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The immunogenicity of heat-inactivated vaccinia virus in rabbits*

Published online by Cambridge University Press:  15 May 2009

C. R. Madeley
C. R. Madeley
Affiliation:
Department of Bacteriology, University of Liverpool
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Rabbits were immunized by multiple intradermal injections followed by six intravenous doses of vaccinia virus inactivated by heating to 65° C. Particular attention was paid to confirming that the virus used was fully inactivated and incapable of reactivation. The immunized rabbits developed neutralizing and other antibodies to a titre comparable with those developed in response to live virus, but multiple intravenous injections were required to elicit a maximum titre. A qualitatively different response was seen only in immunodiffusion tests in agar gel where two or three fewer lines developed with antisera to heated virus than with those to live virus. The rabbits were subsequently challenged intradermally either with vaccinia and cowpox or with rabbitpox. They showed some immunity to vaccinia and cowpox, compared with normal controls, but less than that elicited by live virus. Their resistance to lethal doses of rabbitpox was life-saving though some rabbits did develop lesions. Later rechallenge of the rabbits showed that they had not developed further immunity, in distinction from the normal controls. The implications of these findings are discussed.

I wish to thank the National Fund for Research into Poliomyelitis and Other Crippling Diseases for their generous support in providing a Research Fellowship during which this work was done. I wish to thank also Professor A. W. Downie F.R.S. and the Staff of the Department of Bacteriology, University of Liverpool, for their encouragement and assistance respectively.

