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Nasal immunoglobulin responses in acute rubella determined by the immunofluorescent technique

Published online by Cambridge University Press:  15 May 2009

J. E. Cradock-Watson ,
Margaret K. S. Ridehalgh ,
M. S. Bourne  and
Eltse M. Vandervelde
J. E. Cradock-Watson
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
Margaret K. S. Ridehalgh
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
M. S. Bourne
Affiliation:
University of Salford Health Service
Eltse M. Vandervelde
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, LondonNW9 5HT
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The indirect immunofluorescent technique has been used to study the specific immunoglobulin responses in nasal secretions from ten adults with acute rubella. Titres of IgA antibody in nasal washings usually exceeded those of IgG, but both types of antibody were detected in all patients. They appeared a few days after the rash, reached maximum titres during the second week and then declined. IgA antibody was no longer detectable after 47 days and was not detected at all in nasal washings from adults who had experienced rubella in the past. Low titres of IgG antibody persisted in some patients for longer than IgA and traces of IgG were found in nasal washings from a minority of adults with a past history of rubella. Nasal antibodies in acute rubella are therefore transient and unlikely to take part in resistance to reinfection.

In sucrose-density gradients nasal IgA antibody sedimented more rapidly than IgG and there was little overlap between these two types of antibody. IgA antibody in serum was more heterogeneous; it was found in nearly all the fractions which contained IgG antibody and in many of those which contained IgM.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 3 , September 1973 , pp. 603 - 617
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Alford, R. H., Rossen, R. D., Butler, W. T. & Kasel, J. A. (1967). Neutralizing and hemagglutination-inhibiting activity of nasal secretions following experimental human infection with A2 influenza virus. Journal of Immunology 98, 724.CrossRefGoogle ScholarPubMed
Artenstein, M. S., Bellanti, J. A. & Buescher, E. L. (1964). Identification of the antiviral substances in nasal secretions. Proceedings of the Society for Experimental Biology and Medicine 117, 558.CrossRefGoogle ScholarPubMed
Bellanti, J. A., Artenstein, M. S. & Buescher, E. L. (1905). Characterization of virus neutralizing antibodies in human serum and nasal secretions. Journal of Immunology 94, 344.CrossRefGoogle Scholar
Bürgin-Wolf, A., Hernandez, R. & Just, M. (1971). Separation of rubella IgM, IgA and IgG antibodies by gel filtration on agarose. Lancet ii, 1278.CrossRefGoogle Scholar
Butler, W. T., Rossen, R. D. & Waldmann, T. A. (1967). The mechanism of appearance of immunoglobulin A in nasal secretions in man. Journal of Clinical Investigation 46, 1883.CrossRefGoogle ScholarPubMed
Cate, T. R., Rossen, R. D., Douglas, R. G. Jr., Butler, W. R. & Couch, R. B. (1966). The role of nasal secretion and serum antibody in the rhinovirus common cold. American Journal of Epidemiology 84, 352.CrossRefGoogle ScholarPubMed
Cradock-Watson, J. E., Bourne, M. S. & Vandervelde, E. M. (1972). IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique. Journal of Hygiene 70, 473.CrossRefGoogle ScholarPubMed
Hobbs, J. R. (1970). Immune globulins in some diseases. British Journal of Hospital Medicine 3, 669.Google Scholar
Ogra, P. L. & Karzon, D. T. (1971). Formation and function of poliovirus antibody in different tissues. Progress in Medical Virology 13, 156.Google Scholar
Ogra, P. L., Kerr-Grant, D., Umana, G., Dzierba, J. & Weintraub, D. (1971). Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus. New England Journal of Medicine 285, 1333.CrossRefGoogle ScholarPubMed
Perkins, J. C., Tucker, D. N., Knopf, H. L. S., Wenzel, R. P., Kapikian, A. Z. & Chanock, R. M. (1969). Comparison of protective effect of neutralizing antibody in serum and nasal secretions in experimental rhinovirus type 13 illness. American Journal of Epidemiology 90, 519.CrossRefGoogle ScholarPubMed
Rossen, R. D., Douglas, R. G. Jr., Cate, T. R., Couch, R. B. & Butler, W. T. (1966). The sedimentation behaviour of rhinovirus neutralizing activity in nasal secretion and serum following the rhinovirus common cold. Journal of Immunology 97, 532.CrossRefGoogle ScholarPubMed
Rossen, R. D., Mobgan, C., Hsu, K. C., Butler, W. T. & Rose, H. M. (1967). Localization of 11S external secretory IgA by immunofluorescence in tissues lining oral and respiratory passages in man. Journal of Immunology 100, 706.CrossRefGoogle Scholar
Rygaard, J. & Olsen, W. (1969). Interference filters for improved immunofluorescence microscopy. Acta Pathologica et Microbiologica Scandinavica 76, 146.CrossRefGoogle ScholarPubMed
Smith, C. B., Purcell, R. H., Bellanti, J. A. & Chanock, R. M. (1966). Protective effect of antibody to parainfluenza type 1 virus. New England Journal of Medicine 275, 1145.CrossRefGoogle ScholarPubMed
Thompson, K. M. & Tobin, J. O'H. (1970). Isolation of rubella virus from abortion material. British Medical Journal ii, 264.CrossRefGoogle Scholar
Tomasi, T. B. (1972). Secretory immunoglobulins. New England Journal of Medicine 287, 500.CrossRefGoogle Scholar
Tourville, D. R., Adler, R. H., Bienenstock, J. & Tomasi, T. B. (1969). The human secretory immunoglobulin system: immunohistological localization of A, secretory ‘piece’, and lactoferrin in normal human tissues. Journal of Experimental Medicine 129, 411.CrossRefGoogle ScholarPubMed