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The typing of enteroviruses in tissue culture by neutralization with composite antiserum pools

  • M. H. Hambling (a1), Patricia M. Davis (a1) and A. D. Macrae (a1)

Extract

A scheme is described for the identification of enteroviruses in tissue culture by neutralization with composite antiserum pools. The method for making these pools is given. Antisera to twenty-seven enteroviruses were included in the pools which were used to examine 115 viruses consisting of ninety-eight recently isolated viruses and seventeen prototype strains. The results indicate that this scheme provides a useful screening method for identifying enteroviruses. It has proved to be practicable, time saving and very economical in tissue culture.

We are grateful to Dr C. M. P. Bradstreet who made available some of the rabbit antisera.

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References

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Karber, G. (1931). Beitrag zur kollektiven Bhandlung pharmakologischer Reihenversuche. Arch. exp. Path. Pharmaic. 162, 480.
Lim, K. A. & Benyesh-Melnick, M. (1960). Typing of viruses by combination of antiserum pools. Application to typing of enteroviruses (Coxsackie and ECHO). J. Immunol. 84, 309.
Melnick, J. L. (1958). Advances in the study of enteroviruses. Proyr. med. Virol. 1, 59.
Report (1962). Classification of human enteroviruses. Virology, 16, 501.
Schmidt, N. J., Guenther, R. W. & Lennette, E. H. (1961). Typing of ECHO virus isolates by immune serum pools. The ‘intersecting serum scheme’;. J. Immunol. 87, 623.

The typing of enteroviruses in tissue culture by neutralization with composite antiserum pools

  • M. H. Hambling (a1), Patricia M. Davis (a1) and A. D. Macrae (a1)

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