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Incidence and nature of peritoneal catheter biofilm determined by electron and confocal laser scanning microscopy

Published online by Cambridge University Press:  15 May 2009

S. P. Gorman ,
C. G. Adair  and
W. M. Mawhinney
S. P. Gorman
Affiliation:
School of Pharmacy, Medical Biology Centre, The Queen's University of Belfast, Belfast BT9 7BL, UK
C. G. Adair
Affiliation:
School of Pharmacy, Medical Biology Centre, The Queen's University of Belfast, Belfast BT9 7BL, UK
W. M. Mawhinney
Affiliation:
School of Pharmacy, Medical Biology Centre, The Queen's University of Belfast, Belfast BT9 7BL, UK
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Summary

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Thirty-two Tenckhoff catheters retrieved from continuous ambulatory peritoneal dialysis patients with a history of peritonitis were examined for microbial biofilm. Confocal laser scanning microscopy was successfully employed to visualize bacteria in biofilm occluded from view by scanning electron microscopy. Occluded but viable microbial biofilm was associated with 17 (81%) catheters from patients free from infection following renal transplant. Mixed isolate biofilm with two or more isolates of coagulase-negative staphylococci or Staphylococcus aureus was found on 41% of these catheters. Clearly visible viable biofilm consisting exclusively of Pseudomonas aeruginosa occurred on all four catheters removed due to recurrent peritonitis. Five (71%) catheters retrieved from patients transferred to haemodialysis had viable biofilm. Antibiotic sensitivities of the biofilm isolates were similar in profile to those reported for non-biofilm isolates from infected dialysate. Persistence of catheter biofilm despite direct contact with therapeutic levels of antibiotics in peritoneal dialysate requires that attention be directed towards improving antibiotic efficacy against peritonitis-causing bacteria in biofilm form.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 3 , June 1994 , pp. 551 - 559
Copyright
Copyright © Cambridge University Press 1994

References

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