Skip to main content Accessibility help
×
×
Home

The presence of HBV mRNA in the fertilized in vitro embryo of HBV patients confirms vertical transmission of HBV via the ovum

  • F. YE (a1), Y. JIN (a1), Y. KONG (a1), J. Z. SHI (a2), H. T. QIU (a2), X. ZHANG (a1), S. L. ZHANG (a1) and S. M. LIN (a1)...

Summary

This study aimed to confirm that vertical transmission of hepatitis B virus (HBV) can occur via the infected ovum. Specimens studied were obtained from discarded test-tube embryos from mothers with chronic HBV infection who had received in vitro fertilization treatment. Single-cell reverse transcriptase–polymerase chain reaction was used to detect HBV mRNA in the embryos. HBV mRNA was detected in the cleavage embryos of patients with chronic HBV infection, with a detection rate of 13·2% (5/38). The level of serum HBV DNA was not related to the HBV mRNA positivity rates in embryos. In this study, HBV mRNA was detected in test-tube embryos from HBV-infected mothers who had received in vitro fertilization treatment. This confirms the theory of vertical transmission of HBV via the ovum, thereby providing an important theoretical basis for further study on the mechanism of HBV vertical transmission, influencing factors and blocking measures.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      The presence of HBV mRNA in the fertilized in vitro embryo of HBV patients confirms vertical transmission of HBV via the ovum
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      The presence of HBV mRNA in the fertilized in vitro embryo of HBV patients confirms vertical transmission of HBV via the ovum
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      The presence of HBV mRNA in the fertilized in vitro embryo of HBV patients confirms vertical transmission of HBV via the ovum
      Available formats
      ×

Copyright

Corresponding author

*Author for correspondence: Dr S. M. Lin, Departments of Infectious Disease and Gynecology and Obstetrics, The First Hospital of Xi'an Jiaotong University, Jiankang Road, 1#, Xi'an 710061, Shanxi Province, China. (Email: linshumei123@126.com)

References

Hide All
1.Zhou, SL, Zhao, LS, Li, F. HBV DNA was detected in ovum of the patients with chronic hepatitis B virus infection. Journal of Heredity Disease 1989; 6: 4648.
2.Kathryne, MK, et al. Laser capture microdissection and single-cell RT-PCR without RNA purification. Journal of Immunological Methods 2005; 302: 9098.
3.Liu, B, et al. Single cell RT-PCR demonstrates differential expression of GABAC receptor U subunits in rat hippocampal pyramidal and granule cells. Molecular Brain Research 2004; 123: 16.
4.Barone, M, et al. Gene expression analysis in HBV transgenic mouse liver: a model to study early events related to hepatocarcinogenesis. Molecular Medicine 2006; 12: 115123.
5.Cabrerizo, M, et al. Molecular analysis of hepatitis B virus DNA in serum and peripheral blood mononuclear cells from hepatitis B surface antigen-negative cases. Hepatology 2000; 32: 116123.
6.Chan, AW, et al. Transgenic cattle produced by reverse transcribed gene transfer in oocytes. Proceedings of the National Academy of Sciences USA 1998; 95: 1402814033.
7.Wang, C. The construction of HBV DNA oocyte vector and the detection of HBV DNA in the hepatitis B patients' ovaries. Qing Dao University, 2006.
8.Anne, SM. Improving gene transfer into live tock. Science 1998; 282: 16191620.
9.Bagis, H, Arat, S, Merean, HO. Stable transmission and expression of the hepatitis B virus total genome in hybrid transgenie mice until F10 generation. Journal of Experimental Zoology, Part A: Comparative Experimental Biology 2006; 305: 420427.
10.Chen, GL, et al. Replication and expression of oocyte-mediated HBV DNA in early mouse embryo. Carcinogenesis, Teratogenesis & Mutagenesis 2005; 17: 179182.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed