Skip to main content Accessibility help
×
Home

Introduction and prolonged circulation of G12 rotaviruses in Sicily

  • G. M. GIAMMANCO (a1), F. BONURA (a1), F. DI BERNARDO (a2), A. CASCIO (a3), G. FERRERA (a4), P. DONES (a2), L. SAPORITO (a1), A. COLLURA (a2), D. M. TERRANOVA (a2), M. VALENZISE (a5), M. T. ALLÙ (a6), N. CASUCCIO (a7), M. PALERMO (a8), K. BÁNYAI (a9), V. MARTELLA (a10) and S. DE GRAZIA (a1)...

Summary

Genotype G12 strains are now considered to be the sixth most prevalent human rotaviruses worldwide. In two Sicilian cities, Palermo and Messina, surveillance of rotavirus circulation performed since 1985 and 2009, respectively, did not detect G12 strains until 2012. From 2012 to 2014 rotavirus infection was detected in 29·7% of 1647 stool samples collected from children admitted for acute gastroenteritis to three Sicilian hospitals in Palermo, Messina and Ragusa. In 2012, G12P[8] was first detected in Palermo and then in Messina where it represented the second most frequent genotype (20% prevalence) after G1P[8]. Thereafter, G12 strains continued to circulate in Sicily, showing a marked prevalence in Ragusa (27·8%) in 2013 and in Palermo (21%) and Messina (16·6%) in 2014. All but one of the Sicilian G12 strains carried a P[8] VP4 genotype, whereas the single non-P[8] rotavirus strain was genotyped as G12P[9]. Phylogenetic analysis of the VP7 and VP4 sequences allowed distinction of several genetic lineages and separation of the G12P[8] strains into three cluster combinations. These findings indicate independent introductions of G12 rotavirus strains in Sicily in recent years.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Introduction and prolonged circulation of G12 rotaviruses in Sicily
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Introduction and prolonged circulation of G12 rotaviruses in Sicily
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Introduction and prolonged circulation of G12 rotaviruses in Sicily
      Available formats
      ×

Copyright

Corresponding author

* Author for correspondence: Professor G. M. Giammanco, Department of Health Promotion Sciences and Mother and Child Care, University of Palermo, Via del Vespro 133, I-90127, Italy. (Email: giovanni.giammanco@unipa.it)

