Hostname: page-component-8448b6f56d-qsmjn Total loading time: 0 Render date: 2024-04-23T08:22:16.779Z Has data issue: false hasContentIssue false
  • English
  • Français

Speciation, serotyping, antimicrobial sensitivity and plasmid content of Proteeae from the environment of calf-rearing units in South West England

Published online by Cambridge University Press:  19 October 2009

P. M. Hawkey ,
J. L. Penner ,
A. H. Linton ,
C. A. Hawkey ,
L. J. Crisp  and
M. Hinton
P. M. Hawkey
Affiliation:
Department of Microbiology, University of Bristol, University Walk, Bristol BS8 TD1, England
J. L. Penner
Affiliation:
Department of Microbiology, University of Toronto, Banting Institute, 100 College Street, Toronto, Ontario MG5 1L5, Canada
A. H. Linton
Affiliation:
Department of Microbiology, University of Bristol, University Walk, Bristol BS8 TD1, England
C. A. Hawkey
Affiliation:
Department of Microbiology, University of Bristol, University Walk, Bristol BS8 TD1, England
L. J. Crisp
Affiliation:
Department of Microbiology, University of Toronto, Banting Institute, 100 College Street, Toronto, Ontario MG5 1L5, Canada
M. Hinton
Affiliation:
Department of Veterinary Medicine, University of Bristol, Langford House, Langford, Avon BS18 7DU, England
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A survey was undertaken of the occurrence, serotype, antimicrobial sensitivity and plasmid content of members of the tribe Proteeae in the environment of two calf-rearing units in the county of Avon in South West England. Examples of the following species were found: Proteus mirabilis, Prot. vulgaris, Prot. vulgaris Biogroup 2, Morganella morganii, Providencia stuartii, Prov. alcalifaciens and Prov. rettgeri. A wide range of sero types was found, many having been previously reported from nosocomial isolates. A total of 15% of isolates carried plasmids; six pairs of isolates were identified which had identical sero typesbut different patterns of plasmid carriage. The antimicrobial sensitivity of the isolates was generally similar to isolates of Proteeae from humans. Although no truly aminoglycosideresistantisolates were found, some isolates of Prov. stuartii and Prov. rettgeri had MIC's higher than the other isolates to gentamicin and netilmicin, suggesting the presence of lowlevels of the enzyme AAC 2′. The study demonstrates that there is a considerable diversityof species and types of Proteeae associated with calves and their environment. It seems likely that a potential cause of colonization of the human gut by Proteeae is the consumption of meat.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 3 , December 1986 , pp. 405 - 417
Copyright
Copyright © Cambridge University Press 1986

References

REFERENCES

Anderson, E. S., Humphreys, G. O. & Willshaw, G. A. (1975). The molecular relatedness of R Factors in Enterobacteria of human and animal origin. Journal of General Microbiology 91, 376–382.CrossRefGoogle ScholarPubMed
Chow, A. W., Taylor, P. R., Yoshikawa, T. T. & Guze, L. B. (1979). A nosocomial outbreak of infections due to multiply resistant Proteus mirabilis: role of intestinal colonization as major reservoir. Journal of Infectious Diseases 139, 621–627.Google Scholar
Epidemiological. Research Laboratory of the Public Health Laboratory Service, United Kingdom and Republic of Ireland (1977). Unusual infection in intensive care unit. British Medical Journal i, 111.Google Scholar
Ewing, W. H., Tanner, K. E. & Dennard, D. A. (1954). The Providence group: an intermediate group of enteric bacteria. Journal of Infectious Diseases 94, 134–140.CrossRefGoogle ScholarPubMed
Hawkey, P.M. (1984). Providencia stuartii: a review of a multiply antibiotic-resistant bacterium. Journal of Antimicrobial Chemotherapy 13, 209–226.Google Scholar
Hawkey, P. M., Bennet, P. M. & Hawkey, C. A. (1984). Cryptic plasmids in hospital isolates of Providencia stuartii. Journal of Medical Microbiology 18, 277–284.CrossRefGoogle Scholar
Hawkey, P. M., Bennet, P. M. & Hawkey, C. A. (1985). Evolution of an R plasmid from a cryptic plasmid by transposition of two copies of Tnl in Providencia stuartii. Journal of General Microbiology 131, 927–933.Google Scholar
Hawkey, P.M., Pedler, S. J. & Turner, A. (1983). The comparative in vitro activity of semisynthetic penicillins against Proteeae. Antimicrobial Agents and Chemotherapy 23, 619–621.Google Scholar
Hawkey, P. M., Penner, J. L., Potten, M. R., Stephens, M., Barton, L. J. & Speller, D. C. E. (1982). Prospective survey of faecal, urinary tract, and environmental colonization by Providencia stuartii in two geriatric wards. Journal of Clinical Microbiology 16, 422–426.CrossRefGoogle ScholarPubMed
Hickman, F. W., Stieger Walt, A. G., Farmer, J. J. & Brenner, D. J. (1982). Identification of Proteus penneri sp.nov., formerly known as Proteus vulgaris indole negative or as Proteus vulgaris Biogroup I. Journal of Clinical Microlnology 15, 1097–1102.CrossRefGoogle ScholarPubMed
Hirsh, D. C., Ikeda, J. S., Martin, L. D., Kelly, B. J. & Ghazikhanian, G. Y. (1983). R plasmid-mediated gentamicin resistance in salmonellae isolated from turkeys and their environment. Avian Diseases 27, 766–772.CrossRefGoogle ScholarPubMed
Hollick, G. E., Nolte, F. S., Calnan, B. J., Penner, J. L., Barton, L. J. & Spellacy, A. (1984). Characterization of endemic Providencia stuartii isolates from patients with urinary devices. European Journal of Clinical Microbiology 3, 521–525.CrossRefGoogle ScholarPubMed
Lanyi, B. (1957). Serological typing of Proteus strains. Sensitivities of serotypes to antibiotics. Ada Microbiologica Academiae Scientarum Hungariae 4, 447–457.Google ScholarPubMed
Levine, M. (1942). An ecological study of Proteus. Journal of Bacteriology 43, 33–34.Google Scholar
Linton, A. H. (1977). Antibiotic resistance: The present situation reviewed. Veterinary Record 100, 354–360.CrossRefGoogle ScholarPubMed
Linton, A. H. & Hinton, M. H. (1984). The ecology of antibiotic-resistant bacteria in animals and their environment. In Antimicrobials and Agriculture (ed. Woodbine, M.), pp. 533–549. London: Butterworth.CrossRefGoogle Scholar
Linton, A. H., Howe, K., Bennett, P. M., Richmond, M. H. & Whiteside, E. J. (1977). The colonization of the human gut by antibiotic-resistant Escherichia coli from chickens. Journal of Applied Bacteriology 43, 465–469.Google Scholar
de Louvois, J. (1969). Serotyping and the Dienes reaction on Proteus mirabilis from hospital infections. Journal of Clinical Pathology 22, 263–268.Google Scholar
Meers, P. D., Ayliffe, G. A. J., Emmerson, A. M., Leigh, D. A., Mayor-White, R. T., Mackintosh, C. A. & Stronge, J. L. (1981). Report on the national survey of infection in hospitals, 1980. Journal of Hospital Infection 2 (Suppl.), 1–49.Google Scholar
Namioka, S. & Sakazaki, R. (1958). Etude sur les Rettlgerella. Annales del' Institute Pasteur 94, 485–499.Google Scholar
