Hostname: page-component-848d4c4894-sjtt6 Total loading time: 0 Render date: 2024-07-07T00:42:57.385Z Has data issue: false hasContentIssue false

The growth of Leptospira australis B in the kidneys of mice in the incipient experimental carrier state

Published online by Cambridge University Press:  15 May 2009

S. Faine
Affiliation:
Department of Bacteriology, University of Sydney
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The growth of L. australis B in the kidneys of young mice which become carriers was followed after experimental intraperitoneal infection. There was a primary growth corresponding to generalized acute infection and terminated at the time of appearance of antibody. A secondary growth bf leptospirae followed in the kidneys alone about 7–10 days after infection, coinciding with the onset of leptospiruria and recovery from infection. Subsequently mice carried about 106–107 leptospirae in their kidneys permanently.

The leptospirae in the urine or in the kidneys of carriers were resistant to the action of antibody in the serum or urine of the host animal, or in rabbit antisera prepared against mouse-renal leptospirae or against cultured leptospirae of the infecting strain. No antigenic differences were detected between renal and cultured leptospirae. An analogous situation is the growth in vitro of leptospirae in homologous antiserum. The mechanism permitting growth of leptospirae in homologous antiserum in vivo or in vitro is unknown.

The carrier condition results from the ability of virulent leptospirae to (i) grow in the host and produce lesions in the primary, acute generalized infection, (ii) grow in renal tubules in the presence of antibody.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1962

References

Babudieri, B. (1958). Animal reservoirs of leptospires. Ann. N.Y. Acad. Sci. 70, 393.CrossRefGoogle ScholarPubMed
Broom, J. C. (1944). Footnote to abstract. Bull. Hyg. 19, 396.Google Scholar
Dinger, J. E. & Verschaffelt, F. (1930). Recherches expérimentales sur quelques souches de leptospires. Ann. Inst. Pasteur, 45, 396.Google Scholar
Fains, S. (1957 a). Virulence in leptospira. I. Reactions of guinea-pigs to experimental infection with Leptospira icterohaemorrhagiae. Brit. J. exp. Path. 38, 1.Google Scholar
Faine, S. (1957 b). Virulence in leptospira. II. The growth in vivo of virulent Leptospira icterohaemorrhagiae. Brit. J. exp. Path. 38, 8.Google ScholarPubMed
Faine, S. (1962 a). Factors affecting the development of the carrier state in leptospirosis. J. Hyg., Camb., 60, 427.Google ScholarPubMed
Faine, S. (1962 b). Antibody in renal tubules of mice. Aust. J. exp. Biol. (In the Press.)Google Scholar
Ido, Y., Hoki, R., Ito, H. & Wani, H. (1916). The prophylaxis of Weil's Disease. J. exp. Med. 24, 471.CrossRefGoogle ScholarPubMed
Kwee, Tat Tjong (1940). Over de positie der leptospiren in de flier bij chronische uitscheiders. Thesis, Batavia. Quoted by Van Thiel (1948), P. 51.Google Scholar
Larson, A. D., Treick, R. W., Edwards, C. L. & Cox, C. D. (1959). Growth studies and plate counting of leptospires. J. Bact. 77, 361.CrossRefGoogle ScholarPubMed
Ouchterlony, O. (1958). Diffusion-in-gel methods for immunological analysis. Progr. Allergy, 5, 1.Google ScholarPubMed
Ovary, Z. (1958). Immediate reaction in the skin of experimental animals provoked by antibody—antigen interactions. Progr. Allergy, 5, 459.Google Scholar
Pike, R. M. & Schulze, M. L. (1958). Serologic variants of leptospira types resulting from growth in immune serum. J. Immunol. 81, 172.CrossRefGoogle ScholarPubMed
Proehoeman, S. (1930). Studies over do epidemiologie van de ziekte van Weil, over haren verwekker en de daaraan verwante organismen. Thesis, Amsterdam. Quoted by Van Thiel (1948), p. 117.Google Scholar
Remington, J. S. & Finland, M. (1961). Precipitating antibody in normal human urine. Proc. Soc. exp. Biol., N.Y., 107, 765.CrossRefGoogle ScholarPubMed
Schüffner, W. & Bohlander, H. (1942). Klinische und bakteriologische Beobachtung einer Laboratoriumsinfektion mit Schlammfieber. Anhaltende Leptospirurie. Zbl. Bakt. (1 Abt. Orig.), 149, 193.Google Scholar
Schüffner, W. & Bohlander, H. (1943). Ueber den verschiedenen Verlauf der durch Leptospiren hervorgerufenen Nierenprozesses bei Feldmaus und Ratte. Z. ImmunForsch. 104, 237.Google Scholar
Schüffner, W. & Mochtar, A. (1927). Versuche zur Aufteilung von Leptospirenstammen mit einleitenden Bemerkungen uber den Verlauf von Agglutination und Lysis. Zbl. Bakt. (1 Abt. Orig.), 101, 405.Google Scholar
Stuart, R. D. (1956). The importance of urinary antibodies in the diagnosis of leptospirosis. Canad. J. Microbiol. 2, 288.CrossRefGoogle ScholarPubMed
Van Der Hoeden, J. (1936). Anticorps spécifiques de la maladie de Well dans l'urine. Ann. Inst. Pasteur, 56, 206.Google Scholar
Thiel, Van P. H. (1948). The Leptospiroses. Leiden: Universitaire Pers.Google Scholar