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Genetic relationships among Neisseria gonorrhoeae serovars analysed by multilocus enzyme electrophoresis

Published online by Cambridge University Press:  15 May 2009

C. L. Poh ,
J. C. Ocampo  and
G. K. Loh
C. L. Poh
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 0511
J. C. Ocampo
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 0511
G. K. Loh
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 0511
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Abstract

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Multilocus enzyme electrophoretic analysis was employed to assess the genetic relatedness of Neisseria gonorrhoeae. Based on the diversity of electromorphs at 9 enzyme loci, 16 electrophoretic types (ETs) were estabilished amongst the 65 isolates. The average number of alleles per enzyme locus was 1·7 and the mean genetic diversity per locus was 0·212. The majority of isolates belonged to either ET1 (32·3%) or ET2 (16·9%). No specific correlation of ETs was seen with serovars as the major types, ETs 1 and 2, were found distributed amongst the various serovars. Major serovars such as Bacjk (IB-1/2) and Bajk (IB-3/6) were each represented by 6 or 8 ETs respectively. Analysis of the genetic relationships of ETs to each other showed some clustering of subgroups that were more closely related than others.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 1 , February 1992 , pp. 31 - 38
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Sandstrom, EG, Chen, KCS, Buchanan, TM. Serology of Neisseria gonorrhoeae: coagglutination serogroups WI and WII/III correspond to different outer membrane protein I molecules. Infect Immun 1982; 38: 462–70.CrossRefGoogle ScholarPubMed
2.Tam, IMR, Buchanan, TM, Sandstrom, EG, Holmes, KK, Knapp, JS, Siadak, AW, Nowinski, RC. Serological classification of Neisseria gonorrhoeae with monoclonal antibodies. Infect Immun 1983; 36: 1042–53.CrossRefGoogle Scholar
3.Bygdeman, S, Danielsson, D, Sandstrom, EG. Gonococcal W serogroups in Scandinavia. A study with polyclonal and monoclonal antibodies. Acta Pathol Microbiol Scand Sect B 1983; 91: 293–305.Google ScholarPubMed
4.Knapp, J, Tam, MR, Nowinski, RC, Holmes, KK, Sandstrom, EG. Serological classification of Neisseria gonorrhoeae with use of monoclonal antibodies to gonococcal outer membrane protein I. J Infect Dis 1984; 150: 44–8.CrossRefGoogle ScholarPubMed
5.Selander, DK, Caugant, DA, Ochman, H, Musser, JM, Gilmour, MN, Whittam, TS. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 1986; 51: 873–4.CrossRefGoogle ScholarPubMed
6.Davis, BJ. Disc electrophoresis. Method and application to human serum proteins. Ann NY Acad Sci 1964; 121: 404–27.CrossRefGoogle ScholarPubMed
7.Nei, M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 1978; 89: 583–90.CrossRefGoogle ScholarPubMed
8.Rohlf, FJ. NTSYS–pc. In: Numerical toxonomy and multivariate analysis system user manual, version 1.40. New York: Exeter Publishing Ltd, 1988: 55–64.Google Scholar
9.Selander, RK, Korhonen, TK, Vaisanen-Rhen, V, Williams, PH, Pattison, PE, Caugant, DA. Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infect Immun 1986; 52: 213–22.CrossRefGoogle Scholar
10.Caugant, DA, Mocca, LF, Frasch, CE, Oddvar, Froholm L, Zollinger, WD, Selander, RK. Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern. J Bact 1987; 169: 2781–92.CrossRefGoogle ScholarPubMed
11.Poh, CL, Ocampo, JC, Sng, EH, Bygdeman, SM. Characterization of PPNG and non–PPNG Neisseria gonorrhoeae isolates from Singapore. Genitourin Med. In press.Google Scholar