was further maintained under drought stress condition for nine days to see changes in the degree of leaf folding or wilting, rate of leaf fall, loss of total leaf area and total dry matter yield and proportion of plants recovering upon rewatering after 21 days of soil drying, while the plants in the control plots were watered every other day throughout the experiment period. Besides, plant height, main stem diameter (girth), total leaf area, total dry matter yield and root to shoot ratio were also measured in the second set of experiment just after 21 days of soil drying. On the other hand, 18 uniformly grown seedlings were selected from well-watered plots of each clone, arranged as six plants per plot and planted out in larger pots (with 100-cm diameter and 100-cm height, open at both upper and bottom sides and filled with top soil (Serdang series) from the field) and maintained under rain-fed field condition to see their growth performance (plant height, girth and canopy diameter) and sensitivity to dry spells.

Study design and site description

The experiment was carried out in a randomized complete block design (RCBD) with three replicates of 12 factorial treatment combinations (two water regimes × six clones) in a rain shelter with average midday photosynthetic photon flux density (PPFD) of 672.3 μmol m−2 s−1 and air temperature and relative humidity (RH) of 34.5 °C and 62.9%, respectively. The rain shelter was located at the Hydroponics Unit of Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia (3°02ʹ N; 101°42ʹ E and 31 m above sea level). The open field observation under rain-fed condition was also carried out in RCBD with three replications of six clones. The mean maximum and minimum temperatures at UPM during the study period were 32.4 °C and 22.8 °C, respectively, with mean relative humidity of 65.5%.

Sampling procedure and data collection

Leaf water potential, stomatal conductance (gs), PN and LP concentration were measured on fully expanded leaves on the third and fourth nodes from the apex of plageotropic branches. On each sampling date, measurements were taken during noon hours (between 1100 and 1300 hours) under clear sky conditions during the stress period and after rewatering.

Leaf gas exchange

Rate of net PN was measured at four-day intervals, using a portable infrared gas analyzer (Port. Li-6200 Photosynthesis System, Licor, Lincoln, Nebraska, USA). LWP and gs were measured every three days on similar leaf samples as used for PN. LWP was determined with a pressure chamber (PMS, Soil Moisture Equipment, Santa Barbara, USA), while gs was measured using diffusive porometer (AP-4, Delta T Devices Ltd., Cambridge, UK).

Proline assay

The accumulation of free proline in coffee leaves was determined at three-day intervals following the procedure described by Bates et al. (Reference Bates, Waldren and Teare1973). Fully expanded leaves (same as those used for measuring LWP, gs and PN) were excised and used for the determination of LP concentration. LP content was measured using 0.5-g sample of fresh leaves. The samples were homogenized in 10 ml of 3% aqueous sulfosalicylic acid and filtered through Whatman No. 2 paper. Then 2 ml of filtrate was mixed with 2 ml of acid-ninhydrin and 2 ml of glacial acetic acid and heated at 100 °C for 1 h under water bath condition. Then the reaction was terminated in an ice bath for 10 min; then 4 ml of toluene was added to the mixture and contents of test tubes were stirred for 15 to 30 s. The chromophore was aspirated from the aqueous phase, and the absorbance was read at 520 nm using toluene as a blank (Parimala and Muthuchelian, Reference Parimala and Muthuchelian2011; Siamak et al., Reference Siamak, Sariah, Zakaria, Sreeramanan and Maziah2012).

Leaf folding and rate of leaf fall

The rate of drought stress development in the plant, expressed as the extent of leaf folding or wilting and damage on leaves (leaf scorching) at noon hours (between 1200 and 1300 hours) and early in the morning (at 0600 hours before sun rise), was estimated by visual scoring at three-day intervals, with values ranging between 1 and 5 as described by Rosario et al. (Reference Rosario, Ocampo, Sumague, Paje and Kuo1992) and Anim-Kwapong et al. (Reference Anim-Kwapong, Anim-Kwapong and Adomako2011) (scored using 1 to 5 scale, where 1: indicating all leaves green and turgid, 2: most leaves still turgid except the youngest which show leaf folding at noon and recover during the night time, 3: all leaves wilt and/or show leaf folding and do not recover during the night time (symptoms of senescence evident), 4: leaves (specially the older ones) turning pale green, showing severe wilting or folding and partially dry and 5: leaves turning brown and dry, mostly drooping). Each plant in a drought-stressed plot was evaluated during visual rating and the sum of scores on individual plants was divided by the number of plants in the plot to estimate the mean values per plot in each replication. Besides, the proportion of plants which exhibited wilting symptoms was also calculated based on counts taken every three days, and the percentage of plants recovering was also calculated based on counts taken two weeks after the commencement of rewatering on day 21 for each drought-stressed plot. All the plants in drought-stressed plots were considered to determine rate of leaf fall at the end of the stress cycle and rate of recovery (initiation of new flushes) upon rewatering after 21 days of withholding irrigation. The extent of leaf shedding or rate of leaf fall was calculated as the proportion of dried and fallen leaves from the total number of leaves produced by the plant.

Vegetative growth parameters and total dry matter yield

Plant height, girth (stem diameter at the base of the main stem), total leaf area and total dry matter yield were measured using two randomly selected seedlings from each plot at the end of the experiment. Total green leaf area of sampled plants was measured using an automatic leaf area meter (Delta T Device, Cambridge, UK). Loss of total leaf area and total dry matter production due to soil drying was estimated based on the respective green leaf area and total dry matter yield of plants in drought-stressed and well-watered plots just 21 days after withholding irrigation. Plants sampled from each plot were separated into shoot part (leaves, stem and branches) and roots and dried in an oven at 80 °C to a constant weight. Then each plant part was weighed to determine total dry matter yield and root to shoot ratio at the end of the stress period.

On the other hand, growth parameters for the field observation were measured using three randomly selected plants from each plot. Plant height (from soil surface to tip of the main stem), girth (main stem diameter at 10 cm height from the soil surface) and canopy diameter (from the tip of the longest primary branch at one side to the tip of the longest primary branch at the opposite side of the shoot) were measured 18 months after transplanting, while mean sensitivity to drought was scored using visual rating (1–5 scale, as described above) on all the plants in each plot at peak dry spells during this period.

Statistical analysis

All the variables measured in the course of the study were analysed using the SAS statistical software (SAS Institute Inc., 1988).

RESULTS

Leaf water potential

Leaf water potential progressively decreased from less than –1.0 to –3.4 MPa with time of exposure of coffee plants to soil drying. The rate of decline in LWP was much higher and faster at later stages (after six days) than at the early stages of stress development. There was also a considerable difference between coffee clones for LWP. The rate of decline in LWP was lower in clone IC-2, IC-4, IC-8 and R-4 than in IC-3 and IC-6 just three days after withholding irrigation. Six days after withholding irrigation, the rate of reduction in LWP was still lower in IC-2, IC-4, IC-8 and R-4 than in IC-3 and IC-6, where it was decreased by about 75% compared with the controls. In general, LWP decreased to the lowest value for all the clones at the end of the stress period on day 12 (Table 1). On the other hand, clones IC-8, IC-6 and IC-2 had considerably higher LWP under well-watered condition (Figure 1).

Table 1. Leaf water potential (LWP), stomatal conductance (gs), rate of net photosynthesis (PN), leaf proline concentration (LPC), sensitivity as estimated by visual stress score values (SSV) and proportion of plants showing complete wilting symptoms (PPCWS) on day 12 of soil drying.

Figure 1. Leaf water potential (LWP) of six Robusta coffee clones as affected by water stress and its recovery upon rewatering 12 days after soil drying (closed symbols represent water-stressed and open ones well-watered treatments). Bars represent standard error of means of each three observations.

After rewatering, LWP of IC-2, IC-3, IC-4 and IC-6 rapidly increased within three days and it reached a level equivalent to that of well-watered plants (>–1.0 MPa), especially for IC-3 and IC-6 just six days after rewatering. In general, IC-3 and IC-6 showed significantly faster rate of recovery than did other clones upon rewatering at the end of the soil drying cycle. On the other hand, IC-8 and R-4 exhibited a slower rate of recovery and significantly lower values of LWP than did the other clones on day 3 and even six days after rewatering (Figure 1).

Stomatal conductance

Stomatal conductance of all coffee clones was substantially decreased as the intensity of water deficit stress increased with time of soil drying. Differences among the clones were also considerable at the initial stage (under well-watered condition) and on day 3 and 6 after the plants were subjected to soil drying. The rate of decline in gs was faster for clones IC-2, IC-4, IC-8 and R-4 (61–71%) than for clones IC-3 and IC-6 (only 27–28%) during the early stages of the stress period until day 3. In general, gs of clones IC-3 and IC-6 decreased gradually at a relatively constant rate until day 9, and it was maintained at considerably higher level on day 3, compared with its mean values on the same day for the other clones. However, all the clones had a similar and the lowest value of gs on day 12 after withholding irrigation, where the stomata were nearly closed (Figure 2, Table 1).

Figure 2. Stomatal conductance (gs) of six Robusta coffee clones as affected by soil drying and rate of recovery upon rewatering 12 days after water deficit stress (open symbols represent well-watered control plants and closed symbols represent water-stressed ones). Bars represent standard error of means of each three observations.

There was also a considerable difference between coffee clones for gs after rewatering at the end of the soil drying period on day 12. In general, it was observed that clones IC-3 and IC-6 maintained significantly higher level of gs at a faster rate (on day 3) than did clones IC-2 and IC-4, which showed gradual recovery in gs within six days after rewatering. On the other hand, gs values of IC-8 and R-4 were not much improved by rewatering and was significantly lower than that of the other clones even on day 6 after rewatering. The gs values for drought-stressed plants of clones IC-3 and IC-6 were similar to those of the well-watered controls, but were slightly lower than the control values for clones IC-2 and IC-4 just six days after rewatering (Figure 2).

Rate of photosynthesis

There were considerable differences between coffee clones for the rate of PN under both well-watered and drought-stressed conditions. Clone IC-6, followed by IC-8, had significantly higher rate of net PN than did IC-2, IC-3, IC-4 and R-4 at the early stages of stress on day 4 after exposure of plants to soil drying. In general, water-deficit stress reduced the rate of net PN by about 68% compared with the well-watered plants during the first week of the stress period. The decline in PN due to drought stress was considerably higher and more obvious on days 8 and 12 than on day 4 in IC-6 and IC-8 (Figure 3).

Figure 3. Rate of net photosynthesis (PN) of six Robusta coffee clones as affected by soil drying and rewatering on day 12 when complete wilting symptoms were observed (open symbols represent well-watered and closed ones water-stressed treatments). Bars represent standard error of means of each three observations.

All the clones except IC-6 and IC-8 showed a negative carbon balance with more negative value for clone R-4 at the end of the stress period on day 12 (Table 1). On the other hand, the rate of recovery in net PN was considerably higher in IC-6, followed by IC-3, than for IC-2, IC-4, IC-8 and R-4 just four days after rewatering. Eight days after the commencement of rewatering, PN rate in IC-3 and IC-6 reached the level of the control plants, but it was still lower than that of the controls in IC-2, IC-4, IC-8 and R-4, with the lowest value being for clone R-4 (Figure 3).

Proline accumulation in leaves

All coffee clones responded to soil drying by substantial increase in their LP concentration by as much as 14 folds compared with the controls. The control plots also exhibited variations in proline accumulation. Three days after withholding irrigation, the accumulation of LP was highest (over 14 folds) in IC-6, but it increased by only 84% in IC-4 and the remaining four clones exhibited 112 to 157% increase over their respective controls. The level of LP reached its peak in all the clones 12 days after withholding irrigation (Table 1), although there was only a slight increment from values measured on day 9 for IC-2, IC-3, IC-6 and R-4 and it increased progressively in IC-4 and IC-8 (Figure 4). LP concentration of all the clones decreased when the plants were rewatered at the end of the soil drying cycle on day 12. However, the rate of its decline differed between the clones, as it dropped to the level exhibited by well-watered control plants in IC-3 and IC-6 but was still higher in stressed plants than in non-stressed controls, especially in IC-8 and R-4 (Figure 4).

Figure 4. Leaf proline concentration (LPC) of six Robusta coffee clones as affected by soil drying and rewatering on day 12 when complete wilting symptoms were observed (open symbols represent well-watered and closed ones water-stressed treatments). Bars represent standard error of means of each three observations.

Leaf folding and rate of leaf fall

The rate of leaf folding or extent of wilting was considerably higher for clones IC-8, IC-4, R-4 and IC-2 than for IC-3 and IC-6 during the stress period. Clones IC-8, R-4, IC-2 and IC-4 exhibited severe wilting symptom with necrosis or chlorosis lesions on leaves 15 days after withholding irrigation. The stressed plants of especially these clones progressively showed a permanent wilting symptom and severe leaf drying or scorching and leaf fall 18 days after the commencement of the soil drying treatment (Figure 5). Clone IC-6, followed by IC-3, also showed significantly lower stress score values than did the rest of the clones during the dry spells under field condition (Table 4). However, sensitivity of all the clones to drought was higher in the rain shelter than under field condition (Tables 2 and 4).

Table 2. Sensitivity of Robusta coffee clones to soil drying as estimated by stress score values (SSV), rate of leaf fall (RLF), loss of total leaf area (LTLA) and total dry matter yield (LTDMY) and percentage of plants recovering (PPR) upon rewatering on day 21 after soil drying.

Figures followed by the same letters within a column are not significantly different at p = 0.05.

Figure 5. Sensitivity of Robusta coffee clones to soil drying as estimated by stress scores (visual rating of the extent of wilting or leaf folding and damage to leaves). Bars represent standard error of means of each three observations.

The proportion of plants which showed the first wilting symptom (partial leaf folding) was higher (33%) for IC-4 and IC-8 compared with IC-2, IC-3, IC-6 and R-4, 13 to 23% of plants of which exhibited the symptom three days after soil drying. On the other hand, 53 to 60% of the plants of all the clones except IC-3 and IC-6 showed obvious wilting symptoms six days after the stress treatment. Mean percentage of plants wilted increased to 90% for IC-2 and IC-4 and to 87% for IC-8 and R-4, but it was 70–77% for IC-3 and IC-6 after nine days of withholding water. Three days later, about 97% of the plants of IC-2, IC-4, IC-8 and R-4 wilted completely, while about 87% of those of IC-3 and IC-6 showed such a complete wilting symptom (Figure 6, Table 1). As a result, rate of leaf fall (51.52–65.05%) and loss of total leaf area (49.01–57.79%) were significantly lower, but percentage of plants recovered upon rewatering after 21 days of drought stress was considerably higher (86.5%) for IC-6 and IC-3 than for IC-2, IC-4, IC-8 and R-4, for which the respective values ranged from 78.73 to 90.89%, 62.79 to 74.35% and 36.5 to 71.5% (Table 2).

Figure 6. Sensitivity of Robusta coffee clones to soil drying as estimated by proportion of plants showing complete wilting symptoms. Bars represent standard error of means of each three observations.

Vegetative growth and total dry matter yield

Plant height, girth, total leaf area, total dry matter yield and root to shoot ratio were significantly affected by drought stress. There were also significant differences among the clones for these parameters. It was observed that clone IC-6, followed by IC-3, exhibited the highest plant height under well-watered condition. Among the drought-stressed plots, clones IC-6 and IC-3 had still higher plant height, total dry matter yield and root to shoot ratio than did the other clones. The highest girth and the total leaf area were also observed for clone IC-6 under both well-watered and drought-stressed conditions (Table 3).

Table 3. Growth and total dry matter yield (TDMY) of Robusta coffee clones under well-watered (WW) and drought-stressed (DS) conditions at seedling stage after 21 days of soil drying.

Figures followed by the same letters within a column are not significantly different at p = 0.05.

PHt. = plant height; TLA = total leaf area per plant.

Similarly, as observed in the rain shelter, plant height, girth and canopy diameter were significantly higher, but sensitivity to dry spells as estimated by visual rating for the rate of stress development was significantly lower for clone IC-6, followed by IC-3, than for IC-2, IC-4, IC-8 and R-4 under rain-fed field condition (Table 4).

Table 4. Growth and sensitivity of Robusta coffee clones to dry spells as estimated by stress score values (SSV: 1–5 scale) under field rain-fed condition.

Figures followed by the same letters within a column are not significantly different at p = 0.05.

PHt. = plant height; CD = canopy diameter.

DISCUSSION

Leaf gas exchange

Leaf water potential, gs and PN declined substantially with prolonged period of soil drying. Although the rate of decline varied with clone, these parameters had the lowest values for all the clones at the end of the stress period on day 12 (Figures 1–3, Table 1). These results were quite in agreement with the findings of Lima et al. (Reference Lima, DaMatta, Pinheiro, Totola and Loureiro2002) on pot-grown plants of both drought-sensitive and drought-tolerant Robusta coffee clones, where LWP has decreased to –3.0 MPa and led to complete suppression of gs and net PN six days after withholding irrigation. Similar results have also been reported for Arabica coffee, where gs and the rate of net PN began to decline as LWP fell below –2.0 MPa and became almost zero when LWP decreased to –3.0 MPa after 11 days of water stress (Kanechi et al., Reference Kanechi, Uchida, Yasuda and Yamaguchi1988). As observed in the present study, especially for coffee clones IC-3 and IC-6, it has been reported that drought stress usually causes significant decreases in both PN rate and gs (Krieg, Reference Krieg1983) as a consequence of considerable reduction in LWP of plants (Tardieu and Davies, Reference Tardieu and Davies1992). Such decreases in PN and gs with reductions in plant water status have been observed in drought-stressed plants of banana, peanut and rice (Adam and Barakbah, Reference Adam and Barakbah1990), tomato (Ismail and Dalia, Reference Ismail and Dalia1995), evergreen sclerophylls (Rhizopoulou et al., Reference Rhizopoulou, Meletiou-Christou and Diamantoglou1991), drought-tolerant angiosperms (Loewenstein and Pallardy, Reference Loewenstein and Pallardy1998a, Reference Loewenstein and Pallardyb), both Arabica (Tesfaye et al., Reference Tesfaye, Razi and Marziah2008) and Robusta coffees (Tesfaye et al., Reference Tesfaye, Ismail, Kausar, Marziah and Ramlan2013a), grapevines (de Souza et al., Reference de Souza, Maroco, dos Santos, Rodrigues, Lopes, Pereira and Chaves2003, dos Santos et al., Reference dos Santos, Lopes, Rodrigues, de Souza, Maroco, Pereira, Silva and Chaves2003) and common bean (Wakrim et al., Reference Wakrim, Wahbi, Tahi, Aganchich and Serraj2005).

Consistent with the results of the present study, the findings of Ismail et al. (Reference Ismail, Aziz and Hashim1994) on two durian clones have also shown that the rate of decline in LWP, gs and PN during drought stress period greatly varies with species or genotype (whether drought-sensitive or tolerant).

On the other hand, unlike LWP, the rate of decline in gs was faster during the early stages of the stress period until day 3 and then it gradually decreased for clones IC-2, IC-4, IC-8 and R-4. But clones IC-3 and IC-6 showed similar trend and relatively constant rate of decline in both LWP and gs during the stress period. Besides, the rate of decrease in net PN also followed the same trend as observed for gs in IC-2, IC-3 and IC-4, and for gs and LWP in IC-6. In line with this, the decline in net PN of drought-stressed plants has been mostly attributed to the reduced gs (Ismail and Dalia, Reference Ismail and Dalia1995; Pugnaire et al., Reference Pugnaire, Serrano, Pardos and Pessarakli1999), as it has been reported that soil moisture depletion normally decreases the rate of net PN due to its adverse effect on gs of C4 plants, such as maize (Kang et al., Reference Kang, Liang, Hu and Zhang1998; Nagy et al., Reference Nagy, Tuba, Csintalan and Zs1993), durian clones (Ismail et al., Reference Ismail, Aziz and Hashim1994) and drought-sensitive species like some woody angiosperms (Loewenstein and Pallardy, Reference Loewenstein and Pallardy1998a, Reference Loewenstein and Pallardyb).

