Skip to main content Accessibility help
×
Home

Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients

  • M. A. Beard-Pegler (a1), C. L. Gabelish (a1), E. Stubbs (a1), C. Harbour (a1), J. Robson (a2), M. Falk (a2), R. Benn (a2) and A. Vickery (a2)...

Summary

The predominance of coagulase-negative staphylococci as normal skin flora is thought to be a factor in their association with episodes of peritonitis in patients undergoing continuous ambulatory peritoneal dialysis. We investigated the prevalence of peritonitis-associated strains on the skin of 28 patients undergoing peritoneal dialysis. Coagulase-negative staphylococci were the most frequently isolated organisms. comprising 47% of peritoncal dialysis fluid isolates and 59% of body site isolates. A total of 142 coagulase-negative staphylococci were speciated. tested for their antimicrobial sensitivity and slime production. and identified by phage typing and plasmid-profile analysis. Staphylococcus epidermidis was the most commonly identified species from both peritoncal dialysis fluid (73%) and body sites (53%). Multiple antibiotic resistance was common, and the greater proportion of isolates were resistant to methicillin: 63·6% of peritoncal dialysis fluid isolates and 61·7% of body-site isolates.isolates. S. haemolyticus isolates were significantly more resistant to methicillin than other species. By phage typing and plasmid-profile analysis it was shown that peritonitis was rarely caused by skincolonizing strains. In only 3 of 14 patients were peritonitis-associated strains isolated as skin colonizers, and no patients developed peritonitis due to organisms previously isolated as skin colonizers.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients
      Available formats
      ×

Copyright

References

Hide All
Archer, G. L.. Karchmer, A. W.. Vishniavsky, N. & Johnston, J. L. (1984). Plasmidpattern analysis for the differentiation of infecting from noninfecting Staphylococcus epidermidis. The Journal of Infectious Diseases 149, 913920.
Baddour, L. M.. Smalley, D. L.. Kraus, A. P. Jr. Lamoreaux, W. J.. & Christensen, G. D.. (1986). Comparison of microbiologic characteristics of pathogenic and saprophytic coagulasenegative staphylococci from patients on continuous ambulatory peritoneal dialysis. Diagnostic Microbiology and Infectious Diseases 5, 197205.
Blair, J. E. & Williams, R. E. O. (1961). Phage typing of staphylococci. Bulletin of the world Health Organisation 24, 771784.
Christensen, G. D.Simpson, W. A., Bisno, A. L. & Beachey, E. H. (1982). Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infection and Immunity 37, 318326.
Deighton, M. A., Franklin, J. C., Spicer, W. J. & Balkau, B. (1988). Species identification. antibiotic sensitivity and slime production of coagulase-negative staphylococci isolated from clinical specimens. Epidemiology and Infection 101, 99113.
Eisenberg, E. S., Ambalu, M., Szylagi, G., Aning, V. & Soeiro, R. (1987). Colonization of skin and development of peritonitis due to coagulase-negative staphylococci in patients undergoing peritoneal dialysis. The Journal of Infectious Diseases 156, 478482.
Etienne, J., Brun, Y.El Solh, N., Delorme, V., Mouren, C., Bes, M. & Fleurette, J. (1988). Characterization of clinically significant isolates of Staphylococcus epidermidis from patients with endocarditis. Journal of Clinical Microbiology 26, 613617.
Gill, V. J., Selepak, S. T. & Williams, E. C. (1983). Species identification and antibiotic susceptibilities of coagulase-negative staphylococci isolated from clinical specimens. Journal of Clinical Microbiology 18, 13141319.
Hamilton-Miller, J. M. T. & Iliffe, A. (1985). Antimicrobial resistance in coagulase-negative staphylococci. Journal of Medical Microbiology 19, 217226.
Jaffe, H. W., Sweeney, H. M., Weinstein, R. A., Kabins, S. A., Nathan, C. & Cohen, S. (1982). Structural and phenotypic varieties of gentamicin resistance plasmids in hospital strains of Staphylococcus aureus and coagulase-negative staphylococci Antimicrobial Agents and Chemotherapy 21, 773779.
Lyon, B. R., May, J. W. & Skurrey, R. A. (1984). Tn4001: a gentamicin and kanomycin resistance transposon in Staphylococcus aureus. Molecular and General Genetics 193, 554–6.
Mickelsen, P. A., Plorde, J. J., Gordon, K. P., Hargiss, C., McClure, J., Schoenknecht, F. D., Condie, F., Tenover, F. C. & Tompkins, L. S. (1985). Instability of antibiotic resistance in a strain of Staphylococcus epidermidis isolated from an outbreak of prosthetic valve endocarditis. The Journal of Infectious Diseases 152, 5058.
Parisi, J. T. (1985). Coagulase-negative staphylococci and the epidemiological typing of Staphylococcus epidermidis. Microbiological Reviews 49, 126139.
Parisi, J. T., Lampson, B.C., Hoover, D.L. & Khan, J.A. (1986). Comparison of epidemiologic markers for Staphylococcus epidermidis. Journal of Clinical Microbiology 24, 5660.
Peters, G., Schumacher-Perdreau, F., Jansen, B., Bey, M. & Pulverer, G. (1987). Biology of S. epidermidis extracellular slime. Zentralblatt für Bakteriologie. Mikrobiologie and Hygiene. 1, Abteilung. (Suppl. 16). 1532.
Reuther, J. W. A. (1986). A simplified system for the identification of staphylococci by multipoint inoculation of test media. Journal of Medical Microbiology 22, 179182.
Richardson, J.F. & Marples, R.R. (1987). Phage typability of coagulase-negative staphylococci in relation to antibiotic resistance. Zentralblatt für Bakteriologie. Mikrobiologie and Hygiene. 1, Abteilung. (Suppl. 16). 235246.
de Saxe, M. J. & Notley, C. M. (1978). Experiences with the typing of coagulase-negative staphylococci and micrococci. Zentralblatt für Bakteriologie. Mikrobiologie and Hygiene,241, 4659.
Takahashi, S. & Nagano, Y. (1984). Rapid procedure for isolation of plasmic DNA and application to epidemiological analysis. Journal of Clinical Microbiology 20, 608613.
Tennent, J. M., May, J. W. & Skurray, R. A. (1984). Multiple antibiotic resistance in Staphylococcus aureus and Staphylococcus epidermidis: plasmids in strains associated with nosocomial infection. Pathology 16, 250255.
Washington, J. A. II & Sutter, V. L. (1980). The dilution susceptibility test: Agar and macrobroth dilution procedures. In Manual of Clinical Microbiology (ed. Lenette, E. H., Balows, A., Hausler, W. J. and Truant, P. J.) 3rd ed. pp. 453458. Washington. D.C.: American Society for Microbiology.
West, T. E.. Walshe, J. J.. Krol, C. P. & Amsterdam, D. (1986). Staphylococcal peritonitis in patients on continuous peritoneal dialysis. Journal of Clinical Microbiology 23, 809812.
Younger, J. J.. Christensen, G. D.. Bartley, D. L.. Simmons, J. C. H. & Barrett, F. F. (1987). Coagulase-negative staphylococci isolated from cerebrospinal fluid shunts: importance of slime production. species identification, and shunt removal to clinical outcome. The Journal of Infectious Diseases 156, 548554.

Related content

Powered by UNSILO

Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients

  • M. A. Beard-Pegler (a1), C. L. Gabelish (a1), E. Stubbs (a1), C. Harbour (a1), J. Robson (a2), M. Falk (a2), R. Benn (a2) and A. Vickery (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.