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Serological typing of coagulase-negative staphylococci using monoclonal antibodies

Published online by Cambridge University Press:  15 May 2009

C. Gabelish ,
C. Harbour ,
M. A. Beard-Pegler ,
E. Stubbs ,
R. Steffe ,
M. Large ,
A. Vickery  and
R. Benn
C. Gabelish
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
C. Harbour*
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
M. A. Beard-Pegler
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
E. Stubbs
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
R. Steffe
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
M. Large
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
A. Vickery
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
R. Benn
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
*
Correspondence: Dr C. Harbour, Department of Infectious Diseases, University of Sydney, NSW 2006, Australia.
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Summary

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This investigation was to determine whether monoclonal antibodies (Mabs) could be used to differentiate coagulase-negative staphylococci (C-NS) at species and strain level. Mabs were produced to four Staphylococcus epidermidis strains, two S. haemolyticus strains, one S. saprophyticus strain and one S. warneri strain. A panel of nine antibodies was tested for species and strain specificity against five type strains and 65 clinical isolates of C-NS by enzyme-linked immunosorbent assay (ELISA). Species specificity was found with Mab D150 produced to one S. haemolyticus strain. Using Mab D150 and Mab D198 in conjunction, identification of 90% of S. haemolyticus isolates to species level was achieved. S. saprophyticus Mab K84 reacted with most other strains of C-NS tested but only three S. haemolyticus strains (16%). This finding provides further evidence that S. haemolyticus possesses different surface determinants to other C-NS which could form the basis of a typing scheme for S. haemolyticus using Mabs D150, D198 and K84.

Type
Special Article
Information
Epidemiology & Infection , Volume 106 , Issue 2 , April 1991 , pp. 231 - 237
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Younger, JJ, Christensen, GD, Bartley, DL, Simmons, JCH, Barrett, FE. Coagulase-negative staphylococci isolated from cerebrospinal fluid shunts: importance of slime production, species identification, and shunt removal to clinical outcome. J Infect Dis 1987; 156: 548–54.CrossRefGoogle ScholarPubMed
2.Etienne, J, Brun, Y, El Solh, N et al. , Characterization of clinically significant isolates of Staphylococcus epidermidis from patients with endocarditis. J Clinic Microbiol 1988; 26: 613–7.CrossRefGoogle ScholarPubMed
3.Beard-Pegler, MA, Gabelish, CL, Stubbs, E et al. , Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients. Epidemiol Infect 1989; 102: 365–78.CrossRefGoogle ScholarPubMed
4.Pillet, J, Orta, B. Analyse sérologique des staphylocoques coagulase negatifs: étude des agglutiogènes caracteristiques des souches types des neuf espèces indivualisées par Schleifer et Kloos. Ann Microbiol (Institut Pasteur) 1977; 128B: 475–86.Google Scholar
5.Gabelish, C, Steffe, R, Harbour, C, Stubbs, E, Beard-Pegler, M, Vickery, A. Species differentiation of coagulase-negative staphylococci using monoclonal antibodies. In: Jeljaszewicz, J, ed. Staphylococci and staphylococcal infections. Stuttgart: Gustav Fisher Verlag, 1990. In press.Google Scholar
6.Ohshima, Y, Ohtomo, T, Ichiman, Y, Chomarat, M, Yoshida, K. Comparison of cell wall teichoic acid fractions isolated from three different encapsulated strains of Staphylococcus epidermidis. Ann Microbiol (Institut Pasteur) 1984; 135A: 353–65.CrossRefGoogle Scholar
7.Harbour, C, Fletcher, A. Hybridomas: production and selection. In: Butler, M, ed. Mammalian cell culture and technology: a practical approach. Oxford: Oxford University Press. In press.Google Scholar
8.Pillet, J, Orta, B. Species and serotypes in coagulase-negative staphylococci. In: Jeljaszewicz, J, ed. Staphylococci and staphylococcal infections. Stuttgart: Gustav Fischer Verlag, 1981: 147–52.Google Scholar
9.Fleurette, J, Brun, Y, Bes, M, Coulet, M, Forey, F. Infections caused by coagulase-negative staphylococci other than S. epidermidis and S. saprophyticus. Zentralbl Bakteriol Mikrobiol Hyg 1987; Abt 1 (suppl 16): 195–208.Google Scholar
10.Schleifer, KH, Meyer, SA, Rupprecht, M. Relatedness among coagulase-negative staphylococci: deoxyribonucleic acid reassociation and comparative immunological studies. Arch Microbiol 1979; 122: 93–101.CrossRefGoogle ScholarPubMed