Stomatal closure in response to soil drying has also been reported for pepper (Awad, Reference Awad2001), grapevine (Stoll et al., Reference Stoll, Loveys and Dry2000) and tomatoes (Hassan et al., Reference Hassan, Mohd Razi, Mohd Mokhtaruddin and Halimi2003; Mingo et al., Reference Mingo, Theobald, Bacon, Davies and Dodd2004). In most cases, decline in turgor pressure and, thus gs and PN of plants subjected to a drying soil, is attributed to decrease in plant water status, indicating stomatal regulation by hydraulic signals (Auge and Moore, Reference Auge and Moore2002; Tardieu and Davies, Reference Tardieu and Davies1993), especially in drought-tolerant or less sensitive species or genotypes (Gaff and Loveys, Reference Gaff and Loveys1992; Loewenstein and Pallardy, Reference Loewenstein and Pallardy1998a, Reference Loewenstein and Pallardyb; Volkmar and Woodbury, Reference Volkmar, Woodbury and Pessarakli1995). In contrast, as discussed earlier, it has been observed that gs was affected by soil drying without a significant reduction in leaf water status in apple (Gowing et al., Reference Gowing, Davies and Jones1990), common bean (Trejo and Davies, Reference Trejo and Davies1991), soybean (Liu et al., Reference Liu, Jensen and Andersen2003), tomatoes (Hassan et al., Reference Hassan, Mohd Razi, Mohd Mokhtaruddin and Halimi2003; Mingo et al., Reference Mingo, Theobald, Bacon, Davies and Dodd2004; Zegbe-Dominguez et al., Reference Zegbe-Dominguez, Behboudian, Lang and Clothier2003) and Arabica coffee seedlings (Tesfaye et al., Reference Tesfaye, Razi and Marziah2008). As observed in this study as well as in some earlier studies, such a stomatal response to water stress in advance of a measurable change in LWP could occur during the early stages of stress development and may result in the reduced rate of PN in drought-sensitive genotypes (Loewenstein and Pallardy, Reference Loewenstein and Pallardy1998a, Reference Loewenstein and Pallardyb; Saliendra et al., Reference Saliendra, Sperry and Comstock1995), probably as a result of chemical signals produced by the roots and transported in the xylem sap to the leaves of drought-stressed plants (Bacon et al., Reference Bacon, Wilkinson and Davies1998; Davies et al., Reference Davies, Tardieu and Trejo1994; Tardieu and Davies, Reference Tardieu and Davies1992; Thompson et al., Reference Thompson, Wilkinson, Bacon and Davies1997). Roots in drying soils produce a drought stress signaling biochemical known as abscissic acid (ABA). This root-sourced plant hormone is transported to the shoot system, particularly to the leaves, and causes stomatal closure (reduction in stomatal opening), and thus reduces rate of loss of water from the plant system through transpiration. In line with this, it has been reported that increase in root-sourced ABA concentration (Davies et al., Reference Davies, Tardieu and Trejo1994; Naqvi, Reference Naqvi and Pessarakli1999; Stoll et al., Reference Stoll, Loveys and Dry2000; Tardieu et al., Reference Tardieu, Katerji, Bethenod, Zhang and Davies1991) and/or changes in pH of the xylem sap (Bacon et al., Reference Bacon, Wilkinson and Davies1998; Gollan et al., Reference Gollan, Schurr and Schulze1992; Kirda et al., Reference Kirda, Cetin, Dasgan, Topcu, Kaman, Ekici, Derici and Ozguven2004; Thompson et al., Reference Thompson, Wilkinson, Bacon and Davies1997) may be responsible for triggering stomatal closure in plants subjected to drought stress.

On the other hand, PN rate of coffee clones IC-2, IC-3, IC-4 and R-4 declined to negative values while plants of clones IC-6 and IC-8 experienced a positive carbon balance under severe water stress reflected by a similar low LWP and gs at the end of the soil drying period. In agreement with these results, Adam and Barakbah (Reference Adam and Barakbah1990) reported that banana plants exhibited a slow decline in PN rate and maintained a positive carbon balance despite complete stomatal closure, but rice plants with partial stomatal opening had a negative value of PN during a severe water stress period, indicating greater sensitivity of rice to drought compared with banana plants. Ismail et al. (Reference Ismail, Aziz and Hashim1994) have also observed a decline in PN rate of a drought-sensitive durian clone to a negative value probably due to a higher stomatal resistance under severe water stress and maintenance of photosynthetic activity at the same degree of stress, although at a slower rate in plants of a drought-tolerant clone. It has been suggested that such differential PN and gs responses of genotypes to drought stress may be associated with factors controlling PN at both stomatal and non-stomatal levels (probably at mesophyll level, the soft parenchyma tissue containing chlorophyll between the epidermal layers of the leaf, which may also be involved in gas exchange in the plant system, especially photosynthetic CO2 fixation by chlorophyll in leaves) (Nagy et al., Reference Nagy, Tuba, Csintalan and Zs1993; Pugnaire et al., Reference Pugnaire, Serrano, Pardos and Pessarakli1999; Volkmar and Woodbury, Reference Volkmar, Woodbury and Pessarakli1995). It has been reported that net PN in coffee was more related to mesophyll conductance than to total gs (Barros et al., Reference Barros, da, Damatta and Maestri1997), although it has been observed that the PN rate in coffee leaves increased with increasing gs up to about 120 mmol m−2 s−1 (Fanjul et al., Reference Fanjul, Arreola-R and Mendez-C.1985). Besides, the rate of decrease in PN rate and the mechanism involved in its depression may vary with the severity of stress. As it has been reported by Lawlor (Reference Lawlor and Smirnoff1995) and Lima et al., (Reference Lima, DaMatta, Pinheiro, Totola and Loureiro2002), the rate of PN decreases due to stomatal closure under moderate water deficit conditions, but biochemical constraints, mainly photoinhibition and photo-oxidative damages to the photosynthetic apparatus because of absorption of excess excitation energy and formation of active oxygen species (Navari-Izzo and Rascio, Reference Navari-Izzo, Rascio and Pessarakli1999; Smirnoff, Reference Smirnoff and Smirnoff1995), may limit the photosynthetic CO2 fixation more directly as the stress progresses.

