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Serological typing of coagulase-negative staphylococci using monoclonal antibodies

  • C. Gabelish (a1), C. Harbour (a1), M. A. Beard-Pegler (a1), E. Stubbs (a1), R. Steffe (a1), M. Large (a2), A. Vickery (a2) and R. Benn (a2)...

Summary

This investigation was to determine whether monoclonal antibodies (Mabs) could be used to differentiate coagulase-negative staphylococci (C-NS) at species and strain level. Mabs were produced to four Staphylococcus epidermidis strains, two S. haemolyticus strains, one S. saprophyticus strain and one S. warneri strain. A panel of nine antibodies was tested for species and strain specificity against five type strains and 65 clinical isolates of C-NS by enzyme-linked immunosorbent assay (ELISA). Species specificity was found with Mab D150 produced to one S. haemolyticus strain. Using Mab D150 and Mab D198 in conjunction, identification of 90% of S. haemolyticus isolates to species level was achieved. S. saprophyticus Mab K84 reacted with most other strains of C-NS tested but only three S. haemolyticus strains (16%). This finding provides further evidence that S. haemolyticus possesses different surface determinants to other C-NS which could form the basis of a typing scheme for S. haemolyticus using Mabs D150, D198 and K84.

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Copyright

Corresponding author

Correspondence: Dr C. Harbour, Department of Infectious Diseases, University of Sydney, NSW 2006, Australia.

References

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Serological typing of coagulase-negative staphylococci using monoclonal antibodies

  • C. Gabelish (a1), C. Harbour (a1), M. A. Beard-Pegler (a1), E. Stubbs (a1), R. Steffe (a1), M. Large (a2), A. Vickery (a2) and R. Benn (a2)...

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