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Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

  • P. B. Capstick (a1), A. J. M. Garland (a1), W. G. Chapman (a1) and R. C. Masters (a1)

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For maximum utilization of deep cultures to produce FMD virus it was important to have adequate control of culture temperature and pH. Culture temperature should be controlled within the range 34·25°7–35°C. and culture pH at 7·2. The culture system became less efficient as the cell concentration was increased from 1 × 106·0 to 9 × 106·0 cells/ml. A cell concentration of 2·5 × 106·0 cells/ml. represented a working compromise between efficiency and antigen titre/ml. for inactivated FMD vaccine production.

The input virus/cell ratio had no effect on the time or titre of peak virus yield in the range 1:1 to 1:320. This makes the production of seed virus from small numbers of monolayer cultures feasible and economical.

Virus yield was improved by the addition of 5% serum. It would be more satisfactory if a serum-free cell strain could be developed.

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References

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Bradish, C. J., Jowett, C. & Kirkham, J. B. (1964). The fixation of complement by virus antibody complexes. J. gen. Microbiol. 35, 27.
Brown, F. & Cartwright, B. (1960). Purification of the virus of foot-and-mouth disease by fluorocarbon treatment and its dissociation from neutralizing antibody. J. Immun. 85, 309.
Brown, F. & Newman, J. F. E. (1963). In vitro measurement of the potency of inactivated foot-and-mouth disease virus vaccines. J. Hyg., Camb. 61, 345.
Capstick, P. B., Telling, R. C., Chapman, W. G. & Stewart, D. L. (1962). Growth of a cloned strain of hamster kidney cells in suspended culture and their susceptibility to the virus of foot-and-mouth disease. Nature, Lond. 195, 1163.
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Telling, R. C. & Elsworth, R. (1965). Submerged culture of hamster kidney cells in a stainless steel vessel. Biotech. Bioengng. 7, 417.
Telling, R. C., Radlett, P. & Mowat, G. N. (1967). Production of Semliki Forest virus from hamster kidney cells in deep culture vessels. Biotech. Bioengng. 9, 257.
Telling, R. C. & Stone, C. J. (1964). A method of automatic pH control of a bicarbonate/CO2 buffer system for the submerged culture of hamster kidney cells. Biotech. Bioengng. 6, 147.

Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

  • P. B. Capstick (a1), A. J. M. Garland (a1), W. G. Chapman (a1) and R. C. Masters (a1)

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