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O-34 - Phosphorylation of Leukocyte Glucocorticoid Receptor as a Measure of Stress Vulnerability in Healthy Women and Men

Published online by Cambridge University Press:  15 April 2020

N.P. Maric ,
I. Simic ,
M. Adzic ,
D. Savic ,
J. Djordjevic ,
M. Mihaljevic ,
M. Mitic ,
Z. Pavlovic ,
I. Soldatovic  and
M. Krstic-Demonacos
...Show all authors
N.P. Maric
Affiliation:
Clinic for Psychiatry CCS and Faculty of Medicine Uni Belgrade
I. Simic
Affiliation:
Laboratory of Molecular Biology and Endocrinology VINCA Institute of Nuclear Sciences
M. Adzic
Affiliation:
Laboratory of Molecular Biology and Endocrinology VINCA Institute of Nuclear Sciences
D. Savic
Affiliation:
Laboratory of Theoretical Physics, VINCA Institute of Nuclear Sciences
J. Djordjevic
Affiliation:
Laboratory of Molecular Biology and Endocrinology VINCA Institute of Nuclear Sciences
M. Mihaljevic
Affiliation:
Clinic for Psychiatry CCS
M. Mitic
Affiliation:
Laboratory of Molecular Biology and Endocrinology VINCA Institute of Nuclear Sciences
Z. Pavlovic
Affiliation:
Clinic for Psychiatry CCS
I. Soldatovic
Affiliation:
Faculty of Medicine UNI Belgrade, Belgrade, Serbia
M. Krstic-Demonacos
Affiliation:
Faculty of Life Sciences, University of Manchester, Manchester, UK
M. Jasovic-Gasic
Affiliation:
Clinic for Psychiatry CCS and Faculty of Medicine Uni Belgrade
M. Radojcic
Affiliation:
Laboratory of Molecular Biology and Endocrinology VINCA Institute of Nuclear Sciences

Abstract

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Introduction

The core mechanism of maladaptive stress response includs aberrant phosphorylation of the glucocorticoid receptor (GR) at serine 221 (pGR211) or GR serine 226 (pGR226), which enhance or attenuate GR transcriptional activity, respectively. In the current study we tested a hypothesis that alterations in the nuclear levels and/or ratios of GR, pGR211 and pGR226 in peripheral blood mononuclear cells (PBMC) may reflect individual level of distress and/or psychological vulnerability.

Methods

In 36 healthy adults we evaluated the relation of total PMBC nuclear level of GR (tGR), pGR221, pGR226 and activated JNK1 (pJNK1) and its relation to environmental stress exposure, self reported distress (Depression Anxiety Stress Scale) and personality traits (Eysenck Personality Questionnaire). Molecular and psychometric data were cross-correlated by SPSS MannWhitney statistics.

Results

In women, self reported distress (DASS score) correlated more with neuroticism, while in men it correlated with environmental stressor. Also, in women, PBMC nuclear tGR level correlated with pGR211, while in men it correlated with pGR226. Cross-analysis of psychometric and molecular data indicated that in women both neuroticism and self reported distress correlated positively with nuclear pGR226, while in men the only correlation was between environmental stressor and pJNK1.

Conclusion

This findings provide preliminary support that the nuclear level of pGR226 and pGR211/pGR226 ratio in PBMC may serve as a measure of affective vulnerability in healthy women, while nuclear level of pJNK1 may reflect environmentaly imposed distress in healthy men. The relevance of these molecular parameters as biomarkers requires broader verification in healthy and in clinical settings.

Type
Abstract
Information
European Psychiatry , Volume 27 , Issue S1: Abstracts of the 20th European Congress of Psychiatry , 2012 , pp. 1
Copyright
Copyright © European Psychiatric Association 2012
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