Hostname: page-component-848d4c4894-cjp7w Total loading time: 0 Render date: 2024-06-25T20:17:48.141Z Has data issue: false hasContentIssue false
  • English
  • Français

The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests

Published online by Cambridge University Press:  25 March 2010

R. J. Chappel ,
J. Hayes ,
B. A. Rogerson  and
L. J. Shenfield
R. J. Chappel
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
J. Hayes
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
B. A. Rogerson
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
L. J. Shenfield
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Serum samples were obtained from 281 heifers vaccinated with Brucella abortus strain 19, and from 50 heifers that had received two injections of killed B. abortus strain 45/20 adjuvant (K45/20A) vaccine. The serological response measured by the brucellosis radioimmunoassay (RIA) was compared with responses measured by other tests.

The serological responses of cattle during the first weeks after strain 19 vaccination were found to give little guide to the frequency of persistent reactions.

In the case of strain 19, persistent reactions were considered to be those occurring 12 or more months after vaccination. In heifers vaccinated at the recommended age, small numbers of persistent reactions were given by the RIA (four in 374 sera), the complement fixation test using warm fixation (CFTW) (six in 383) and cold fixation (one in 185), the serum agglutination test (two in 222) and the indirect haemolysis test (IHLT) (two in 369). The Rose Bengal plate test gave 74 persistent reactions in 374 sera.

Five of the 50 heifers gave particularly prolonged responses to K45/20A vaccine. In these animals the RIA and IHLT remained positive for longer than the CFTW.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 1 , February 1982 , pp. 11 - 19
Copyright
Copyright © Cambridge University Press 1982

References

Allan, G. S., Chappel, R. J., Williamson, P. & McNaught, D. J. (1976). A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes. Journal of Hygiene 76, 287.CrossRefGoogle ScholarPubMed
Alton, G. G. (1978) Recent developments in vaccination against bovine brucellosis. Australian Veterinary Journal 54, 551.CrossRefGoogle ScholarPubMed
Alton, G. G., Jones, L. M. & Pietz, D. E. (1975). Laboratory techniques in brucellosis. 2nd ed.World Health Organization Monograph Series no. 55. Geneva.Google Scholar
Anon, (1977). Standardised complement fixation test for bovine brucellosis. Australian Veterinary Journal 53, 394.CrossRefGoogle Scholar
Beh, K. J. (1974) Quantitative distribution of Brucella antibody amongst immunoglobulin classes in vaccinated and infected cattle. Research in Veterinary Science 17, 1.CrossRefGoogle ScholarPubMed
Beh, K. J. (1975). Immunoglobulin class specificity of non-agglutinating antibody produced in cattle following Brucella abortus 45/20 vaccination. Australian Veterinary Journal 51, 481.CrossRefGoogle ScholarPubMed
Chappel, R. J., Hayes, J., Brain, G. J. & McNaught, D. J. (1981). A modified radioimmunoassay for antibodies against Brucella abortus. Journal of Hygiene 88, 1.CrossRefGoogle Scholar
Chappel, R. J., Williamson, P., McNaught, D. J., Dalling, M. J. & Allan, G. S. (1976). Radioimmunoassay for antibodies against Brucella abortus, a new serological test for bovine brucellosis. Journal of Hygiene 77, 369.CrossRefGoogle ScholarPubMed
Corbel, M. J. (1972). Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis. Journal of Hygiene 70, 779.Google ScholarPubMed
Corbel, M. J. (1976). The immune response to Brucella abortus 45/20 adjuvant vaccine in terms of immunoglobulin class. Developments in Biological Standardization 31, 141.Google Scholar
Corden, D. O. & Carter, M. E. (1979). Persistence of Brucella abortus infection in six herds of cattle under brucellosis eradication. New Zealand l'eterinary Journal 27, 255.CrossRefGoogle Scholar
Cunningham, B. & O'Connor, M. (1971). The use of killed 45/20 adjuvant vaccine as a diagnostic agent in the final stages of the eradication of brucellosis: the clearance of brucellosis from problem herds by interpretation of anamnestic serological responses. Veterinary Record 89, 680.CrossRefGoogle ScholarPubMed
Diaz, R. & Jones, L. M. (1973) The immuno diffusion method for the identification of cattle vaccinated with Brucella abortus strain 45/20. Veterinary Record 93, 300.CrossRefGoogle ScholarPubMed
Elberg, S. S. (1973). Immunity to Brucella infection. Medicine, Baltimore 52, 339.CrossRefGoogle ScholarPubMed
Hayes, J. & Chappel, R. J. (1981). A comparison of the results of the brucellosis radioimmunoassay and other serological tests in experimentally infected cattle. Journal of Hygiene 88, 21.CrossRefGoogle Scholar
Lambert, G., Amerault, T. E., Manthei, C. A. & Goode, E. R. (1961). Immunogenic response of calves vaccinated at different ages with Brucella abortus strain 19. Proceedings of the United States Livestock Sanitation Association 65, 93.Google Scholar
Meyer, M. E. & Nelson, C. J. (1969). Persistence of Brucella abortus strain 19 infection in immunized cattle. Proceedings of the United States Animal Health Association 73, 159.Google Scholar
Morse, E. V., Schneider, D. W. & McNutt, S. H. (1955). The effect of incubation at 56 C on the tube agglutination test for bovine brucellosis. American Journal of Veterinary Research 16, 269.Google ScholarPubMed
Patterson, J. M., Deyoe, B. L. & Stone, S. S. (1976). Identification of immunoglobulins associated with complement fixation, agglutination, and low pH buffered antigen teats for brucellosis. American Journal of Veterinary Research 37, 319.Google Scholar
Plackett, P., Alton, G. G., Carter, P. D. & Corner, L. A. (1980). The indirect haemolysis test (IHLT) for bovine brucellosis–comparisons with the complement fixation test (CFT) in vaccinated and experimentally infected cattle. Australian Veterinary Journal 56, 405.CrossRefGoogle ScholarPubMed
Plackett, P., Cottew, G. S. & Best, S. J. (1976). An indirect haemolysis test (IHLT) for bovine brucellosis. Australian Veterinary Journal 52, 136.CrossRefGoogle Scholar
Reid, M. A. & Harvey, P. R. (1972). The use of Brucella abortus 45/20 adjuvant vaccine as a diagnostic aid in the brucellosis eradication campaign in Papua New Guinea. Australian Veterinary Journal 48, 495.CrossRefGoogle ScholarPubMed
Rose, J. E., Lambert, G. & Roepke, M. H. (1964). Ultracentrifugation and heat-inactivation studies on seroagglutinins of pregnant heifers artificially infected with virulent Brucella abortus. American Journal of Veterinary Research 25, 329.Google ScholarPubMed
Rose, J. E. & Roepke, M. H. (1964). Physicochemical studies on post-vaccinal Brucella agglutinins in bovine serum. American Journal of Veterinary Research 25, 325.Google Scholar