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Epidemiology of carbapenem-resistant Escherichia coli and first report of blaVIM carbapenemases gene in calves from India

  • M. Senthil Murugan (a1), D. K. Sinha (a1), O. R. Vinodh Kumar (a1), Ajay Kumar Yadav (a1), B. S. Pruthvishree (a1), Prasanna Vadhana (a2), K. R. Nirupama (a1), Monika Bhardwaj (a2) and B. R. Singh (a1)...

Abstract

A cross-sectional study on six dairy farms was conducted to ascertain the occurrence of carbapenem-resistant Escherichia coli in calves. Two-hundred and seventy-nine isolates of E. coli were recovered from 90 faecal samples from apparently healthy (45) and diarrhoeal (45) calves. The isolates were screened for phenotypic susceptibility to carbapenems and production of metallo β-lactamase, as well as five carbapenemase resistance genes by PCR, and overexpression of efflux pumps. Eighty-one isolates (29.03%) were resistant to at least one of three carbapenem antibiotics [meropenem (23.30%), imipenem (2.15%) and ertapenem (1.43%)], and one isolate was positive for the blaVIM gene which was located on an Incl1 plasmid of a novel sequence type (ST 297) by multilocus sequence typing. The majority (83.95%) of isolates had an active efflux pump. Calves housed on concrete floors were approximately seven times more likely to acquire meropenem-resistant isolates than those housed on earthen floors (95% CI 1.27–41.54). In India, carbapenem drugs are not used in food animal treatment, hence carbapenem-resistant strains in calves possibly originate from the natural environment or human contact and is of public health importance. To our knowledge, this is the first report of blaVIM carbapenemases gene in calves from India.

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Copyright

This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike licence (http://creativecommons.org/licenses/by-nc-sa/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the same Creative Commons licence is included and the original work is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use.

Corresponding author

Author for correspondence: O. R. Vinodh Kumar, E-mail: vinodhkumar.rajendran@gmail.com

References

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1.Nordmann, P et al. (2011) The emerging NDM carbapenemases. Trends in Microbiology 19, 588595.
2.Bhardwaj, M et al. (2015) Emergence of carbapenemase producing pathogens in animals. Pharmaceutica Analytica Acta 6, 16.
3.Muller, A et al. (2018) Higher third-generation cephalosporin prescription proportion is associated with lower probability of reducing carbapenem use: a nationwide retrospective study. Antimicrobial Resistance & Infection Control 7, 110.
4.Kelly, AM, Mathema, B and Larson, EL (2017) Carbapenem-resistant Enterobacteriaceae in the community: a scoping review. International Journal of Antimicrobial Agents 50, 127134.
5.Picao, RC, Cardoso, JP and Campana, EH (2013) The route of antimicrobial resistance from the hospital effluent to the environment: focus on the occurrence of KPC producing Aeromonas spp., and Enterobacteriaceae in sewage. Diagnostic Microbiology and Infectious Disease 76, 8085.
6.Castanheira, M et al. (2011) Early dissemination of NDM-1- and OXA-181-producing Enterobacteriaceae in Indian hospitals: report from the SENTRY Antimicrobial Surveillance Program. 2006–2007. Antimicrobial Agents and Chemotherapy 55, 12741278.
7.Page, SW and Gautier, P (2012) Use of antimicrobial agents in livestock. Revue Scientifique et Technique – OIE 31, 145188.
8.Clinical and Laboratory Standards Institute (CLSI) (2014). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement 2014. M100-S24. Wayne, PA, USA: CLSI, Vol 34, pp. 5660.
9.Pruthvishree, BS et al. (2017) Spatial molecular epidemiology of carbapenem-resistant and New Delhi metallo beta-lactamase (blaNDM)-producing Escherichia coli in the piglets of organized farms in India. Journal of Applied Microbiology 122, 15371546.
10.Poirel, L et al. (2011) Multiplex PCR for detection of acquired carbapenemase genes. Diagnostic Microbiology and Infectious Disease 70, 119123.
11.Doyle, D et al. (2012) Laboratory detection of Enterobacteriaceae that produce carbapenemases. Journal of Clinical Microbiology 50, 38773880.
12.Johnson, TJ et al. (2007) Plasmid replicon typing of commensal and pathogenic E. coli isolates. Applied and Environmental Microbiology 15, 19761983.
13.Martins, M et al. (2013) A simple method for assessment of MDR bacteria for over-expressed efflux pumps. The Open Microbiology Journal 7, 7282.
14.Gandra, S et al. (2016) Faropenem consumption is increasing in India. Clinical Infectious Diseases 62, 10501052.
15.Falagas, ME, Karageorgopoulos, DE and Nordmann, P (2011) Therapeutic options for infections with Enterobacteriaceae producing carbapenem-hydrolyzing enzymes. Future Microbiology 6, 653666.
16.Rogers, BA, Sidjabat, HE and Silvey, A (2013) Treatment options for New Delhi metallo-beta-lactamase-harboring enterobacteriaceae. Microbial Drug Resistance 19, 100103.
17.Fischer, J et al. (2012) Escherichia coli producing VIM-1 carbapenemase isolated on a pig farm. Journal of Antimicrobial Chemotherapy 67, 17931795.
18.Nirupama, KR et al. (2018) Molecular characterization of blaOXA-48 carbapenemase, extended-spectrum β-lactamase-and Shiga toxin-producing Escherichia coli isolated from farm piglets in India. Journal of Global Antimicrobial Resistance 13, 201205.
19.Ghatak, S et al. (2013) Detection of New Delhi metallo beta-lactamase and extended- spectrum beta-lactamase genes in Escherichia coli isolated from mastitic milk samples. Transboundary and Emerging Disease 60, 385389.
20.de Verdier, K et al. (2012) Antimicrobial resistance and virulence factors in E. coli from Swedish dairy calves. Acta Veterinaria Scandinavica 54, 110.
21.Samanta, I et al. (2015) Approaches to characterize extended spectrum beta-lactamase/beta-lactamase producing Escherichia coli in healthy organized vis-a-vis backyard farmed pigs in India. Infection, Genetics and Evolution 36, 224230.
22.Martins, M, Viveiros, M and Couto, I (2011) Identification of efflux pump-mediated multidrug-resistant bacteria by the ethidium bromide-agar cartwheel method. In Vivo 25, 171178.
23.Bendali, F et al. (1999) Risk factors associated with diarrhoea in newborn calves. Veterinary Research 30, 509522.
24.Gullikse, SM et al. (2009) Enteropathogens and risk factors for diarrhea in Norwegian dairy calves. Journal of Dairy Science 92, 50575066.
25.Patel, G et al. (2008) Outcomes of carbapenem-resistant Klebsiella pneumoniae infection and the impact of antimicrobial and adjunctive therapies. Infection Control & Hospital Epidemiology 29, 10991106.
26.Davies, J and Davies, D (2010) Origins and evolution of antibiotic resistance. Microbiology and Molecular Biology Reviews 74, 417433.

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