Hostname: page-component-8448b6f56d-cfpbc Total loading time: 0 Render date: 2024-04-24T08:46:09.164Z Has data issue: false hasContentIssue false
  • English
  • Français

The speciation and subtyping of campylobacter isolates from sewage plants and waste water from a connected poultry abattoir using molecular techniques

Published online by Cambridge University Press:  15 May 2009

P. M. F. J. Koenraad ,
R. Ayling ,
W. C. Hazeleger ,
F. M. Rombouts  and
D. G. Newell
P. M. F. J. Koenraad
Affiliation:
Wageningen Agricultural University, Department of Food Science, Bomenweg 2, 6703 HD Wageningen, The Netherlands
R. Ayling
Affiliation:
Central Veterinary Laboratory, Weybridge, New Haw, Addlestone, KT15 3NB, UK
W. C. Hazeleger
Affiliation:
Wageningen Agricultural University, Department of Food Science, Bomenweg 2, 6703 HD Wageningen, The Netherlands
F. M. Rombouts*
Affiliation:
Wageningen Agricultural University, Department of Food Science, Bomenweg 2, 6703 HD Wageningen, The Netherlands
D. G. Newell
Affiliation:
Central Veterinary Laboratory, Weybridge, New Haw, Addlestone, KT15 3NB, UK
*
† Author for correspondence: Professor F. M. Rombouts, Wageningen Agricultural University, Department of Food Science, Bomenweg 2, 6703 HD Wageningen, The Netherlandsw.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In this study the distribution of phenotypes of campylobacter strains in sewage and surface waters was investigated by subtyping and by speciation of isolates from various aquatic environments. These environments included two municipal sewage plants (SPA and SPB) and waste water from a poultry abattoir (WWA). Both the sewage plants SPA and SPB collected domestic and industrial waste, and SPA received drain water from WWA. SPB received no waste water from any meat-processing plant. The isolates were speciated by PCR and subtyped by PCR/RFLP based on the flagellin PCR products.

From all three reservoirs, no Campylobacter lari was isolated, and approximately 80% of the isolates could be identified as C. jejuni and the rest belonged to the C. coli species. The PCR/RFLP typing technique has a high discrimination level and was reproducible between two separate laboratories. The 182 isolates tested yielded 22 distinct Dde I profiles. The results indicate that strains with profiles found in poultry are also detectable in waste water presumed to be solely from domestic and human sources. In addition some strains were unique to the known poultry-related sources, suggesting that avian-specific strains, non-pathogenic to man, may exist in the environment. In contrast some strains were unique to human waste indicating the potential importance of non-poultry sources of infection. No seasonality was observed in the profile distribution. So, at least in the Netherlands, it is unlikely that infections caused by contaminated surface waters contribute to the seasonality of human campylobacteriosis.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 3 , December 1995 , pp. 485 - 494
Copyright
Copyright © Cambridge University Press 1995

References

1.Tauxe, RV. Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations. In: Nachamkin, I, Blaser, MJ, Tompkins, LS, eds. Campylobacter jejuni; current status and future trends. Washington: ASM, 1992; 9–19.Google Scholar
2.Notermans, S, Hoogenboom-Verdegaal A. Existing and emerging foodborne diseases. Int J Food Microbiol 1992; 15: 197–205.CrossRefGoogle ScholarPubMed
3.Taylor, DN, McDermott, KT, Little, JR, Wells, JG, Blaser, MJ. Campylobacter enteritis from untreated water in the Rocky Mountains. Ann Intern Med 1983; 99: 38–40.CrossRefGoogle ScholarPubMed
4.Blaser, MJ, Hardesty, HL, Powers, B, Wang, W-LL. Survival of Campylobacter fetus subsp. jejuni in biological milieus. J Clin Microbiol 1980; 11: 309–13.CrossRefGoogle ScholarPubMed
5.Blaser, MJ, Taylor, DN, Feldman, RA. Epidemiology of Campylobacter infections. In: Butzler, J.-P. ed. Campylobacitr infection in man and animals. Boca Raton: CRC Press Inc., 1984, 143–61.Google Scholar
6.Stelzer, W, Jacob, J, Schulze, E. Environmental aspects of Campylobacter infections. Zentralbl Mikrobiol 1991; 146: 3–15.CrossRefGoogle ScholarPubMed
7.Höller, C. Long-term study of occurrence, distribution and reduction of Campylobacter spp. in the sewage system and waste water treatment plant of a big town. Wat Sci Tech 1988; 20: 529–31.CrossRefGoogle Scholar
8.Jones, K, Betaieb, M, Telford, DR. Correlation between environmental monitoring of thermophilic campylobacters in sewage effluent and the incidence of Campylobacter infection in the community. J Appl Bacteriol 1990; 69: 235–40.CrossRefGoogle ScholarPubMed
9.Koenraad, PMFJ.Hazeleger, WC.Van der Laan, T.Beumer, RR.Rombouts, FM. Survey of Campylobacter spp. in sewage plants in The Netherlands. Food Microbiol 1994; 11: 65–73.CrossRefGoogle Scholar
10.Jacobs-Reitsma, WF.Koenraad, PMFJ.Bolder, NM.Mulder, RWAW. In vitro susceptibility of Campylobacter and Salmonella isolates from broilers to quinolones. ampicillin. tetracyeline. and erythromycin. Vet Quart 1994; 16: 206–8.CrossRefGoogle ScholarPubMed
11.Penner, JL. The genus Campylobacter. Clin Microbiol Rev 1988: 1: 157–72.CrossRefGoogle ScholarPubMed
12.Totten, PA.Patton, CM.Tenover, FC et al. , Prevalence and characterization of hippurate-negative Campylobacter jejuni in King County, Washington. J Clin Microbiol 1987; 25: 1747–52.CrossRefGoogle ScholarPubMed
13.Eyers, M.Chapelle, S.Van Camp, G.Goossens, H.De Wachter, R. Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRXA fragments. J Clin Microbiol 1993: 31: 3340–3.CrossRefGoogle ScholarPubMed
14.Lior, H. New. extended biotyping scheme for Campylobacter jejuni, Campylobacter coli and “Campylobacter laridis”. J Clin Microbiol 1984; 20: 636–40.CrossRefGoogle ScholarPubMed
15.Skirrow, MB.Benjamin, J. Differentiation of enteropathogenic Campylobacter. J Clin Pathol 1980; 33: 1122.CrossRefGoogle ScholarPubMed
16.Lauwers, S.Penner, JL. Serotyping Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. In: Butzler, J-P. ed. Campylobacter infection in man and animals. Boca Raton: CRC Press, Inc., 1984.Google Scholar
17.Lior, H.Woodward, DL.Edgar, JA.Laroche, LJ.Gill, P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol 1982; 15: 761–8.CrossRefGoogle ScholarPubMed
18.Ayling, RD.Woodward, MJ.Evans, S.Newell, DG. A combined PCR/RFLP technique applied to the differentiation of poultry campylobacters for epidemiological investigations. Res Vet Sci. In press.Google Scholar
19.Nachamkin, I.Bohachick, K.Patton, CM. Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol 1993; 31: 1531–6.CrossRefGoogle ScholarPubMed
20.Maniatis, T.Fritsch, EF.Sambrook, J. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory. New York: Cold Spring Harbor. 1982.Google Scholar
21.Hunter, PR. Reproducibility and indices of discriminatory power of microbial typing methods. J Clin Microbiol 1990; 28: 1903–5.CrossRefGoogle ScholarPubMed
22.Van Der Plas, J.Koster, DS.Hofstra, H et al. RFLP-typing of Campylobacter species with universal probes and comparison with serotyping and RAPD. Acta Gastro-Enterologica 1993; 56 (Suppl.): 31.Google Scholar
23.Jacob, J.Bindemann, U.Stelzer, W. Characterization of thermophilic Campylobacters originated from a high-rate sewage treatment plant. Zbl Hyg 1991; 192: 14–24.Google ScholarPubMed
24.Bolton, FJ.Coates, D.Hinchliffe, PM.Robertson, L. Comparison of selective media for isolation of Campylobacter jejuni/coli. J Clin Pathol 1983; 36: 78–83.CrossRefGoogle ScholarPubMed
25.Ng, L-K.Stiles, ME.Taylor, DE. Inhibition of Campylobacter coli and Campylobacter jejuni by antibiotics used in selective growth media. J Clin Microbiol 1985; 22: 510–4.CrossRefGoogle ScholarPubMed
26.Korhonen, LK, Martikainen, PJ. Comparison of the survival of Campylobacter jejuni and Campylobacter coli in culturable form in surface water. Can J Microbiol 1991; 37: 530–3.CrossRefGoogle ScholarPubMed
27.Alm, RA.Guerry, P.Trust, TJ. Distribution and polymorphism of the flagellin genes from isolates of Campylobacter coli and Campylobacter jejuni. J Bacteriol 1993: 175: 3051–7.CrossRefGoogle ScholarPubMed
28.Owen, RJ.Fayos, A.Hernandez, J.Lastovica, A. PCR-based restriction fragment length polymorphism analysis of DNA sequence diversity of flagellin genes of Campylobacter jejuni and allied species. Molec Cell Probes 1993; 7: 471–80.CrossRefGoogle ScholarPubMed
29.Newell, DG.McBride, H.Saunders, F.Dehele, Y.Dolby, JM. The virulence of clinical and environmental isolates of Campylobacter jejuni. J Hyg 1985; 94: 45–54.CrossRefGoogle ScholarPubMed
30.Jacobs-Reitsma, WF.Bolder, NM.Mulder, RWAW. Caecal carriage of Campylobacter and Salmonella in Dutch broiler flocks at slaughter: a one year study. Poult Sci 1994: 73: 1260–6.CrossRefGoogle Scholar