Skip to main content Accessibility help
×
Home

The specific IgM response to Mycoplasma pneumoniae infection: interpretation and application to early diagnosis

  • J. H. Moule (a1), E. O. Caul (a1) and T. G. Wreghitt (a2)

Summary

Specific IgM antibody production in patients with serologically proven Mycoplasma pneumoniae infection by the complement fixation (CF) test was studied using a μ-capture ELISA. Sera from 79% of patients were found to be IgM positive. Patients could be divided into two groups relating to the amount of specific antibody produced. High levels of specific IgM (≥10 units) were more commonly found in younger patients. Seventy-six per cent of patients under the age of 20 produced relatively high levels of IgM compared to 35% of patients over the age of 20. In contrast, the number of patients who produced low or undetectable levels of IgM ( < 10 units) was found to increase with age. This trend was found to be significant which suggests that low or undetectable levels of IgM may be due to reinfection with M. pneumoniae. Specific IgM was found to appear in the serum at approximately 7 days after the onset of symptoms, peaking at between 10 and 30 days, and then falling to undetectable levels at an estimated 12–26 weeks post onset of symptoms. Twenty-eight per cent of acute-phase sera (CF titres < 256) from patients whose sera subsequently showed a fourfold or greater rise in M. pneumoniae CF antibody titre were IgM positive. Thus using μ-capture ELISA a diagnosis of M. pneumoniae infection may often be made more rapidly than by the complement fixation test.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      The specific IgM response to Mycoplasma pneumoniae infection: interpretation and application to early diagnosis
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      The specific IgM response to Mycoplasma pneumoniae infection: interpretation and application to early diagnosis
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      The specific IgM response to Mycoplasma pneumoniae infection: interpretation and application to early diagnosis
      Available formats
      ×

Copyright

References

Hide All
Biberfeld, G. (1971). Antibody responses in Mycoplasma pneumoniae infection in relation to serum immunoglobulins, especially Igm. Acta Pathologica et Microbiologica Scandinavica, section B 79, 620634.
Bradstreet, C. M. P. & Taylor, C. E. D. (1962). Technique of complement-fixation test applicable to the diagnosis of virus diseases. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21, 96104.
Chamberlain, P. & Saeed, A. A. (1983). A study of the specific Igm antibody response in Mycoplasma pneumoniae infection in man. Journal of Hygiene 90, 207211.
Chanock, R. M., Hayflick, L. & Barile, M. D. (1962). Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proceedings of the National Academy of Sciences of the United States of America 48, 4149.
Dussaix, E., Slim, A. & Tournier, P. (1983). Comparison of enzyme-linked immunosorbent assay (ELISA) and complement fixation test for detection of Mycoplasma pneumoniae antibodies. Journal of Clinical Pathology 36, 228232.
Forsyth, B. R. & Chanock, R. M. (1966). Mycoplasma pneumonia. Annual Review of Medicine 17, 371382.
Foy, H. M., Kenny, G. E., McMahan, R., Mansy, A. M. & Grayston, J. T. (1970). Mycoplasma pneumoniae pneumonia in an urban area. Journal of the American Medical Association 214, 16661672.
Foy, H. M., Kenny, G. E., Sefi, R., Ochs, H. D. & Allan, I. D. (1977). Second attacks of pneumonia due to Mycoplasma pneumoniae. Journal of Infectious Diseases 135, 673677.
Foy, H. M., Kenny, G. E., Cooney, M. K., Allan, I. D. & Van Belle, G. (1983). Naturally acquired immunity to pneumonia due to Mycoplasma pneumoniae. Journal of Infectious Diseases 147, 967973.
Hu, P. C., Powell, D. A., Albright, F., Gardner, D. E., Collier, A. M. & Clyde, W. A. (1983). A solid-phase radioimmunoassay for detection of antibodies against Mycoplasma pneumoniae. Journal of Clinical and Laboratory Immunology 11, 209213.
Leinikki, P. O., Panzar, P. & Tykka, H. (1978). Immunoglobulin M antibody response against Mycoplasma pneumoniae lipid antigen in patients with acute pancreatitis. Journal of Clinical Microbiology 8, 113118.
Nakamura, S., Ebisawa, L., Kitamoto, O. & Sato, T. (1970). Persistence of serum antibody following Mycoplasma pneumoniae infection. American Review of Respiratory Diseases 101, 620622.
Peto, R., Pike, M. C., Armitage, P., Breslow, N. E., Cox, D. R., Howard, S. V., Mantel, N., McPherson, K., Peto, J. & Smith, P. G. (1977). Design and analysis of randomized clinical trials which require prolonged observations of each patient. 2. Analysis and examples. British Journal of Cancer 35, 139.
Price, P. C. (1980). Direct radioimmunoassay for the detection of Igm antibodies against Mycoplasma pneumoniae. Journal of Immunological Methods 32, 261273.
Raisanen, S. M., Suni, J. & Leinikki, P. (1980). Serological diagnosis of Mycoplasma pneumoniae infection by enzyme immunoassay. Journal of Clinical Pathology 33, 836840.
Sillis, M. & Andrews, B. (1978). A simple test for Mycoplasma pneumoniae Igm. Zentralblatt für Bakteriologie, Mirobiologie, Infektionskrankheiten und Hygiene, Abteilung 1: Originate, Reihe A: Medizinische Mikrobiologie und Parasitologie 241, 239240.
Skaug, K., Eng, J., Orstavik, I. & Haug, K. W. (1976). The diagnostic value of determination of Igm antibodies against Mycoplasma pneumoniae infections by the indirect immunofluorescent antibody test. Acta Pathologica et Microbiologica Scandinavica, section B 84, 170176.
Watson, G. I. (1977). The treatment of Mycoplasma pneumoniae infections. Scottish Medical Journal 22, 331335.
Wreghitt, T. G. & Sillis, M. (1985). A μ-capture ELISA for detecting Mycoplasma pneumoniae Igm: comparison with indirect immunofluorescence and indirect ELISA. Journal of Hygiene 94, 217227.

The specific IgM response to Mycoplasma pneumoniae infection: interpretation and application to early diagnosis

  • J. H. Moule (a1), E. O. Caul (a1) and T. G. Wreghitt (a2)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed