Hostname: page-component-7479d7b7d-qlrfm Total loading time: 0 Render date: 2024-07-11T03:03:15.807Z Has data issue: false hasContentIssue false
  • English
  • Français

Rubella antibody measured by radial haemolysis. Characteristics and performance of a simple screening method for use in diagnostic laboratories*

Published online by Cambridge University Press:  15 May 2009

J. B. Kurtz ,
P. P. Mortimer ,
P. R. Mortimer ,
P. Morgan-Capner ,
M. S. Shafi  and
G. B. B. White
J. B. Kurtz
Affiliation:
Virology Laboratory, Churchill Hospital, Oxford 0X3 7LJ
P. P. Mortimer
Affiliation:
Central Public Health Laboratory, Colindale Avenue, London NW9 5HT
P. R. Mortimer
Affiliation:
Public Health Laboratory, Coventry and Warwickshire Hospital, Coventry CV1 4FH
P. Morgan-Capner
Affiliation:
Department of Medical Microbiology, Kings College Hospital Medical School, London SE5 8RX
M. S. Shafi
Affiliation:
Public Health Laboratory, Central Middlesex Hospital, Park Royal, London, NW 1O 7NS
G. B. B. White
Affiliation:
Public Health Laboratory, Fazakerley Hospital, Liverpool L9 7AL
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A simple method for preparing radial haemolysis gels for rubella antibody screening is described. In use it gave clear zones of haemolysis when a standard serum was tested at dilutions down to 5·6 i.u./ml rubella antibody. In five lab oratories 8404 sera were screened by the method and the results were read by comparing zones of haemolysis with that of a standard serum diluted to contain 15 i.u./ml antibody. A zone ⋝ 15 i.u./ml, indicating immunity, was given by 7433 (88·4%) of the sera. No zone indicating susceptibility was seen with 748 (8·9%) sera. Small zones, < 15 i.u./ml standard, were given by 189 (2·2%) sera, and in only 34 cases (0·4%) did non-specific haemolysis interfere with the test readings. Further testing of the radial haemolysis negative and low positive sera by the haemagglutination inhibition test gave rise to some discrepant results which are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 84 , Issue 2 , April 1980 , pp. 213 - 222
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Bradstreet, C. M. P., Kirkwood, B., Pattison, J. R. & Tonrn, J. O'H. (1978). The derivation of a minimum immune titre of rubella haemagglutinin-iuhibition (HI) antibody. A Public Health Laboratory Service collaborative survey. Journal of Hygiene 81, 383–8.CrossRefGoogle ScholarPubMed
Bruce, White C. B. & Tinnion, K. H. (1975). A non-specific inhibitor of rubella haem-agglutination. Lancet ii, 664.CrossRefGoogle Scholar
Grillner, L. & Blomberg, J. (1976). Haemolysis-In-Gel and Neutralization Tests for Determination of Antibodies to Mumps Virus. Journal of Clinical Microbiology 4, 11–15.CrossRefGoogle Scholar
Public Health Laboratory Service Standing Anvisory Committee on Viral Reagents (1978). Haemagglutination-inhibition test for the detection of rubella antibody. Journal of Hygiene 81, 373–82.CrossRefGoogle Scholar
Russell, S. M., McCahon, D. & Beare, A. S. (1975). A single Radial Haemolysis Technique for the Measurement of Influenza Antibody. Journal of General Virology 27, 1–10.CrossRefGoogle ScholarPubMed
Skaug, K., Ørstavik, I. & Ulstrup, J. C. (1975). Application of the Passive Haemolysis Test for the Determination of Rubella Virus Antibodies. Acta Pathologica et Microbiologica Sandinavica 83, 367–72.Google ScholarPubMed
Strannegard, O., Grillner, L. & Lindberg, I. M. (1975). Haemolysis-in-gel Test for the Demonstration of Antibodies to Rubella Virus. Journal of Clinical Microbiology 1, 491–4.CrossRefGoogle Scholar
Wesslen, L. (1978). Demonstration of Antibodies to Measles Virus by Haemolysis-in-gel (HIG) Test. Scandinavian Journal of Infectious Diseases 10, 15–20.CrossRefGoogle Scholar