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First phylogenetic analysis of dengue virus serotype 4 circulating in Espírito Santo state, Brazil, in 2013 and 2014

  • C. R. VICENTE (a1) (a2), C. S. PANNUTI (a3), P. R. URBANO (a3), A. C. FELIX (a3), C. CERUTTI JUNIOR (a2), K.-H. HERBINGER (a4), G. FRÖSCHL (a1) (a4) and C. M. ROMANO (a3)...

Summary

The purpose of the present study was to reconstruct the phylogeny of dengue virus serotype 4 (DENV-4) that was circulating in Espírito Santo state, Brazil, in 2013 and 2014, and to discuss the epidemiological implications associated with this evolutionary hypothesis. Partial envelope gene of eight DENV-4 samples from Espírito Santo state were sequenced and aligned with 72 worldwide DENV-4 reference sequences from GenBank. A phylogenetic tree was reconstructed through Bayesian Inference and the Time of the Most Recent Common Ancestor was estimated. The study detected the circulation of DENV-4 genotype II in Espírito Santo state, which was closely related to strains from the states of Mato Grosso collected in 2012 and of São Paulo sampled in 2015. This cluster emerged around 2011, approximately 4 years after the entry of the genotype II in Brazil through its northern states, possibly imported from Venezuela and Colombia. This is so far the first phylogenetic study of the DENV-4 circulating in Espírito Santo state and shows the importance of an internal route of dengue viral circulation in Brazil to the introduction of the virus into this state.

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Copyright

Corresponding author

*Author for correspondence: C. R. Vicente, Centre for International Health, Medical Centre of the University of Munich and Department of Social Medicine, Federal University of Espírito Santo, Avenida Marechal Campos 1468, 29040-090, Vitória, Brazil. (Email: vicentecrachel@gmail.com)

References

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1. Bhatt, S, et al. The global distribution and burden of dengue. Nature 2013; 496: 504507.
2. Pinho, ACO, et al. Asian genotypes of dengue virus 4 in Brazil. Epidemiology and Infection 2015; 143: 31143117.
3. Rico-Hesse, R. Dengue virus virulence and transmission determinants. Current Topics in Microbiology and Immunology 2010; 338: 4555.
4. Kuhn, RJ, et al. Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell 2002; 108: 717725.
5. Twiddy, SS, Holmes, EC, Rambaut, A. Inferring the rate and time-scale of dengue virus evolution. Molecular Biology and Evolution 2003; 20: 122129.
6. Chen, SP, et al. Identification of a recombinant dengue virus type 1 with 3 recombination regions in natural populations in Guangdong province, China. Archives of Virology 2008; 153: 11751179.
7. Mendez, JA, et al. Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia. Virology Journal 2010; 7: 226.
8. Rodriguez-Barraquer, I, et al. From re-emergence to hyperendemicity: the natural history of the dengue epidemic in Brazil. PLoS Neglected Tropical Diseases 2011; 5: e935.
9. Temporão, JG, et al. Dengue virus serotype 4, Roraima state, Brazil. Emerging Infectious Diseases 2011; 17: 938939.
10. Health Department of Espírito Santo state. Boletim epidemiológico – n°. 1 – 2016 – dengue, chikungunya e zika – semana 13–2016. (http://mosquito.saude.es.gov.br/Media/dengue/Boletim%20Epidemiologico/Boletim%20Epid%20Dengue_01_2016_Final.pdf). Accessed 15 July 2017.
11. Brazilian Ministry of Health. Procedimentos hospitalares do SUS por local de internação – Espírito Santo (http://tabnet.datasus.gov.br/). Accessed 15 July 2017.
12. Brazilian Ministry of Health 2015. Casos de dengue – Brasil, grandes regiões e unidades federadas, 1990 a 2014. (http://portalsaude.saude.gov.br/images/pdf/2015/julho/29/Dengue-at---2014.pdf). Accessed 15 July 2017.
13. Koo, C, et al. Evolution and heterogeneity of multiple serotypes of dengue virus in Pakistan, 2006–2011. Virology Journal 2013; 10: 275.
14. Felix, AC, et al. Low sensitivity of NS1 protein tests evidenced during a dengue type 2 virus outbreak in Santos, Brazil, in 2010. Clinical and Vaccine Immunology 2012; 19: 19721976.
15. Thompson, J, et al. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 1997; 25: 48764882.
16. Posada, D. Jmodeltest: phylogenetic model averaging. Molecular Biology and Evolution 2008; 25: 12531256.
17. Drummond, AJ, Rambaut, A. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 2007; 7: 214.
18. Lanciotti, RS, Gubler, DJ, Trent, DW. Molecular evolution and phylogeny of dengue-4 viruses. Journal of General Virology 1997; 78: 22792286.
19. Nunes, MRT, et al. Phylogeography of dengue virus serotype 4, Brazil, 2010–2011. Emerging Infectious Diseases 2012; 18: 18581864.
20. Foster, JE, et al. Molecular evolution and phylogeny of dengue type 4 virus in the Caribbean. Virology 2003; 306: 126134.
21. Allicock, OM, et al. Phylogeography and population dynamics of dengue viruses in the Americas. Molecular Biology and Evolution 2012; 29: 15331543.
22. Romano, CM, et al. Characterization of dengue virus type 2: new insights on the 2010 Brazilian epidemic. PLoS ONE 2010; 5: e11811.
23. Souza, RP, et al. Dengue virus type 4 phylogenetics in Brazil 2011: looking beyond the veil. PLoS Neglected Tropical Diseases 2011; 5: e1439.
24. Dettogni, RS, Louro, ID. Phylogenetic characterization of dengue virus type 2 in Espírito Santo, Brazil. Molecular Biology Reports 2012; 39: 7180.

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