Skip to main content Accessibility help
×
Home

Guanidine and heat sensitivity of foot-and-mouth disease virus (FMDV) strains

  • P. F. Nettleton (a1), Marilyn J. Davies (a1) and M. M. Rweyemamu (a1)

Summary

A study of the ability of 49 strains of FMD virus to replicate in BHK-21 monolayer cells maintained under a standard agar overlay containing 5·2 mM guanidine hydrochloride and to withstand heat inactivation at 54°C for 1 h showed that strains belonging to serotypes C, O and Asia 1 were generally more resistant to guanidine and heat stable than the SAT 1, 2 and 3 serotypes. The type A viruses as a whole occupied an intermediate position between these two groups.

In vitro passage in BHK-21 cells influenced the guanidine sensitivity of 3(O, C and SAT 3) of the 7 FMD serotypes suggesting that this is not a stable genetic marker. Heat stability of the FMD viruses, however, did not change on passage, suggesting that this is a stable characteristic inherent in any homogeneous FMD virus population.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Guanidine and heat sensitivity of foot-and-mouth disease virus (FMDV) strains
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Guanidine and heat sensitivity of foot-and-mouth disease virus (FMDV) strains
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Guanidine and heat sensitivity of foot-and-mouth disease virus (FMDV) strains
      Available formats
      ×

Copyright

References

Hide All
Bachrach, H. (1964). Foot and mouth disease virus: Structure and mechanism of degradation as deduced from absorbance-temperature relationships. Journal of Molecular Biology 8, 348358.
Bachrach, H. L., Patty, R. E. & Pledger, R. A. (1960). Thermal resistant populations of foot-and-mouth disease virus. Proceedings of the Society for Experimental Biology and Medicine 103, 540542.
Black, D. N. & Brown, F. (1969). Effect of Actinomycin D and Guanidine on the formation of a ribonucleic acid polymerase induced by foot-and-mouth disease virus and on the replication of virus and viral ribonucleic acid. Biochemical Journal 112, 317323.
Brown, F. (1976). Replication of Picornaviruses. In Chemotherapy, vol. 6. (ed. Williams, J. D. and Geddes, A. M.). Plenum Publishing Corporation.
Brown, F. & Crick, J. (1958). Application of agar gel precipitin tests to the study of the virus of foot-and-mouth disease. Virology 5 (1), 133144.
Brown, F., Martin, S. J. & Underwood, B. (1966). A study of the kinetics of protein and RNA synthesis induced by foot-and-mouth disease virus. Biochemica et Biophysica Acta 129, 166177.
Brown, F. & Wild, F. (1966). The effect of heat on the structure of foot and mouth disease virus and the viral ribonucleic acid. Biochemica et Biophysica Acta 119, 301308.
Capstick, P. B., Telling, R. C., Chapman, W. G. & Stewart, D. L. (1962). Growth of a cloned strain of hamster kidney cells in suspended cultures and their susceptibility to the virus of foot-and-mouth disease. Nature, Lond. 195, 11631164.
Chema, S. & Rweyemamu, M. M. (1978). Selection of SAT 2 FMDV strains for East Africa. Bulletin de l'Office International des Epizooties 89, 887898.
Cowan, K. M. (1973). Antibody responses to viral antigen. Advances in Immunology 17, 195253.
Doel, T. R. & Baccarini, P. J. (1981). Thermal stability of foot and mouth disease virus. Archives of Virology 70, 2132.
Lake, J. R., Priston, R. A. J. & Slade, W. R. (1975). A genetic recombination map of foot-and-mouth disease virus. Journal of General Virology 27, 355367.
McCahon, D., Slade, W. R., Priston, R. A. J. & Lake, J. R. (1977). An extended genetic recombination map for foot-and-mouth disease virus. Journal of General Virology 35, 555565.
Mackenzie, J. S. & Slade, W. R. (1975). Evidence for recombination between two different immunological types of foot-and-mouth disease virus. Australian Journal of Experimental Biology and Medical Science 53 (4), 251256.
Mowat, G. N. (1974). Potency of BHK-produced foot-and-mouth disease vaccines after storage. Bulletin de l'Office International des Epizooties 81, 11511154.
Mowat, G. N. & Chapman, W. G. (1962). Growth of foot-and-mouth disease virus in a fibroblastic cell line derived from hamster kidneys. Nature, Land. 194, 253255.
Nick, H. & Ahl, R. (1976). Inhibitors of foot-and-mouth disease virus. II. Temperature dependence of the effect of guanidine on virus growth. Archives of Virology 52, (1–2), 7183.
Pay, T. W. F., Rweyamamu, M. M. & O'Reilly, K. J. (1978). Experiences with type SAT 2 foot-and-mouth disease vaccines in Southern Africa. Bulletin de l'Office International des Epizooties 89, 899927.
Pay, T. W. F. & Schermbrucker, C. G. (1974). Production of foot-and-mouth disease vaccines against various types and subtypes. Bulletin de l'Office International des Epizooties 81, 12611270.
Pereira, H. G. (1977). Subtyping of foot-and-mouth disease virus. Developments in Biological Standardisation 35, 167174.
Pringle, C. R. (1964 a). Inhibition of multiplication of foot-and-mouth disease virus by guanidine hydrochloride. Nature, Lond. 204, 10121013.
Pringle, C. R. (1964 b). Genetic aspects of the thermal inactivation of foot-and-mouth disease virus strains. Bulletin de l'Office International des Epizooties 61, 619628.
Pringle, C. R. (1965). Evidence of genetic recombination in foot-and-mouth disease virus. Virology 25, 4855.
Pringle, C. R. (1969). Electrophoretic properties of foot-and-mouth disease virus strains and the selection of intra-strains mutants. Journal of General Virology 4, 541555.
Robson, K. J. H., Harris, T. J. R. & Brown, F. (1977). An assessment by competition hybridisation of the sequence homology between the RNA's of the seven serotypes of foot-and-mouth disease virus. Journal of General Virology 37, 271276.
Rweyemamu, M. M., Booth, J. C., Head, M. & Pay, T. W. F. (1978). Microneutralisation tests for serological typing and subtyping of foot-and-mouth disease virus strains. Journal of Hygiene 81, 107123.
Rweyemamu, M. M., Terry, G. & Pay, T. W. F. (1979). Stability and immunogenicity of empty particles of foot-and-mouth disease virus. Archives of Virology 59, 6979.

Guanidine and heat sensitivity of foot-and-mouth disease virus (FMDV) strains

  • P. F. Nettleton (a1), Marilyn J. Davies (a1) and M. M. Rweyemamu (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed