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Incubation at 44°C. as a test for faecal coli

  • L. F. L. Clegg (a1), H. P. Sherwood (a1) and R. W. Dodgson (a2)

Extract

1. The use of Esch. coli alone as an index of faecal pollution for shellfish, and the correlation between the 44° C. MacConkey test and citrate tests are discussed.

2. The mercury-toluene thermo-regulator used in these experiments, which gives a maximum variation of ± 0.1° C., is discussed briefly and illustrated.

3. Experiments are described in which 522 colonies from polluted shellfish were isolated, inoculated into MacConkey's broth and incubated at temperatures of 37° C. and at successive 1° intervals from 41 to 46° C., in accurately controlled water-baths. An almost perfect negative correlation was found to exist between 44° C. incubation and the citrate test.

4. It appeared that temperatures above 44° C. are detrimental to the growth of Esch. coli.

5. Certain cultures of citrate-negative lactose fermenters at 37° C., which were inhibited at 44° C., were found on further investigation to be mostly intermediate types.

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References

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Incubation at 44°C. as a test for faecal coli

  • L. F. L. Clegg (a1), H. P. Sherwood (a1) and R. W. Dodgson (a2)

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