Besides, the rate of recovery in LWP, gs and PN after rewatering also varied among coffee clones, and it was rapid in IC-2, IC-3, IC-4 and IC-6 compared with IC-8 and R-4. Similar results have been reported by Barros et al. (Reference Barros, da, Damatta and Maestri1997), where application of irrigation increased LWP of water-stressed coffee plants, and thus irrigated plants were sustained under a more favourable water status, while the non-irrigated ones suffered a greater water stress as indicated by the reduced LWP during the peak dry season. Genetic variation in the rate of recovery in LWP and an increase in plant water status during relief from drought have also been reported for different evergreen sclerophylls (Rhizopoulou et al., Reference Rhizopoulou, Meletiou-Christou and Diamantoglou1991). Hence, differences between coffee clones for rate of recovery in gs after rewatering could be associated with variations in the rate of maintenance of LWP, and thus turgidity (Rhizopoulou et al., Reference Rhizopoulou, Meletiou-Christou and Diamantoglou1991; Volkmar and Woodbury, Reference Volkmar, Woodbury and Pessarakli1995). It is also possible that chemical signals, probably accumulated during the stress period, might have been diluted in the transpiration stream by the increased soil and plant water status, thus causing signal concentration to be too low to induce stomatal closure in those coffee clones that exhibited complete recovery in gs six days after rewatering. Increases in the level of growth substances of gibberellins type by increased plant water status (Naqvi, Reference Naqvi and Pessarakli1999) might have also overcome the inhibitory effects of the high levels of ABA in water-stressed plants, and hence might have improved gs during the rewatering phase, although the concentration of these substances has not been measured in the course of the present study. On the other hand, differences between coffee clones for the rate of recovery in net PN could be associated with variations in the rate of increase in gs probably as a consequence of increased LWP, since the rate of PN, gs and LWP followed a similar trend of increase particularly in clones IC-2, IC-3, IC-4 and IC-6 just after rewatering. These results were quite in agreement with the findings of Nagy et al. (Reference Nagy, Tuba, Csintalan and Zs1993), who suggested that stomatal limitation plays the main role in regulating net PN and transpiration during a prolonged drought and after rewatering in maize plants.

The rate of increase in gs after rewatering was, however, much smaller than the rate of increase in LWP and PN in IC-8 and R-4. Increase in PN despite significantly lower gs in these clones might be attributed to increased non-stomatal (mesophyll) conductance, probably because of increase in LWP. Consistent with this, Barros et al. (Reference Barros, da, Damatta and Maestri1997) have reported that net PN in coffee leaves was more related to mesophyll conductance than to gs. It is also possible that differential responses of coffee clones to photoinhibitory and photo-oxidative damages during drought (Karpinski et al., Reference Karpinski, Reynolds, Karpinska, Wingsle, Creissen, Mullineaux, Smallwood, Calvert and Bowles1999; Lawlor, Reference Lawlor and Smirnoff1995; Smirnoff, Reference Smirnoff and Smirnoff1995) and variations in the rate of recovery of the entire photosynthetic apparatus upon rewatering (Lima et al., Reference Lima, DaMatta, Pinheiro, Totola and Loureiro2002; Navari-Izzo and Rascio, Reference Navari-Izzo, Rascio and Pessarakli1999) might have accounted for the observed differences among the clones for the rate of recovery of net PN in the present study. In line with this, it has been reported that attributes of tolerance in a drought-tolerant Robusta coffee clone were linked to less oxidative damages (more protection against photo-oxidation of the photosynthetic apparatus) because of relatively greater increase in the capacity of its antioxidant system compared with that of the drought-sensitive clone (Lima et al., Reference Lima, DaMatta, Pinheiro, Totola and Loureiro2002). Such differences between genotypes for oxidative stress tolerance under water deficit condition and rate of maintenance of photosynthetic apparatus and net PN have also been reported for different resurrection plants and angiosperms (Navari-Izzo and Rascio, Reference Navari-Izzo, Rascio and Pessarakli1999).

Proline accumulation in leaves

Concentration of LP increased as a result of soil drying, and the rate of its increase with increasing time of stress differed between the clones. There was also obvious difference between the clones for proline accumulation even under well-watered condition (Figure 4). Consistent with the results of the present study, Maestri et al. (Reference Maestri, Da Matta, Regazzi and Barros1995) have observed differences among coffee cultivars for the concentration of LP under both control and drought-stressed conditions, but stressed plants accumulated substantially higher level than the non-stressed ones. In his review article, Heuer (Reference Heuer and Pessarakli1999) has also indicated that drought stress imposed by withholding irrigation induced proline accumulation in the leaves of sorghum, wheat, barley, maize, cotton, sunflower, tomato, sugar beets, teff and coffee. Similarly, the accumulation of LP in plants grown under drought stress conditions has also been reported for durian (Ismail et al., Reference Ismail, Aziz and Hashim1994) and tomato (Hassan et al., Reference Hassan, Mohd Razi, Mohd Mokhtaruddin and Halimi2003), as well as for different evergreen sclerophyll species (Rhizopoulou et al., Reference Rhizopoulou, Meletiou-Christou and Diamantoglou1991). In line with this, several workers have correlated drought tolerance with increase in LP concentration during genotype screening, as proline is believed to be one of the most important compatible solutes that play a vital role in osmotic adjustment in crops subjected to water deficit and salinity stresses (Bates et al., Reference Bates, Waldren and Teare1973; Hassan et al., Reference Hassan, Mohd Razi, Mohd Mokhtaruddin and Halimi2003; Pugnaire et al., Reference Pugnaire, Serrano, Pardos and Pessarakli1999). Hence, the rate of accumulation of proline has been regarded as a tolerance mechanism and adaptation of genotypes to drought stress through osmoregulation in a number of crops, including sorghum (Volkmar and Woodbury, Reference Volkmar, Woodbury and Pessarakli1995), tomato (Hassan et al., Reference Hassan, Mohd Razi, Mohd Mokhtaruddin and Halimi2003), durian (Ismail et al., Reference Ismail, Aziz and Hashim1994), wheat (Heuer, Reference Heuer and Pessarakli1999; Volkmar and Woodbury, Reference Volkmar, Woodbury and Pessarakli1995) and banana (Siamak et al., Reference Siamak, Sariah, Zakaria, Sreeramanan and Maziah2012). Ismail et al. (Reference Ismail, Aziz and Hashim1994) and Siamak et al. (Reference Siamak, Sariah, Zakaria, Sreeramanan and Maziah2012) have also reported that the concentration of LP was significantly higher in a drought-tolerant durian and banana clones, respectively, than in the drought-sensitive ones. Similarly, review of relevant literature by Heuer (Reference Heuer and Pessarakli1999) has indicated that free proline production and accumulation was correlated with varietal differences in osmoregulation and thus stress tolerance in alfalfa, safflower, Indian mustard, rice and wheat cultivars. A good correlation between the concentrations of proline and osmotic potential, osmotic adjustment at zero turgor and plant water potential has also been observed with varietal differences for these parameters in both Arabica and Robusta coffees (Maestri et al., Reference Maestri, Da Matta, Regazzi and Barros1995).

In the present study, clones IC-6 and IC-3 exhibited a more rapid and much higher accumulation of LP compared with other coffee clones just following the commencement of soil drying and throughout the water stress period. Increase in proline accumulation in leaves of drought-stressed coffee plants was associated with decrease in LWP, and thus a mechanism of osmotic adjustment in cells or maintenance of turgidity to overcome the stress. Therefore, differences between coffee clones for the level and rate of proline accumulation with increasing time of water stress might be associated with variations for osmotic adjustment and drought tolerance. In line with this, it has been suggested that the accumulation of proline could be used as a single parameter to measure physiological dryness in plants (Bates et al., Reference Bates, Waldren and Teare1973), and thus the rapidity of its accumulation in leaf tissue following the onset of dehydration could be regarded as one of the indicatives of osmoregulation in drought-tolerant genotypes (Ismail et al., Reference Ismail, Aziz and Hashim1994; Siamak et al., Reference Siamak, Sariah, Zakaria, Sreeramanan and Maziah2012).

Coffee clones also showed variations in the rate of decrease in LP concentration as a result of rewatering. The concentration of proline declined sharply and reached a level similar to that of the non-stressed control plants for IC-6 and IC-3, while it was still higher in drought-stressed plants than in the controls, especially for clones IC-8 and R-4 even six days after rewatering (Figure 4). In agreement with these results, Heuer (Reference Heuer and Pessarakli1999) has reviewed that the level of drought stress-induced proline accumulation in the leaves of sorghum plants decreased by rewatering, but remained relatively high when compared with non-stressed controls. On the other hand, the concentration of proline in drought-stressed wheat and cotton plants dropped to the control level within two days after rewatering (Heuer, Reference Heuer and Pessarakli1999). In general, lower rate of proline accumulation in irrigated control plants and drastic reduction of its concentration with rewatering after stress might be associated with its oxidation, as plants can convert free proline into glutamic acid through series of biochemical reactions under non-stress conditions (Heuer, Reference Heuer and Pessarakli1999). It is also possible that the accumulated proline during the stress period might have been used as a source of respiratory energy and nitrogen (N) during the rewatering phase (Heuer, Reference Heuer and Pessarakli1999).

