Skip to main content Accessibility help
×
Home

Feasibility of collecting oral fluid samples in the home setting to determine seroprevalence of infections in a large-scale cohort of preschool-aged children

  • S. E. BARTINGTON (a1), C. PECKHAM (a1), D. BROWN (a2), H. JOSHI (a3) and C. DEZATEUX (a1)...

Summary

Oral fluid is a non-invasive biological sample, which can be returned by post, making it suitable for large-scale epidemiological studies in children. We report our experience of oral fluid collection from 14 373 preschool-aged children in the UK Millennium Cohort Study. Samples were collected by mothers in the home setting following the guidance of trained interviewers, and posted to the laboratory. Samples were received from 11 698 children (81·4%). Children whose mothers were of Black Caribbean ethnicity and who lived in non-English-speaking households were less likely to provide a sample, and those with a maternal history of asthma more likely to provide a sample [adjusted risk ratio (95% CI) 0·85 (0·73–0·98), 0·87 (0·77–0·98) and 1·03 (1·00–1·05) respectively]. Collection of oral fluid samples is feasible and acceptable in large-scale child cohort studies. Formal interpreter support may be required to increase participation rates in surveys that collect biological samples from ethnic minorities.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Feasibility of collecting oral fluid samples in the home setting to determine seroprevalence of infections in a large-scale cohort of preschool-aged children
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Feasibility of collecting oral fluid samples in the home setting to determine seroprevalence of infections in a large-scale cohort of preschool-aged children
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Feasibility of collecting oral fluid samples in the home setting to determine seroprevalence of infections in a large-scale cohort of preschool-aged children
      Available formats
      ×

Copyright

Corresponding author

*Author for correspondence: Miss S. E. Bartington, MRC Centre of Epidemiology for Child Health, UCL Institute of Child Health, 30 Guilford Street, London, UK. (Email: s.bartington@ich.ucl.ac.uk)

References

Hide All
1.George, JR, Fitchen, JH. Future applications of oral fluid specimen technology. American Journal of Medicine 1997; 102: 2125.
2.Nokes, DJ, et al. Has oral fluid the potential to replace serum for the evaluation of population immunity levels? A study of measles, rubella and hepatitis B in rural Ethiopia. Bulletin of the World Health Organisation 2001; 79: 588595.
3.McKie, A, Vyse, A, Maple, C. Novel methods for the detection of microbial antibodies in oral fluid. Lancet Infectious Diseases 2002; 2: 1824.
4.Morris, MC, et al. Oral fluid collection by post – a pilot study of two approaches. Public Health 2002; 116: 113119.
5.Morris, MC, et al. Stability of total and rubella-specific IgG in oral fluid samples: the effect of time and temperature. Journal of Immunological Methods 2002; 266: 111116.
6.von Mutius, E. Allergies, infections and the hygiene hypothesis – the epidemiological evidence. Immunobiology 2007; 212: 433439.
7.Smith, K, Joshi, H. The Millennium Cohort Study. Population Trends 2002; 107: 3034.
8.Plewis, I. Millennium Cohort Study: Technical Report on Sampling, 4th edn. London: Institute of Education, University of London, 2007 (http://www.cls.ioe.ac.uk/studies.asp?section=00010002000100050004). Accessed 24 July 2007.
9.Plewis, I, Ketende, S. Millennium Cohort Study: Technical Report on Response, 1st edn.London: Institute of Education, University of London, 2006. (http://www.cls.ioe.ac.uk/studies.asp?section=00010002000100040006). Accessed 24 July 2007.
10.UK Data Archive. Millennium Cohort Study First Survey (http://www.dataarchive.ac.uk/findingData/snDescription.asp?sn=4683). Accessed 24 July 2007.
11.UK Data Archive. Millennium Cohort Study Second Survey (http://www.dataarchive.ac.uk/findingData/snDescription.asp?sn=5350). Accessed 24 July 2007.
12.Hansen, K. Millennium Cohort Study First and Second Surveys: A Guide to the Datasets, 1st edn.London: Institute of Education, University of London, 2006.
13.GfK NOP Social Research. Millennium Cohort Study – Sweep 2. Technical Report on Fieldwork (http://www.cls.ioe.ac.uk/studies.asp?section=00010002000100040006). Accessed 24 July 2007.
14.Vyse, AJ, Cohen, BJ, Ramsay, ME. A comparison of oral fluid collection devices for use in the surveillance of virus diseases in children. Public Health 2001; 115: 201207.
15.Nokes, DJ, et al. An evaluation of oral-fluid collection devices for the determination of rubella antibody status in a rural Ethiopian community. Transactions of the Royal Society of Tropical Medicine and Hygiene 1998; 92: 679685.
16.Rose, D, Pevalin, DA. A Researchers Guide to the National Statistics Socioeconomic Classification. London: Sage Publications, 2003.
17.Office for National Statistics. Ethnic group statistics. A guide for the collection and classification of ethnicity data. Technical report (http://www.statistics.gov.uk/about/ethnic_group_statistics/). Accessed 30 July 2007.
18.Zou, G. A modified Poisson regression approach to prospective studies with binary data. American Journal of Epidemiology 2004; 159: 702706.
19.Petersen, MR, Deddens, JA. A comparison of two methods for estimating prevalence ratios. BMC Medical Research Methodology 2008; 8: 9.
20.Stata Corporation. Survey Data Reference Manual Release 9. Stata Press, 2005.
21.Ochnio, JJ, et al. Participant-collected, mail-delivered oral fluid specimens can replace traditional serosurveys: a demonstration-of-feasibility survey of hepatitis A virus-specific antibodies in adults. Canadian Journal of Public Health 2007; 98: 3740.
22.Morris-Cunnington, MC, et al. A population-based seroprevalence study of Hepatitis A virus using oral fluid in England and Wales. American Journal of Epidemiology 2004; 159: 786794.
23.O'Connell, T, et al. Oral fluid collection by post for viral antibody testing. International Journal of Epidemiology 2001; 30: 298301.
24.Chatzipantazi, P, et al. The feasibility and acceptability of collecting oral fluid from healthy children for anti-HCV testing. Archives of Disease in Childhood 2004; 89: 185187.
25.Crowcroft, NS, et al. Epidemiology of Epstein-Barr virus infection in pre-adolescent children: application of a new salivary method in Edinburgh, Scotland. Journal of Epidemiology and Community Health 1998; 52: 101104.
26.Gibbs, S, et al. Atopic dermatitis and the hygiene hypothesis. International Journal of Epidemiology 2004; 33: 199207.
27.Rothmier, JD, Lasley, MV, Shapiro, G. Factors influencing parental consent in pediatric clinical research. Pediatrics 2003; 111; 10371041.
28.Tait, A, Voepel-Lewis, T, Malviya, S. Participation of children in clinical research: factors that influence a parent's decision to consent. Anesthesiology 2003; 99: 819825.
29.Sabri, AA, Qayyum, MA. Increasing the participation: another factor. PLoS Med 2005; 3: e250.
30.Hunt, SM, Bhopal, R. Self report in clinical and epidemiological studies with non-English speakers, the challenge of language and culture. Journal of Epidemiology and Community Health 2004; 58: 618622.
31.Hunt, S, Bhopal, R. Self reports in research with non-English speakers. British Medical Journal 2003; 327: 352353.
32.Bartlett, C, et al. The causes and effects of socio-demographic exclusions from clinical trials. Health Technology Assessment 2005; 9: 1152.
33.Hussain-Gambles, M, Atkin, K, Leese, B. Why ethnic minority groups are under-represented in clinical trials: a review of the literature. Health and Social Care in the Community 2004; 12: 382388.
34.Hussain-Gambles, M, et al. Involving South Asian patients in clinical trials. Health Technology Assessment 2004; 42: 1109.
35.Mason, S, et al. Representation of South Asian people in randomised clinical trials: analysis of trials' data. British Medical Journal 2003; 326: 12441245.
36.Chan, H, Moriarty, K. Are ethnic minorities excluded from clinical research? Bulletin of Medical Ethics. 2005; 211: 2224.
37.Lamvu, G, et al. Racial differences among reasons for participating in research of pregnancy outcomes: The right from the start experience Gender Medicine 2005; 2: 166173.
38.Crider, KS, et al. Racial and ethnic disparity in participation in DNA collection at the Atlanta Site of the National Birth Defects Prevention Study. American Journal of Epidemiology 2006; 164: 805812.
39.Shavers, VL, et al. Racial differences in factors that influence the willingness to participate in medical research studies. Annals of Epidemiology 2002; 12: 248256.
40.Sheikh, A. Why are ethnic minorities under-represented in US research studies? PLoS Med 2005; 3: e49.

Keywords

Feasibility of collecting oral fluid samples in the home setting to determine seroprevalence of infections in a large-scale cohort of preschool-aged children

  • S. E. BARTINGTON (a1), C. PECKHAM (a1), D. BROWN (a2), H. JOSHI (a3) and C. DEZATEUX (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed