Skip to main content Accessibility help
×
Home

Molecular typing of Leptospira spp. strains isolated from field mice confirms a link to human leptospirosis

  • S. J. LI (a1), D. M. WANG (a1), C. C. ZHANG (a2), X. W. LI (a2), H. M. YANG (a2), K. C. TIAN (a1), X. Y. WEI (a1), Y. LIU (a1), G. P. TANG (a1), X. G. JIANG (a2) and J. YAN (a3)...

Summary

In recent years, human leptospirosis has been reported in Jinping and Liping counties, Guizhou province, but the leptospires have never been isolated. To track the source of infection and understand the aetiological characteristics, we performed surveillance for field mice carriage of leptospirosis in 2011. Four strains of leptospire were isolated from Apodemus agrarius. PCR confirmed the four isolates as pathogenic. Multiple-locus variable-number tandem repeat analysis (MLVA) showed that the four strains were closely related to serovar Lai strain 56601 belonging to serogroup Icterohaemorrhagiae, which is consistent with the antibody detection results from local patients. Furthermore, the diversity of leptospiral isolates from different hosts and regions was demonstrated with MLVA. Our results suggest that A. agrarius may be the main carrier of Leptospira in Jinping and Liping counties, and the serogroup Icterohaemorrhagiae serovar may be the epidemic serogroup of Leptospira. This will contribute to the control and prevention of leptospirosis in these localities.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Molecular typing of Leptospira spp. strains isolated from field mice confirms a link to human leptospirosis
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Molecular typing of Leptospira spp. strains isolated from field mice confirms a link to human leptospirosis
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Molecular typing of Leptospira spp. strains isolated from field mice confirms a link to human leptospirosis
      Available formats
      ×

Copyright

Corresponding author

*Author for correspondence: Dr S. J. Li, Institute of Communicable Disease Control and Prevention, Guizhou Provincial Centre for Disease Control and Prevention, 72 Bageyan Road, Guiyang 550004, Guizhou, P.R. China. (Email: zjumedjun@163.com) [S. J. Li] (Email: med_bp@zju.edu.cn) [J. Yan]