Leaf folding and rate of leaf fall

In general, extent of wilting (mean stress rating) (Figure 5), mean percentage of wilted plants (Figure 6) and rate of leaf fall (Table 2) were considerably higher for IC-2, IC-4, IC-8 and R-4 than for IC-3 and IC-6 during the water stress period. Lower rate of stress development (less wilting symptom and gradual leaf rolling, cupping or folding) as a result of maintenance of turgor has been used as an important criterion during screening of genotypes of mungbean (Vigna radiate) (Rosario et al., Reference Rosario, Ocampo, Sumague, Paje and Kuo1992), soybean (Carter and Rufty, Reference Carter, Rufty and Kuo1992) and rice (Lilley and Fukai, Reference Lilley and Fukai1994; Price et al., Reference Price, Jalaluddin, Dilday and Kuo1992) for drought tolerance. Besides, maintenance of turgor and stay-green under water stress conditions has also been used as one of the traits to select corn varieties for drought tolerance (Kitbamroong and Chantachume, Reference Kitbamroong and Chantachume1992). As observed in the present study, differences among coffee clones for the rate of stress development, expressed by the extent of wilting and damage on leaves, and the corresponding visual stress score value might be attributed to variations in osmotic adjustment as a result of proline accumulation in leaves. In line with this, clones IC-3 and IC-6, which exhibited lower mean stress score (lower rate of stress development), rate of leaf fall and percentage of wilting plants, and thus maintained higher total leaf area and leaf turgidity, had considerably higher LP accumulation (Figure 4) during the drought stress period. Maintenance of turgor by osmotic adjustment is believed to be one of the most important mechanisms of adaptation (Maestri et al., Reference Maestri, Da Matta, Regazzi and Barros1995; Meinzer et al., Reference Meinzer, Saliendra and Crisosto1992; Pugnaire et al., Reference Pugnaire, Serrano, Pardos and Pessarakli1999), allowing plant growth (Heuer, Reference Heuer and Pessarakli1999) and reducing the rate of leaf senescence (stay-green trait or slower-than-normal wilting pattern) by increasing both avoidance and tolerance of dehydration under drought stress conditions (Carter and Rufty, Reference Carter, Rufty and Kuo1992; Joshi, Reference Joshi and Pessarakli1999; Siamak et al., Reference Siamak, Sariah, Zakaria, Sreeramanan and Maziah2012).

Growth and dry matter yield

On the other hand, coffee clones with lower values of stress score and rate of leaf fall exhibited higher rate of recovery upon rewatering, higher vegetative growth (plant height, girth and total leaf area), total dry matter yield and root to shoot ratio and lower level of loss in total leaf area and total dry matter yield than those clones that showed sever wilting and leaf fall. The same trend was also observed under field condition for some vegetative growth parameters (plant height, girth and canopy diameter) and stress score values. Consistent with the results of the present study, it has been reported that leaf scorching scores due to drought stress were inversely correlated with vegetative growth parameters in different Arabica (Tesfaye et al., Reference Tesfaye, Ismail, Kausar, Marziah and Ramlan2013b) and Robusta (Anim-Kwapong et al., Reference Anim-Kwapong, Anim-Kwapong and Adomako2011) coffee genotypes and can be used as a criteria to screen drought-tolerant materials.

CONCLUSIONS

The rate of stress development, decline in LWP, gs and PN and accumulation of proline during water stress period and changes in these parameters after rewatering greatly differed among coffee clones. The clones also varied with respect to vegetative growth, dry matter yield and root to shoot ratio. The rate of stress development in clones IC-6 and IC-3 was generally lower than its values for the other clones. These clones also exhibited quite a better performance in almost all the parameters considered in this study and seemed to be less sensitive to drought stress than did IC-2, IC-4, IC-8 and R-4 under both controlled and field conditions. Drought tolerance attributes in these clones could be linked to a more effective osmotic adjustment due to more rapid accumulation of proline in their leaves. Besides, some morphological traits, mainly increased root to shoot ratio, might have played a role in the response of these clones to soil drying. Therefore, it appears that both physiological and biochemical as well as morphological parameters are equally important and need due consideration during screening crop genotypes for drought tolerance. However, further molecular studies, detailed biochemical, physiological and morphological investigations and observations under diverse field conditions may be required to come up with more conclusive recommendations.

Acknowledgements

The authors wish to acknowledge the Ministry of Higher Education, Malaysia, “Fundamental Research Grant Scheme (FRGS)” for the financial support.


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EFFECT OF SOIL DRYING ON RATE OF STRESS DEVELOPMENT, LEAF GAS EXCHANGE AND PROLINE ACCUMULATION IN ROBUSTA COFFEE (COFFEA CANEPHORA PIERRE EX FROEHNER) CLONES
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EFFECT OF SOIL DRYING ON RATE OF STRESS DEVELOPMENT, LEAF GAS EXCHANGE AND PROLINE ACCUMULATION IN ROBUSTA COFFEE (COFFEA CANEPHORA PIERRE EX FROEHNER) CLONES
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EFFECT OF SOIL DRYING ON RATE OF STRESS DEVELOPMENT, LEAF GAS EXCHANGE AND PROLINE ACCUMULATION IN ROBUSTA COFFEE (COFFEA CANEPHORA PIERRE EX FROEHNER) CLONES
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