Skip to main content Accessibility help
×
Home

Systematic detection and association of Entamoeba species in stool samples from selected sites in India

  • J. NATH (a1) (a2), N. BANYAL (a3), D. S. GAUTAM (a3), S. K. GHOSH (a2), B. SINGHA (a1) and J. PAUL (a3)...

Summary

This study developed a fast and high throughput dot-blot technique to evaluate the presence of Entamoeba in stool samples (n = 643) followed by a PCR-based method to validate and differentiate the two species E. histolytica and E. dispar. The prevalence rate of the parasite has been detected in a cross-sectional study carried out in the population of the Eastern and Northern parts of India. Of the various demographic features, prevalence was highest in the monsoon season (P = 0·017), in the <15 years age group (P = 0·015). In HIV-positive individuals, the prevalence rate was significantly high (P = 0·008) in patients with a CD4 cell count <200 as well as in patients without antiretroviral therapy (ART) (P = 0·011). Our analysis further confirmed that risk factors such as toilet facilities, living conditions, hygienic practices, drinking water source, occupation and level of education are important predictors as they were found to contribute significantly in the prevalence of the parasite.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Systematic detection and association of Entamoeba species in stool samples from selected sites in India
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Systematic detection and association of Entamoeba species in stool samples from selected sites in India
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Systematic detection and association of Entamoeba species in stool samples from selected sites in India
      Available formats
      ×

Copyright

Corresponding author

* Author for correspondence: Dr J. Paul, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. (Email: jpaul33@hotmail.com)

References

Hide All
1. John, CC, Salata, RA. Amebiasis. In: Kliegman, RM, Stanton, B, St. Geme, J, Schor, N, Behrman, RE, eds. Nelson Textbook of Pediatrics. Philadelphia: Saunders, 2010, pp. 11231125.
2. Pham, Duc P, et al. Risk factors for Entamoeba histolytica infection in an agricultural community in Hanam province, Vietnam. Parasites & Vectors 2011; 4: 102.
3. Haque, R, et al. Epidemiologic and clinical characteristics of acute diarrhea with emphasis on Entamoeba histolytica infections in preschool children in an urban slum of Dhaka, Bangladesh. American Journal of Tropical Medicine and Hygiene 2003; 69: 398405.
4. Al-Harthi, S, Jamjoom, M. Diagnosis and differentiation of Entamoeba infection in Makhah Al Mukarramah using microscopy and stool antigen detection kits. World Journal of Medical Sciences 2007; 2: 1520.
5. Norhayati, M, et al. Intestinal parasitic infections in man: a review. Medical Journal of Malaysia 2003; 58: 296305.
6. Ximenez, C, et al. Reassessment of the epidemiology of amebiasis: State of the art Infection. Genetics and Evolution 2003; 9: 10231032.
7. Haque, R, et al. Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits. Journal of Clinical Microbiology 1995; 33: 25582561.
8. Khairnar, K, Parija, SC, Palaniappan, R. Diagnosis of intestinal amoebiasis by using nested polymerase chain reaction-restriction fragment length polymorphism assay. Journal of Gastroenterology 2007; 42: 631640.
9. Petri, WA Jr., et al. Estimating the impact of amoebiasis on health. Parasitology Today 2000; 16: 320321.
10. Fotedar, R, et al. PCR detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in stool samples from Sydney, Australia. Journal of Clinical Microbiology 2007; 45: 10351037.
11. Mirelman, D, Nuchamowitz, Y, Stolarsky, T. Comparison of use of enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes for simultaneous detection of Entamoeba histolytica and E. dispar . Journal of Clinical Microbiology 1997; 35: 24052407.
12. Durack, DT. Opportunistic infections and kaposis sarcoma in homosexual men. New England Journal of Medicine 1981; 305: 1465–467.
13. Savioli, L, et al. Schitosomiasis and soil transmitted helminthics infections: forgoing control efforts. Transactions of the Royal Society of Tropical Medicine and Hygiene 2002; 96: 577579.
14. Nkenfou, CN, Nana, CT, Payne, VK. Intestinal Parasitic Infections in HIV Infected and Non-Infected Patients in a Low HIV Prevalence Region, West-Cameroon. PLoS ONE 2013; 8: e57914.
15. Tanyuksel, M, Petri, WA Jr. Laboratory diagnosis of amebiasis. Clinical Microbiology Reviews 2003; 16: 713729.
16. Daniel, WW. Biostatistics: A Foundation for Analysis in the Health Sciences, 7th edn. New York: John Wiley & Sons, 1999.
17. Knight, WB, et al. A modification of the formol-ether concentration technique for increased sensitivity in detecting Schistosoma mansoni eggs. American Journal of Tropical Medicine and Hygiene 1976; 2: 818823.
18. Srivastava, S, Bhattacharya, S, Paul, J. Species and strain-specific probes derived from repetitive DNA for distinguishing Entamoeba histolytica and Entamoeaba dispar . Experimental Parasitology 2005; 110: 303308.
19. Giles, M, et al. A multiplex allele specific polymerase chain reaction (MAS-PCR) on the dihydrofolate reductase gene for the detection of Cryptosporidium parvum genotypes 1 and 2. Parasitology 2002; 125: 3544.
20. Diamond, LS, Clark, CG. A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. Journal of Eukaryotic Microbiology 1993; 40: 340344.
21. Haque, R, Petri, WA Jr. Diagnosis of amebiasis in Bangladesh. Archives of Medical Research 2006; 37: 273–176.
22. Paul, J, Bhattacharya, A, Bhattacharya, S. Close sequence identity between ribosomal DNA episomes of the non-pathogenic Entamoeba dispar and pathogenic Entamoeba histolytica . Journal of Biosciences 2002; 27: 619627.
23. Anuar, TS, et al. Molecular epidemiology of amoebiasis in Malaysia: highlighting the different risk factors of Entamoeba histolytica and Entamoeba dispar infections among Orang Asli communities. International Journal for Parasitology 2012; 42: 11651175.
24. Ngui, R, et al. Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia. Parasites & Vectors 2012; 5: 187.
25. Adamu, H, Wegayehu, T, Petros, B. High prevalence of diarrhoegenic intestinal parasite infections among non-ART HIV patients in Fitche Hospital, Ethiopia. PLoS ONE 2013; 8: e72634.
26. Morán, P, et al. Infection by human immunodeficiency virus-1 is not a risk factor for amebiasis. American Journal of Tropical Medicine and Hygiene 2005; 73: 296300.
27. Kinuthia, GK, et al. Selected practices among rural residents versus the prevalence of Amoebiasis and Giardiasis in Njoro District, Kenya. African Journal of Health Sciences 2012; 20: 1120.

Keywords

Systematic detection and association of Entamoeba species in stool samples from selected sites in India

  • J. NATH (a1) (a2), N. BANYAL (a3), D. S. GAUTAM (a3), S. K. GHOSH (a2), B. SINGHA (a1) and J. PAUL (a3)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed