Skip to main content Accessibility help
×
Home

Effect of sub-lethal treatment with formalin on the germination of Aspergillus fumigatus spores

  • G. R. Smith (a1)

Summary

Sub-lethal exposure of Aspergillus fumigatus spore suspensions to formalin resulted in prolongation by 1–22 days of the period of less than one day normally needed by spores to produce visible growth in Sabouraud's liquid medium at 37° C.; the degree of delay depended on the concentration of formalin and the duration of exposure, and was due to an increase in the germination-time of spores. The formalin concentration could be adjusted so as to affect the germination-time of almost all spores in a suspension without reducing viability. The effect on germination was not abolished by thorough washing or treatment with sodium sulphite. The spores of four different strains of A. fumigatus and of cultures aged 3 to 14 days reacted similarly to formalin treatment. Although of greatly reduced virulence for mice, affected viable spores were still capable of producing infection and death following intravenous inoculation, provided they were not eliminated by the host before germination occurred.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effect of sub-lethal treatment with formalin on the germination of Aspergillus fumigatus spores
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Effect of sub-lethal treatment with formalin on the germination of Aspergillus fumigatus spores
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Effect of sub-lethal treatment with formalin on the germination of Aspergillus fumigatus spores
      Available formats
      ×

Copyright

References

Hide All
Byrde, R. J. W. (1966). The vulnerability of fungus spores to fungicides. In The Fungus Spore, Proceedings of the eighteenth symposium of the Colston Research Society (ed. Madelin, M. F.), p. 289. London: Butterworths.
Englesberg, E. (1952). The mutagenic action of formaldehyde on bacteria. Journal of Bacteriology 63, 1.
Fildes, P. (1940). The mechanism of the anti-bacterial action of mercury. British Journal of Experimental Pathology 21, 67.
Gegenbauer, V. (1921). Studien über die Desinfektionswirkung wässeriger Formaldehydlösungen. Archiv für Hygiene 90, 239.
Hailer, E. (1921 a). Versuche über die Beziehung zwischen Formaldehyd und der Bakterienund Sporenzelle. Biochemische Zeitschrift 125, 69.
Hailer, E. (1921 b). Die baktericide Nachwirkung von Formaldehydlösungen. Biochemische Zeitschrift 125, 84.
McCallan, S. E. A. (1930). Studies of fungicides II. Testing protective fungicidesin the laboratory. Memoirs. Cornell University Agricultural Experiment Station 128, 8.
Miller, L. P., McCallan, S. E. A. & Weed, R. M. (1953). Rate of uptake and toxic dose on a spore weight basis of various fungicides. Contributions. Boyce Thompson Institute for Plant Research 17, 173.
Müller, A. (1920). Die Resistenz der Milzbrandsporen gegen Chlor, Pickelfiussigkeit, Formaldehyd und Sublimat. Archiv für Hygiene 89, 363.
Müller, E. & Biedermann, W. (1952). Der Einfiuss von Cu+6-Ionen auf den Keimungsablauf von Alternaria tenuis. Phytopathologische Zeitschrift 19, 343.
Nash, T. & Hirch, A. (1954). The revival of formaldehyde-treated bacteria. Journal of Applied Chemistry, London 4, 458.
Nordgren, G. (1939). Investigations on the sterilization efficacy of gaseous formaldehyde. Acta pathologica et microbiologica scandinavica. Supplementum XL.
Report (1956). Disinfection of fabrics with gaseous formadehyde. Report by the Committee on Formaldehyde Disinfection of the Public Health Laboratory Service. Journal of Hygiene 56, 488.
Ross, A. F. & Stanley, W. M. (1938). The partial reactivation of formolized tobacco mosaic virus protein. Journal of General Physiology 22, 165.
Schultz, E. W. & Gebhardt, L. P. (1935). Nature of formalin inactivation of bacteriophage. Proceedings of the Society for Experimental Biology and Medicine 32, 1111.
Smith, G. R. (1972). Experimental aspergillosis in mice: aspects of resistance. Journal of Hygiene 70, 741.
Somers, E. (1966). The sites of reaction of fungicides in spores. In The Fungus Spore, Proceedings of the eighteenth symposium of the Colston Research Society (ed. Madelin, M. F.), p. 299. London: Butterworths.
Williams, R. E. O., Blowers, R., Garrod, L. P. & Shooter, R. A. (1966). In Hospital Infection. Causes and Prevention, 2nd ed.London: Lloyd-Luke (Medical Books) Ltd.

Effect of sub-lethal treatment with formalin on the germination of Aspergillus fumigatus spores

  • G. R. Smith (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed