Group B streptococci (GBS) were isolated from bovine bulk milk and from vaginas and throats of antenatal and postnatal women using TKT and rapid GBS media. Sixty-three of 529 (12%) bovine bulk milk samples, 9 of 48 (19%) vaginal and 3 of 48 (6%) throat samples were positive. Both bovine and human beta haemolytic isolates were characterized biochemically and serologically. Pigment production was a characteristic of both human and bovine beta haemolytic isolates. The majority (88%) of human isolates fermented salicin and not lactose and most bovine isolates were either lactose positive/salicin positive (41%) or lactose positive/salicin negative (38%). Human and bovine isolates were 100% and 85% typable respectively. Serotype distribution was similar in the bovine and human populations with serotype la, lc and lll being most common in both. Fermentation of sugars showed major differences between bovine and human isolates but similarity in serotype distribution suggests some genetic relationship.