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Iron restriction and the growth of Salmonella enteritidis

Published online by Cambridge University Press:  15 May 2009

H. Chart  and
B. Rowe
H. Chart
Affiliation:
Division of Enteric Pathogens. Central Public Health Laboratory, 61 Colindale Avenue, London, UK
B. Rowe
Affiliation:
Division of Enteric Pathogens. Central Public Health Laboratory, 61 Colindale Avenue, London, UK
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Strains of Salmonella enteritidis were examined for their ability to remove ferricions from the iron chelating agents ovotransferrin, Desferal and EDDA. Growth of S. enteritidis phage type (PT) 4 (SE4) in trypticase soy broth containing ovotransferrin resulted in the expression of iron regulated outer membrane proteins (OMPs) of 74. 78 and 81 kDa. and unexpectedly the repression of expression of OMP C. The 38 MDa ‘mouse virulence’ plasmid was not required for the expression of the iron-regulated OMPs (IROMPs). SE4 was able to obtain iron bound to the iron chelator Desferal and EDDA without expressing a high-affinity iron uptake system. Strains of S. enteritidis belonging to PTs 7. 8, 13a, 23. 24 and 30 were also able to remove ferric ions from Desferal and EDDA without expressing a high-affinity iron uptake system. We conclude that strains of SE4 possess a high-affinity iron sequestering mechanism that can readily remove iron from ovotransferrin. It is likely that iron limitation, and not iron restriction, is responsible for the bacteriostatic properties of fresh egg whites.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 1 , February 1993 , pp. 41 - 47
Copyright
Copyright © Cambridge University Press 1993

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