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Emergence of dengue virus type 4 (genotype I) in India

  • P. K. DASH (a1), S. SHARMA (a1), A. SRIVASTAVA (a1), S. R. SANTHOSH (a1), M. M. PARIDA (a1), M. NEERAJA (a2), M. V. S. SUBBALAXMI (a2), V. LAKSHMI (a2) and P. V. L. RAO (a1)...

Summary

Dengue is an emerging arboviral disease and currently poses the greatest arboviral threat to human health. In recent decades, there has been a substantial increase in dengue outbreaks in many parts of the world including India. We performed an in-depth investigation of a major dengue outbreak in Andhra Pradesh, southern India in 2007 by serology, virus isolation, RT–PCR and genotyping. The results revealed an unusual emergence of dengue virus type 4 (DENV-4) along with the prevailing DENV-3. Phylogenetic analysis based on complete envelope gene of 182 globally diverse DENV-4 isolates demonstrated the involvement of a unique clade of genotype I of DENV-4 in the outbreak. This study also demonstrated a clear shift in the dominant serotype from DENV-3 to DENV-4 in India. This is the first report regarding the molecular characterization of Indian isolates of DENV-4, which has the potential to be involved in future outbreaks.

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Copyright

Corresponding author

*Author for correspondence: Dr P. K. Dash, Division of Virology, Defence R & D Establishment, Jhansi Road, Gwalior – 474 002, MP, India. (Email: pabandash@rediffmail.com)

References

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1.WHO. Dengue fact sheet (http://www.who.int/mediacentre/factsheets/fs117/en/). Accessed 16 March 2010.
2.Gubler, DJ. Dengue and dengue hemorrhagic fever. Clinical Microbiology Review 1998; 11: 480496.
3.Dar, L, et al. The first major outbreak of DHF in Delhi, India. Emerging Infectious Diseases 1999; 5: 589590.
4.Dash, PK, et al. Emergence of dengue virus type-3 in northern India. Southeast Asian Journal of Tropical Medicine and Public Health 2005; 36: 2532.
5.Dar, L, et al. Cocirculation of dengue serotypes, Delhi, India, 2003. Emerging Infectious Diseases 2006; 12: 352353.
6.Kukreti, H, et al. Phylogenetic studies reveal existence of multiple lineages of a single genotype of DENV-1 (genotype III) in India during 1956–2007. Virology Journal 2009; 6: 1.
7.National Institute of Virology, Pune, India. Dengue (DEN) brochure. (http://www.icmr.nic.in/pinstitute/niv/DENGUE.pdf). Accessed 16 March 2010.
8.Lanciotti, RS, et al. Rapid selection and typing of dengue viruses from clinical samples by using reverse transcriptase chain reaction. Journal of Clinical Microbiology 1992; 30: 545551.
9.Dash, PK, et al. Reemergence of dengue virus type-3 (subtype-III) in India: implications for increased incidences of DHF and DSS. Virology Journal 2006; 3: 55.
10.Klungthong, C, et al. The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand. Virology 2004; 329: 168179.
11.Ronquist, F, Huelsenbeck, JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003; 19: 15721574.
12.Kumar, S, Tamura, K, Nei, M. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 2004; 5: 150163.
13.Gould, EA, Clegg, JCS. Growth, titration and purification of togaviruses. In: Mahy, BVJ, ed. Virology: A Practical Approach. Oxford: Oxford University Press, 1986, pp. 4388.
14.Lanciotti, RS, Gubler, DJ, Trent, DW. Molecular evolution and phylogeny of dengue 4 viruses. Journal of General Virology 1997; 78: 22792286.
15.Ito, M, et al. Phylogenetic analysis of dengue viruses isolated from imported dengue patients: possible aid for determining the countries where infections occurred. Journal of Travel Medicine 2007; 14: 233244.
16.Rico-Hesse, R, et al. Origins of dengue type-2 viruses associated with increased pathogenicity in the Americas. Virology 1997; 230: 244251.
17.Nisalak, A, et al. Serotype-specific dengue virus circulation and dengue disease in Bangkok, Thailand from 1973 to 1999. American Journal of Tropical Medicine and Hygiene 2003; 68: 191202.
18.Carrington, CVF, et al. Invasion and maintenance of dengue virus type 2 and type 4 in the Americas. Journal of Virology 2005; 79: 1468014687.

Keywords

Emergence of dengue virus type 4 (genotype I) in India

  • P. K. DASH (a1), S. SHARMA (a1), A. SRIVASTAVA (a1), S. R. SANTHOSH (a1), M. M. PARIDA (a1), M. NEERAJA (a2), M. V. S. SUBBALAXMI (a2), V. LAKSHMI (a2) and P. V. L. RAO (a1)...

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