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Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

Published online by Cambridge University Press:  01 April 1999

M. AKIBA
Affiliation:
Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki, 305-0856, Japan
T. MASUDA
Affiliation:
Department of Microbiology, Shizuoka Prefectural Institute of Public Health and Environmental Science, Shizuoka, Shizuoka, 420, Japan
T. SAMESHIMA
Affiliation:
Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki, 305-0856, Japan
K. KATSUDA
Affiliation:
Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki, 305-0856, Japan
M. NAKAZAWA
Affiliation:
Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki, 305-0856, Japan
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Abstract

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A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

Type
SHORT PAPER
Copyright
© 1999 Cambridge University Press