References

Hide All
1.Levett, PN. Leptospirosis. Clinical Microbiology Reviews 2001; 14: 296326.
2.Vijayachari, P, Sugunan, AP, Shriram, AN. Leptospirosis: an emerging global public health problem. Journal of Biosciences 2008; 33: 557569.
3.Bezerra da Silva, J, et al. Evaluation of the use of selective PCR amplification of LPS biosynthesis genes for molecular typing of Leptospira at the serovar level. Current Microbiology 2011; 62: 518525.
4.Gouveia, EL, et al. Leptospirosis-associated severe pulmonary hemorrhagic syndrome, Salvador, Brazil. Emerging Infectious Diseases 2008; 14: 505508.
5.McBride, AJ, et al. Leptospirosis. Current Opinion in Infectious Diseases 2005; 18: 376386.
6.Guerra, MA. Leptospirosis. Journal of the American Veterinary Medical Association 2009; 234: 472478.
7.Meerburg, BG, Singleton, GR, Kijlstra, A. Rodent-borne diseases and their risks for public health. Critical Reviews in Microbiology 2009; 35: 221270.
8.Koizumi, N, et al. Investigation of reservoir animals of Leptospira in the northern part of Miyazaki Prefecture. Japanese Journal of Infectious Diseases 2008; 61: 465468.
9.Vinetz, JM. Leptospirosis. Current Opinion in Infectious Diseases 2001; 14: 527538.
10.Plank, R, Dean, D. Overview of the epidemiology, microbiology, and pathogenesis of Leptospira spp. in humans. Microbes and Infection 2000; 2: 12651276.
11.Faine, S. Leptospira and Leptospirosis. Melbourne: MedSci, 1999.
12.Mayer-Scholl, A, et al. Comparison of two PCR systems for the rapid detection of Leptospira spp. from kidney tissue. Current Microbiology 2011; 62: 11041106.
13.Yang, K, et al. Epidemiology of leptospirosis in Liping County, Guizhou, 2001–2008. Disease Surveillance 2009; 24: 768769.
14.Levett, PN. Leptospirosis. Clinical Microbiology Reviews 2001; 14: 296326.
15.Morey, RE, et al. Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing. Journal of Clinical Microbiology 2006; 44: 35103516.
16.Majed, Z, et al. Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto. Journal of Clinical Microbiology 2005; 43: 539545.
17.Slack, AT, et al. Development of a multiple-locus variable number of tandem repeat analysis (MLVA) for Leptospira interrrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia. Annals of Clinical Microbiology and Antimicrobials 2005; 4: 10.
18.Slack, A, et al. An improved multiple-locus variable number of tandem repeats analysis for Leptospira interrrogans serovar Australis: a comparison with fluorescent amplified fragment length polymorphism analysis and its use to redefine the molecular epidemiology of this serovar in Queensland, Australia. Journal of Medical Microbiology 2006; 55: 15491557.
19.Pavan, ME, et al. Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat analysis (MLVA). Comparative Immunology, Microbiology & Infectious Diseases 2011; 34: 135141.
20.Pavan, ME, et al. Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina. Revista Argentina de Microbiología 2011; 43: 251255.
21.Zhang, CC, et al. Application of multiple locus variable- number tandem repeat analysis (MLVA) on molecular typing of Leptospira interrogans. Chinese Journal of Epidcmiology 2010; 31: 249350.
22.Yalin, W, et al. High prevalence of pathogenic Leptospira in wild and domesticated animals in an endemic area of China. Asian Pacific Journal of Tropical Medicine 2011; 4: 841845.
23.de Faria, MT, et al. Carriage of Leptospira interrogans among domestic rats from an urban setting highly endemic for leptospirosis in Brazil. Acta Tropica 2008; 108: 15.
24.Subharat, S, et al. Investigation of localisation of Leptospira spp. in uterine and fetal tissues of non-pregnant and pregnant farmed deer. New Zealand Veterinary Journal 2010; 58: 281285.
25.Ivanova, S, et al. Leptospira and rodents in cambodia: environmental determinants of infection. American Journal of Tropical Medicine and Hygiene 2012; 86: 10321038.
26.Awad-Masalmeh, M, et al. PCR methods to distinguish saprophytic and pathogenic Leptospira strains. Biologia 2002; 57: 17.
27.Gravekamp, C, et al. Detection of seven species of pathogenic leptospires by PCR using two sets of primers. Journal of General Microbiology 1993; 139: 16911700.
28.Guo, SH, Deng, ZH, Li, JH. Analysis of leptospirosis epidemic in 31 provinces (1991–2005). Journal Public Health Preventive Medicine 2006; 6: 810.
29.Yang, MW, Mo, RJ. Exploration of space distribution on Leptospirosis epidemic focus with host animal. Practical Preventive Medicine 2007; 14: 4654.
30.Li, SJ, et al. Molecular Typing of Leptospira interrogans strains isolated from Rattus tanezumi in Guizhou province, Southwest of China. Biomedical and Environmental Sciences 2012; 25: 373380
31.Meerburg, BG, Singleton, GR, Kijlstra, A. Rodent-borne diseases and their risks for public health. Critical Reviews in Microbiology 2009; 35: 221270.
32.Salaün, L, et al. Application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of leptospirosis. Journal of Clinical Microbiology 2006; 44: 39543962.

Keywords

Related content

Powered by UNSILO

Molecular typing of Leptospira spp. strains isolated from field mice confirms a link to human leptospirosis

  • S. J. LI (a1), D. M. WANG (a1), C. C. ZHANG (a2), X. W. LI (a2), H. M. YANG (a2), K. C. TIAN (a1), X. Y. WEI (a1), Y. LIU (a1), G. P. TANG (a1), X. G. JIANG (a2) and J. YAN (a3)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.