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The use of SDS-polyacrylamide gel electrophoresis in epidemiological studies of Corynebacterium diphtheriae

Published online by Cambridge University Press:  19 October 2009

Gillian Hallas
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, Colindale Avenue, London NW9 5HT, UK
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Summary

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Polyacrylamide gel electrophoresis of cell proteins was investigated as a possible typing method for Corynebacterium diphtheriae. A method was developed using stock strains which were representatives of the five gravis serotypes described by Robinson & Peeney (1936). This technique was then applied to recent isolates sent to our laboratory for identification.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1988

References

REFERENCES

Davies, J. R. (1974). Elek's test for the toxigenicity of Corynebacteria. Public Health Laboratory Service Monograph Series no. 5, Laboratory Methods 1 (eds. Willis, A. T. & Collins, C. H.), pp. 17. London: HMSO.Google Scholar
Gibson, L. F. & Colman, G. (1973). Diphthericin types, bacteriophage types and serotypes of Corynebacterium diphtheriae strains isolated in Australia. Journal of Hygiene 71, 679689.Google Scholar
Jackman, P. J. H. (1982). Classification of Corynebacterium species from axillary skin by numerical analysis of electrophoretic protein patterns. Journal of Medical Microbiology 15, 485492.Google Scholar
Jackman, P. J. H. & Pelczynska, S. (1986). Characterization of Corynebacterium Group JK by whole-cell protein patterns. Journal of General Microbiology 132, 19111915.Google Scholar
Keen, A. P. & Mitchell, R. G. (1986). Commercial strip test for reduction of nitrate by bacteria. Journal of Clinical Pathology 39, 118.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680685.Google Scholar
Larsen, S. A., Bickham, S. T., Buchanan, T. M. & Jones, W. L. (1971). Polyacrylamide gel electrophoresis of Corynebacterium diphtheriae: a possible epidemiological aid. Applied Microbiology 22, 885890.CrossRefGoogle ScholarPubMed
Palva, E. T. & Mäkelä, P. H. (1980). Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. European Journal of Biochemistry 107, 137143.CrossRefGoogle ScholarPubMed
Robinson, D. T. & Peeney, A. L. P. (1936). The serological types amongst gravis strains of C. diphtheriae and their distribution. Journal of Pathology and Bacteriology 43, 403418.CrossRefGoogle Scholar
Russell, R. R. B. (1976). Classification of Streptococcus mutans strains by SDS gel electrophoresis. Microbios Letters 2, 5559.Google Scholar
Saragea, A., Maximescu, P. & Meitert, E. (1979). Corynebacterium diphtheriae: Microbiological methods used in clinical and epidemiological investigations. In Methods in Microbiology, vol. 13 (ed. Bergan, T. & Norris, J. R.), pp. 61176. London: Academic Press.Google Scholar