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European Journal of Anaesthesiology European Journal of Anaesthesiology

Article contents

The influence of propofol on the expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in reoxygenated human umbilical vein endothelial cells

Published online by Cambridge University Press:  11 July 2006

T. B. Corcoran ,
A. Engel  and
G. D. Shorten
T. B. Corcoran
Affiliation:
Cork University Hospital, Department of Anaesthesia, Cork City, Republic of Ireland University College Cork, Department of Anaesthesia, Cork City, Republic of Ireland
A. Engel
Affiliation:
Cork University Hospital, Department of Anaesthesia, Cork City, Republic of Ireland
G. D. Shorten
Affiliation:
Cork University Hospital, Department of Anaesthesia, Cork City, Republic of Ireland University College Cork, Department of Anaesthesia, Cork City, Republic of Ireland
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Abstract

Summary

Background: Leucocytes are a pivotal component of the inflammatory cascade that results in tissue injury in a large group of disorders. Free radical production and endothelial activation promote leucocyte–endothelium interactions via endothelial expression of vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) which augment these processes, particularly in the setting of reperfusion injury. Propofol has antioxidant properties which may attenuate the increased expression of these molecules that is observed. Methods: Cultured human umbilical vein endothelial cells were exposed to 20 h of hypoxia, then returned to normoxic conditions. Cells were treated with saline, Diprivan® 5 μg mL−1 or propofol 5 μg mL−1, for 4 h after reoxygenation and were examined for ICAM-1 and VCAM-1 expression. Results: Hypoxia did not increase the expression of ICAM-1/VCAM-1. ICAM-1 expression peaked 12 h after reoxygenation (21.75(0.6) vs. 9.6(1.3), P = 0.02). Propofol, but not Diprivan®, prevented this increase (8.2(2.9) vs. 21.75(0.6), P = 0.009). VCAM-1 expression peaked 24 h after reoxygenation (9.8(0.9) vs. 6.6(0.6), P = 0.03). Propofol and Diprivan® prevented this increase, with no difference between the two treatments observed (4.3(0.3) and 6.4(0.5) vs. 9.8(0.9), P = 0.001, 0.02, respectively). Conclusion: These effects are likely to be attributable to the antioxidant properties of propofol, and suggest that propofol may have a protective role in disorders where free radical mediated injury promotes leucocyte–endothelium adhesive interactions.

Keywords

LEUCOCYTES ENDOTHELIUM VASCULAR PROPOFOL FREE RADICALS REPERFUSION INJURY ADHESION MOLECULES
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 23 , Issue 11 , November 2006 , pp. 942 - 947
Copyright
2006 European Society of Anaesthesiology

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The influence of propofol on the expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in reoxygenated human umbilical vein endothelial cells
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