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Extended phage-typing scheme for Campylobacter jejuni and Campylobacter coli

Published online by Cambridge University Press:  15 May 2009

R. Khakhria
Affiliation:
National Reference Service for Campylobacters, National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario KIA 0L2, Canada
H. Lior
Affiliation:
National Reference Service for Campylobacters, National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario KIA 0L2, Canada
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The extended phage-typing scheme described for Campylobacter jejuni and Campylobacter coli has established 46 different phage types using 19 typing phages. Altogether 754 campylobacter isolates, 672 C. jejuni and 82 C. coli, isolated from human and non-human sources received from 17 different countries were phage-typed. Overall, 80·6% of the total isolates were typable. Among typable strains, 9 phage types (3, 5, 10, 11, 18, 19, 23, 26 and 44) represented 57·0% of the strains, 21·3% of the strains belonged to another 37 phage types and the remaining 2·3% of isolates were designated atypical. The most common phage type 11 (140/754) was frequently observed among C. jejuni isolates from human (113/561) and non-human sources (18/111). whereas type 44 was frequent among C. coli isolates from human (22/59) and from non-human sources (8/23). A study of the animal host-associations of common phage types showed that contaminated cattle and poultry appear to be the most common sources of human infection. The greatest variety of phage types was observed in Canada (24 phage types), followed by Portugal (17 types) and the UK (14 types), reflecting the larger sample sizes from these countries. Phage type 11 was encountered in 12 different countries and prevalence of other phage types varied from one country to another. The number of isolates typable with the scheme varied from 93·2% (261/280) in Canada to 61% (47/77) in Thailand. However, the number and diversity of phage types makes phage typing the method of choice in epidemiological studies of campylobacter infections.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Butzler, JP, Skirrow, MB. Campylobacter enteritis. Clin Gastroenterol 1979; 8: 737–65.Google Scholar
2.Blaser, MJ, Taylor, MJ, Feldman, RA. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev 1983; 5: 157–76.Google Scholar
3.Lior, H, Woodward, DL, Edgar, JA, Laroche, LJ, Gill, P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol 1982; 15: 761–8.Google Scholar
4.Lior, H. Extended biotyping scheme for Campylobacter jejuni. J Clin Microbiol 1984; 20: 636–40.CrossRefGoogle ScholarPubMed
5.Figueroa, G, Troncoso, M, Galeno, H, Soto, V, Toledo, MS. Biotypes, serogroups and antibiotic susceptibility of Campylobacter jejuni and Campylobacter coli in Chile. J Infect 1990; 20: 123–7.CrossRefGoogle ScholarPubMed
6.Hirschl, AM, Lior, H, Wolf, D et al. , Occurrence, serotypes and biotypes of thermophilic Campylobacter isolated in Vienna. Zbl Bakt Hyg 1987; A 266: 94103.Google ScholarPubMed
7.Bar, W, Fricke, G, Goosens, H. Distribution of serotypes and biotypes of thermophilic Campylobacters in the Federal Republic of Germany: a comparison with other countries. Zbl Bakt 1989; 271: 127–34.Google Scholar
8.Grajewski, BA, Kusek, JW, Gelfand, HM. Development of a bacteriophage typing system for Campylobacter jejuni and Campylobacter coli. J. Clin Microbiol 1985; 22: 13–8.CrossRefGoogle ScholarPubMed
9.Salama, SM, Bolton, FJ, Hutchinson, DN. Application of a new phage-typing scheme to campylobacters isolated during outbreaks. Epidemiol Infect 1990; 104: 405–11.CrossRefGoogle ScholarPubMed
10.Salama, SM, Bolton, FJ, Hutchinson, DN. Improved method for the isolation of Campylobacter jejuni and Campylobacter coli bacteriophages. Lett Appl Microbiol 1989; 8: 57.Google Scholar
11.Khakhria, R, Lior, H. Phagetyping of Campylobacter jejuni. In: Kaijser, B, Falsen, E, eds. Campylobacter IV. Goterna, Kungalu, Sweden, 1988: 134.Google Scholar
12.Lior, H, Woodward, D, Khakhria, R. Characterization of Campylobacter jejuni outbreaks by 3 epidemiological typing markers. In: Kaijser, B, Falsen, E, eds. Campylobacter IV. Goterna, Kungalu, Sweden, 1988: 103–4.Google Scholar
13.Callow, BR. A new phage typing scheme for Salmonella typhimurium. J Hyg 1959; 57: 346–59.Google Scholar
14.Adams, MH. Bacteriophages. New York: Interscience Publishers Inc., 1959.CrossRefGoogle Scholar
15.Farmer, JJ III, Hickman, FW, Sikes, JV. Automation of Salmonella typhi phage typing. Lancet 1975; ii: 787–90.CrossRefGoogle Scholar
16.Anderson, ES, Williams, REO. Bacteriophage typing of enteric pathogens and staphylococci and its use in epidemiology. J Clin Pathol 1956; 9: 94114.Google Scholar
17.Lior, H. LesCampylobacter Marqueurs Epidémiologiques. Méd Malad Infect 1989; 19: special Mars 1824.Google Scholar
18.Patton, CM, Wachsmuth, IK, Evins, GM et al. , Evaluation of 10 methods to distinguish epidemic-associated Campylobacter strains. J Clin Microbiol 1991; 29: 680–8.Google Scholar
19.Cabrita, J. Contribuição para o estudo da bioecologia do Campylobacter Enteropatogénico. [Thesis] Lisbon, Portugal: Universidade de Lisboa, 1989: 138–50.Google Scholar