Penner, J. L. (1981). The Tribe Proteeae. In The Prokaryotes: A Handbook on Habitats, Isolation and Identification of Bacteria (ed. Starr, M. P., Stolp, H., Truper, G., Balows, A., Schlegel, H. G.), pp. 1204–1224. Berlin, Heidelberg: Springer-Verlag.Google Scholar
Penner, J. L. & Hennessy, J. N. (1979 a). Application of O-serotyping in a study of Providencia rettgeri (Proteus rettgeri) isolated from human and non-human sources. Journal of Clinical Microbiology 10, 834–840.CrossRefGoogle Scholar
Penner, J. L. & Hennessy, J. N. (1979 b). O antigen grouping of Morganella morganii (Proteus morganii) by slide agglutination. Journal of Clinical Microbiology 10, 8–13.CrossRefGoogle ScholarPubMed
Penner, J. L., Fleming, P. C, Whiteley, G. R. & Hennessy, J. N. (1979 a). O-serotyping Providencia alcalifaciens. Journal of Clinical Microbiology 10, 761–765.CrossRefGoogle ScholarPubMed
Penner, J. L. & Hennessy, J. N. (1980). Separate O-grouping schemes for serotyping clinical isolates of Proteus vulgaris and Proteus mirabilis. Journal of Clinical Microbiology 12, 304–309.Google Scholar
Penner, J. L., Hinton, N. A., Duncan, I. B. R., Hennessy, J. N. & Whiteley, G. R. (1979 b). O serotyping of Providencia stuartii isolates collected from twelve hospitals. Journal of Clinical Microbiology 9, 11–14.Google Scholar
Penner, J. L., Hinton, N. A. & Hennessy, J. (1974). Serotyping of Proteus rettgeri on the basis of O antigens. Canadian Journal of Microbiology 20, 777–789.CrossRefGoogle ScholarPubMed
Penner, J. L., Hinton, N. A. & Hennessy, J. (1975). Biotypes of Proteus rettgeri. Journal of Clinical Microbiology 1, 136–142.CrossRefGoogle ScholarPubMed
Penner, J. L., Hinton, N. A.Hennessy, J. N. & Whiteley, G. R. (1976). Reconstitution of the somatic (O-) antigenic scheme for Providencia and preparation of O-typing antisera. Journal of Infectious Diseases 133, 283–292.CrossRefGoogle ScholarPubMed
Penner, J. L., Preston, M. A., Hennessy, J. N., Barton, L. J. & Goodiiody, M. M. (1982). Species differences in susceptibilities of Proteeae spp. to six cephalosporins and three aminoglycosides. Antimicrobial Agents and Chemotherapy 22, 218–221.CrossRefGoogle ScholarPubMed
Phillips, J. E. (1955). In vitro studies of Proteus organisms of animal origin. Journal of Hygiene 53, 26–31.Google Scholar
Rustioian, R. & Stuart, C. A. (1945). The biochemical and serological relationships of the organisms of the genus proteus. Journal of Bacteriology 49, 419–436.Google Scholar
Senior, B. W. (1979). The special affinity of particular types of Proteus mirabilis for the urinary tract. Journal of Medical Microbiology 12, 1–8.CrossRefGoogle ScholarPubMed
Singer, J. & Bar-Chay, J. (1954). Biochemical investigation of Providence strains and their relationship to the Protetts group. Journal of Hygiene 52, 1–8.CrossRefGoogle Scholar
Stickler, D. J., Fawcett, C. & Chawla, J. C. (1985). Providencia stuartii: a search for its natural habitat. Journal of Hospital Infection 6, 221–223.CrossRefGoogle ScholarPubMed
Threlfall, E. J. & Rowe, B. (1984). Antimicrobial drug resistance in salmonellae in Britain.In Antimicrobials and Agriculture (ed. Woodbine, M.), pp. 513–524. London: Butterworth.CrossRefGoogle Scholar
Toni, M., Casewell, M. W. & Schito, G. C. (1980). Reappraisal of the significance of multiply resistant urinary isolates of Proteus rettgeri. Journal of Antimicrobial Chemotherapy 6, 527–534.Google Scholar
Waldhalm, D. G., Hall, R. F., Meinershagen, W. A., Stauber, E. & Frank, F. W. (1974). Combined effect of neonatal calf diarrhoea virus and Providencia stuartii on suckling beef calves. American Journal of Veterinary Research 35, 515–516.Google Scholar
Waldhalm, D. G., Meinershagen, W. A. and Frank, F. W. (1969). Providencia stuartii as an etiologie agent in neonatal diarrhoea in calves. American Journal of Veterinary Research 30, 1573–1575.Google Scholar
Williams, E. W., Hawkey, P.M., Penner, J. L., Senior, B. W. & Barton, L. J. (1983). Serious nosocomial infection caused by Morganella morganii and Proteus mirabilis in a cardiac surgery unit. Journal of Clinical Microbiology 18, 5–9.CrossRefGoogle Scholar