Hostname: page-component-8448b6f56d-cfpbc Total loading time: 0 Render date: 2024-04-23T15:22:44.376Z Has data issue: false hasContentIssue false
  • English
  • Français

Typing of Pseudomonas aeruginosa: comparison of the phage procedure with the pyocine technique

Published online by Cambridge University Press:  15 May 2009

Ruth Bernstein-Ziv ,
Rose Mushin  and
Kurt Rabinowitz
Ruth Bernstein-Ziv
Affiliation:
Asaf Harofe Government Hospital, Zerifin, Israel
Rose Mushin
Affiliation:
Kimron Veterinary Institute, Beit-Dagan, Israel
Kurt Rabinowitz
Affiliation:
Ministry of Health, Jerusalem, Israel
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two hundred and sixty strains of Pseudomonas aeruginosa were isolated from patients of the Asaf Harofe Government Hospital. The strains were typed by phage technique and 64 of them were also typed according to pyocine sensitivity. The two methods proved complementary, and reduced the number of untypable strains. Phage typing was performed with both routine test dilution (RTD) and more concentrated phage suspensions. The most reliable results were obtained at 100 RTD.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 2 , June 1973 , pp. 403 - 410
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Ayliffe, G. A. J., Lowbury, E. J. L., Hamilton, J. G., Small, J. M., Asheshov, E. A. & Parker, M. T. (1965). Hospital infection with Pseudomonas aeruginosa in neurosurgery. Lancet ii, 365.CrossRefGoogle Scholar
Ayliffe, G. A. J., Barry, D. R., Lowbury, E. J. L., Roper-Hall, M. J. & Walker, W. M. (1966). Post-operative infection with Pseudomonas aeruginosa in an eye hospital. Lancet i, 1113.CrossRefGoogle Scholar
Bergan, T. (1972). A new bacteriophage typing set for Pseudomonas aeruginosa. 2. Characterization and comparison of new and old previous typing set. Acta pathologica et microbiologica scandinavica 80, 189.Google Scholar
Borst, J. & de Jong, J. H. L. (1970). Serological typing of Pseudomonas aeruginosa. Antonie van Leeuwenhoek 36, 190.Google Scholar
Deighton, M. A., Tagg, J. R. & Mushin, R. (1971). Epidemiology of Pseudomonas aeruginosa infection in hospitals. 2. ‘Fingerprints’ of Pseudomonas aeruginosa strains in study of cross-infection in a children's hospital. Medical Journal of Australia i, 892.CrossRefGoogle Scholar
Farmer, J. J. & Herman, L. G. (1969). Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity to pyocine and bacteriophage. Applied Microbiology 18, 760.CrossRefGoogle ScholarPubMed
Fierer, J., Taylor, P. M. & Gezon, H. M. (1967). Pseudomonas aeruginosa epidemic traced to delivery room resuscitators. New England Journal of Medicine 276, 991.CrossRefGoogle ScholarPubMed
Gillies, R. R. & Govan, J. R. W. (1966). Typing of Pseudomonas pyocyanea by pyocine production. Journal of Pathology and Bacteriology 91, 339.CrossRefGoogle ScholarPubMed
Govan, J. R. W. & Gillies, R. R. (1969). Further studies in the pyocine typing of Pseudomonas pyocyanea. Journal of Medical Microbiology 2, 17.CrossRefGoogle ScholarPubMed
Graber, C. D., Latta, R., Vogel, E. H. & Brame, R. (1962). Bacteriophage grouping of Pseudomonas aeruginosa with special emphasis on lysotypes occurring in infected burns. American Journal of Clinical Pathology 37, 54.CrossRefGoogle ScholarPubMed
Hardy, P. C., Ederrer, G. M. & Matsen, J. M. (1970). Contamination of commercially packaged urinary catheter kits with pseudomonad EO-1. New England Journal of Medicine 282, 1.CrossRefGoogle ScholarPubMed
Habs, I. (1957). Untersuchungen über die O-antigene von Pseudomonas aeruginosa. Zeitschrift für Hygiene und Infektionskrankheiten 144, 218.CrossRefGoogle ScholarPubMed
Hobbs, G. D. C., Gowland, G. & Byers, H. D. (1964). A serological approach to the genus Pseudomonas. Applied Microbiology 27, 83.Google Scholar
Lindberg, R. B., Curreri, P. W. & Pruitt, J. (1970). Microbiology of burns treated with carbenicillin. Experimental and clinical observations. Journal of Infectious Diseases 122, s34.CrossRefGoogle Scholar
Matsumoto, H., Tazaki, T. & Kato, T. (1968). Serological and pyocine types of Pseudomonas aeruginosa from various sources. Japanese Journal of Microbiology 12, 111.CrossRefGoogle ScholarPubMed
Moffet, H. L. & Williams, T. (1967). Bacteria recovered from distilled water and inhalation therapy equipment. American Journal of Diseases of Children 114, 7.Google ScholarPubMed
Stone, H. H., Kalb, L. P. & Joseph, L. B. (1971). The evolution and spread of gentamycin-resistant Pseudomonas. Journal of Trauma 11, 586.CrossRefGoogle Scholar
Shooter, R. A., Gaga, H., Cook, E. M. & Kumar, P. (1969). Food and medicaments as possible sources of hospital strains of Pseudomonas aeruginosa. Lancet i, 1227.CrossRefGoogle Scholar
Sjöberg, L. & Lindberg, A. A. (1968). Phage typing of Pseudomonas aeruginosa. Acta pathologica et microbiologica scandinavica 74, 61.CrossRefGoogle ScholarPubMed
Sutter, V. L., Hurst, V. & Fennia, J. (1963). Semisynthetic medium for bacteriophage typing of Pseudomonas. Journal of Bacteriology 86, 1354.CrossRefGoogle ScholarPubMed
Tagg, J. R. & Mushin, R. (1971). Epidemiology of Pseudomonas aeruginosa infection in hospitals. 1. Pyocine typing of Pseudomonas aeruginosa. Medical Journal of Australia i, 847.CrossRefGoogle Scholar
Wahba, A. H. (1963). The production and inactivation of pyocines. Journal of Hygiene 61, 431.Google ScholarPubMed
Weber, G., Werner, H. P. & Matchnigg, H. (1971). Pseudomonas aeruginosa im Trinkwasser als Todessursache bei Neugebornen. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. I (Orig.) 216, 210.Google Scholar
Williams, R., Williams, E. D. & Hyams, D. E. (1960). Cross-infection with Pseudomonas pyocyanea. Lancet i, 376.CrossRefGoogle Scholar