Type
Research Article
Information
Journal of Hygiene , Volume 66 , Issue 1 , March 1968 , pp. 89 - 107
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Amies, C. R. (1961). Loss of immunogenic properties of vaccinia virus inactivated by formaldehyde. Can. J. Microbiol. 7, 141.Google Scholar
Appleyard, G. (1961). An immunizing antigen from rabbitpox and vaccinia viruses. Nature, Lond. 190, 465.Google Scholar
Bedson, H. S. & Duckworth, M. J. (1963). Rabbitpox: An experimental study of the pathways of infection in rabbits. J. Path. Bact. 85, 1.Google Scholar
Chu, C. M. (1948 a). Studies on vaccinia haemagglutinin. I. Some physico-chemical properties. J. Hyg., Camb. 46, 42.Google Scholar
Chu, C. M. (1948 b). Studies on vaccinia haemagglutinin. II. Some immunological properties. J. Hyg., Camb. 46, 49.Google Scholar
Collier, L. H., Mcclean, D. & Vallet, L. (1955). The antigenicity of ultra-violet irradiated vaccinia virus. J. Hyg., Camb. 53, 513.Google ScholarPubMed
Craigie, J. (1932). The nature of the vaccinia flocculation reaction and observations on the elementary bodies of vaccinia. Br. J. exp. Path. 13, 259.Google Scholar
Dales, S. & Kajioka, R. (1964). The cycle of multiplication of vaccinia virus in Earle's strain ‘L’ cells. I. Uptake and penetration. Virology 24, 278.Google Scholar
Downie, A. W. (1965). In Viral and Rickettsial Infections of Man, 4th ed., p. 936, edited by Horsfall, F. L. and Tamm, I.. Philadelphia, J. B. Lippincott, Co.Google Scholar
Downie, A. W. & Macdonald, A. (1950). A study of the pox viruses by complement fixation and inhibition of complement fixation methods. J. Path. Bact. 62, 389.Google Scholar
Dulbecoo, R. & Vogt, M. (1954). Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. Med. 99, 167.Google Scholar
Fenner, F. & Burnet, F. M. (1957). A short description of the poxvirus group (vaccinia and related viruses). Virology 4, 305.Google Scholar
Fulgintti, V. A., Arthur, J., Perlman, D. S. & Kempe, C. H. (1966). Serious local reactions following live measles virus immunization in previous killed vaccine recipients. J. Pediat. 69, 891.CrossRefGoogle Scholar
Galasso, G. J. & Sharp, D. G. (1963). Homologous inhibition with heated and ultraviolet-treated vaccinia virus in cultures of L cells. Virology 20, 1.CrossRefGoogle ScholarPubMed
Gordon, M. H. (1925). Studies on the viruses of vaccinia and variola. Section II Part 9, p. 41. Experimental studies of active immunity to vaccinia in the rabbit. Spec. Sep. Ser. med. Res. Coun. no. 98.Google Scholar
Herrlich, A. (1959). Uber Vakzine-Antigen. Versuch einer Prophylaxe neuraler Impf-schãden. Münch, med. Wschr. 101, 12.Google Scholar
Herrlich, A. (1964). Welchen Nutzen hat die Prophylaxe der Postvakzinalen Enzephalitis? Dt. med. Wschr. 89, 968.CrossRefGoogle Scholar
Hoagland, C. L., Smadel, J. E. & Rivers, T. M. (1940). Constituents of elementary bodies of vaccinia. I. Certain basic analyses and observations on lipid components of the virus. J. exp. Med. 71, 737.CrossRefGoogle ScholarPubMed
Jansen, J. (1941). Tödliche Infektionen von Kaninchen durch ein filtrierbares virus. Zentbl. Bakt. ParasitKde. Abt. I. Orig. 148, 65.Google Scholar
Kaplan, C. (1962). A non-infectious smallpox vaccine. Lancet ii, 1027.CrossRefGoogle Scholar
Kaplan, C., Benson, P. F. & Butler, N. R. (1965). Immunogenicity of ultra-violet-irradiated, non-infectious vaccinia-virus vaccine in infants and young children. Lancet i, 573.Google Scholar
Kaplan, C., Mcclean, D. & Vallet, L. (1962). A note on the immunogenicity of ultra-violet irradiated vaccinia virus in man. J. Hyg., Camb. 60, 79.CrossRefGoogle ScholarPubMed
Kligler, I. J. & Bernkopf, H. (1935). Immunization of rabbits with inactive vaccinia virus. Proc. Soc. exp Biol. Med. 33, 226.Google Scholar
Mccarthy, K. & Downie, A. W. (1948). An investigation of immunological relationships between the viruses of variola, vaccinia, cowpox and ectromelia by neutralization tests on the chorio-allantois of chick embryos. Br. J. exp. Path. 29, 501.Google Scholar
Mccarthy, K. & Helbert, D. (1960). A comparison of the haemagglutinins of variola, alastrim, vaccinia, cowpox and ectromelia viruses. J. Path. Bact. 79, 416.Google Scholar
Mcneill, T. A. (1965). The antibody response of rabbits to inactivated vaccinia virus. J. Hyg., Camb. 63, 525.Google Scholar
Mcneill, T. A. (1966). The development of skin resistance and hypersensitivity following inactivated vaccinia virus vaccines in rabbits. J. Hyg., Camb. 64, 23.Google Scholar
Mahnel, H. (1961). Tierexperimentelle Untersuchungen mit Vakzine-Antigen. Der Einfluss einer Vorimpfung mit inaktiviertem Antigen auf die nachfolgende Vakzininfektion des Kaninchens. Arch. ges. Virusforsch. 10, 529.CrossRefGoogle Scholar
Nakagawa, S. (1924). Ueber das Koktoimmunogen des Variola-Vakzine Virus. Z. Immun-Forsch. exp. Ther. 39, 563.Google Scholar
Parker, R. F. & Rivers, T. M. (1936). Immunological and chemical investigations of vaccine virus. III. Response of rabbits to inactive elementary bodies of vaccinia and to virus-free extracts of vaccine virus. J. exp. Med. 63, 69.CrossRefGoogle ScholarPubMed
Rauh, L. W. & Schmidt, R. (1965). Measles immunization with killed virus vaccine. Am. J. Dis. Child. 109, 232.CrossRefGoogle ScholarPubMed
Ramanarao, A. V. (1962). The immunogenicity of inactivated vaccinia virus. J. Path. Bact. 84, 367.Google Scholar
Rondle, C. J. M. & Dumbell, K. R. (1962). Antigens of cowpox virus. J. Hyg., Camb. 60, 41.Google Scholar
Woodroofe, G. M. (1960). The heat inactivation of vaccinia virus. Virology 10, 379.CrossRefGoogle ScholarPubMed