References

Hide All
1. Parashar, UD, et al. Global mortality associated with rotavirus disease among children in 2004. Journal of Infectious Diseases 2009; 200 (Suppl. 1): S9S15.
2. Estes, MK, Kapikian, AZ. Rotaviruses, 5th edn. Philadelphia: Lippincott, Williams and Wilkins, 2007, pp. 19171974.
3. Papp, H, et al. Global distribution of group A rotavirus strains in horses: a systematic review. Vaccine 2013; 31: 56275633.
4. Banyai, K, et al. Systematic review of regional and temporal trends in global rotavirus strain diversity in the pre rotavirus vaccine era: insights for understanding the impact of rotavirus vaccination programs. Vaccine 2012; 30 (Suppl. 1): A122130.
5. Cilla, G, et al. Rotavirus genotypes in children in the Basque Country (North of Spain): rapid and intense emergence of the G12[P8] genotype. Epidemiology and Infection 2013; 141: 868874.
6. Das, S, et al. Emergence of novel human group A rotavirus G12 strains in India. Journal of Clinical Microbiology 2003; 41: 27602762.
7. Gomez, MM, et al. Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil. Infection Genetics and Evolution 2014; 28: 385388.
8. Pongsuwanna, Y, et al. Detection of a human rotavirus with G12 and P[9] specificity in Thailand. Journal of Clinical Microbiology 2002; 40: 13901394.
9. Rahman, M, et al. Evolutionary history and global spread of the emerging G12 human rotaviruses. Journal of Virology 2007; 81: 23822390.
10. Samajdar, S, et al. Changing pattern of human group A rotaviruses: emergence of G12 as an important pathogen among children in eastern India. Journal of Clinical Virology 2006; 36: 183188.
11. Seheri, M, et al. Update of rotavirus strains circulating in Africa from 2007 through 2011. Pediatric Infectious Diseases Journal 2014; 33 (Suppl. 1): S7684.
12. Shinozaki, K, et al. Characterization of human rotavirus strains with G12 and P[9] detected in Japan. Journal of Medical Virology 2004; 73: 612616.
13. Iturriza-Gomara, M, et al. Rotavirus genotypes co-circulating in Europe between 2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative strain surveillance network. Epidemiology and Infection 2011; 139: 895909.
14. Mwenda, JM, et al. Burden and epidemiology of rotavirus diarrhea in selected African countries: preliminary results from the African Rotavirus Surveillance Network. Journal of Infectious Diseases 2010; 202 (Suppl.): S5S11.
15. Colomba, C, et al. Viral gastroenteritis in children hospitalised in Sicily, Italy. European Journal of Clinical Microbiology and Infectious Diseases 2006; 25: 570575.
16. De, Grazia S, et al. Data mining from a 27-years rotavirus surveillance in Palermo, Italy. Infection Genetics and Evolution 2014; 28: 377384.
17. Iturriza-Gomara, M, et al. Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses. Journal of Virological Methods 1999; 78: 93103.
18. Arista, S, et al. Identification of human rotavirus strains with the P[14] genotype by PCR. Journal of Clinical Microbiology 1999; 37: 27062708.
19. Banerjee, I, et al. Modification of rotavirus multiplex RT-PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India. Journal of Medical Virology 2007; 79: 14131421.
20. Gouvea, V, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. Journal of Clinical Microbiology 1990; 28: 276282.
21. Gouvea, V, Santos, N, Timenetsky, Mdo C. Identification of bovine and porcine rotavirus G types by PCR. Journal of Clinical Microbiology 1994; 32: 13381340.
22. Iturriza-Gomara, M, Kang, G, Gray, J. Rotavirus genotyping: keeping up with an evolving population of human rotaviruses. Journal of Clinical Virology 2004; 31: 259265.
23. Thompson, JD, Higgins, DG, Gibson, TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 1994; 22: 46734680.
24. Tamura, K, et al. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 2013; 30: 27252729.
25. Taniguchi, K, et al. Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61A) with different serotype specificity from serotype 6. Nucleic Acids Research 1990; 18: 4613.
26. Cunliffe, NA, et al. Serotype G12 rotaviruses, Lilongwe, Malawi. Emerging Infectious Diseases 2009; 15: 8790.
27. Espejo, PW, et al. Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes. Transactions of the Royal Society of Tropical Medicine and Hygiene 2014; 108: 425430.
28. Komoto, S, et al. Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Kenya: identification of porcine-like NSP4 genes. Infection Genetics and Evolution 2014; 27: 277293.
29. Mandile, MG, et al. Surveillance of group A Rotavirus in Buenos Aires 2008–2011, long lasting circulation of G2P[4] strains possibly linked to massive monovalent vaccination in the region. Journal of Clinical Virology 2014; 60: 282289.
30. Ndze, VN, et al. One year survey of human rotavirus strains suggests the emergence of genotype G12 in Cameroon. Journal of Medical Virology 2013; 85: 14851490.
31. Banyai, K, et al. Emergence of serotype G12 rotaviruses, Hungary. Emerging Infectious Diseases 2007; 13: 916919.
32. Kokkinos, PA, et al. Rare types of rotaviruses isolated from children with acute gastroenteritis in Patras, Greece. Intervirology 2013; 56: 237241.
33. Pietsch, C, Liebert, UG. Human infection with G12 rotaviruses, Germany. Emerging Infectious Diseases 2009; 15: 15121515.
34. Steyer, A, et al. Rotavirus genotypes in Slovenia: unexpected detection of G8P[8] and G12P[8] genotypes. Journal of Medical Virology 2007; 79: 626632.
35. Iturriza-Gomara, M, Gray, J, EuroRotanet. 5th Year EuroRotaNet Report; 2012.
36. Delogu, R, et al. Unexpected spreading of G12P[8] rotavirus strains among young children in a small area of central Italy. Journal of Medical Virology 2015; 87: 12921302.
37. Matthijnssens, J, et al. Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Molecular Biology and Evolution 2010; 27: 24312436.
38. Wangchuk, S, et al. Dominance of emerging G9 and G12 genotypes and polymorphism of VP7 and VP4 of rotaviruses from Bhutanese children with severe diarrhea prior to the introduction of vaccine. PLoS ONE 2014; 9: e110795.
39. De, Grazia S, et al. Identification of a multi-reassortant G12P[9] rotavirus with novel VP1, VP2, VP3 and NSP2 genotypes in a child with acute gastroenteritis. Infection Genetics and Evolution 2015; 35: 3437.
40. Ide, T, et al. Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar. PLoS ONE 2015; 10: e0124965.
41. Payne, DC, et al. Effectiveness of pentavalent and monovalent rotavirus vaccines in concurrent use among US children <5 years of age, 2009–2011. Clinical Infectious Diseases 2013; 57: 1320.
42. Steele, AD, et al. Human rotavirus vaccine Rotarix provides protection against diverse circulating rotavirus strains in African infants: a randomized controlled trial. BMC Infectious Diseases 2012; 12: 213.
43. Linhares, AC, et al. Rotavirus serotype G9 is associated with more-severe disease in Latin America. Clinical Infectious Diseases 2006; 43: 312314.
44. de Rougemont, A, et al. Molecular and clinical characterization of rotavirus from diarrheal infants admitted to pediatric emergency units in France. Pediatric Infectious Diseases Journal 2011; 30: 118124.

Keywords

Introduction and prolonged circulation of G12 rotaviruses in Sicily

  • G. M. GIAMMANCO (a1), F. BONURA (a1), F. DI BERNARDO (a2), A. CASCIO (a3), G. FERRERA (a4), P. DONES (a2), L. SAPORITO (a1), A. COLLURA (a2), D. M. TERRANOVA (a2), M. VALENZISE (a5), M. T. ALLÙ (a6), N. CASUCCIO (a7), M. PALERMO (a8), K. BÁNYAI (a9), V. MARTELLA (a10) and S. DE GRAZIA (a1)...

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed