Skip to main content Accessibility help
×
Home

Effect of stool storage at room temperature on salmonella isolation from faeces

  • R. W. S. Harvey and T. H. Price (a1)

Summary

Stools, known to have contained salmonellas were cultured in three enrichment media on arrival at the laboratory. The three media were laboratory prepared selenite F, laboratory prepared Muller-Kauffmann tetrathionate and commercially obtained Oxoid Muller-Kauffmann tetrathionate (CM343). Where faecal samples were sufficiently large, they were stored at room temperature and re-examined using the same three enrichment broths. Whether fresh or stored stools were investigated, the laboratory prepared media were significantly more efficient than the commercial medium. In a straight comparison of the two laboratory prepared media, no significant difference in efficiency was evident with fresh stool samples. With stored specimens, however, Muller-Kauffmann tetrathionate was significantly better than selenite F. This finding might be relevant in examining samples delayed in the post.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effect of stool storage at room temperature on salmonella isolation from faeces
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Effect of stool storage at room temperature on salmonella isolation from faeces
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Effect of stool storage at room temperature on salmonella isolation from faeces
      Available formats
      ×

Copyright

References

Hide All
Anon (1970). Can bacteriologist agree? Lancet i, 664.
Anon (1975). Meat and meat products – detection of salmonellae(reference method). International Standard ISO 3565–1975 (E). Geneva: International Organization for Standardization.
Edel, W. & Kampelmacheu, E. H. (1969). Salmonella isolation in nine European laboratories using a standardized technique. Bulletin of the World Health Organization 41, 297300.
Harvey, R. W. S. (1956). Choice of a selective medium for the routine isolation of members of the salmonella group. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 15, 118124.
Harvey, R. W. S. & Price, T. H. (1975). Studies on the isolation of Salmonella dublin. Journal of Hygiene 74, 369374.
Harvey, R. W. S. & Price, T. H. (1977). Observations on pre-enrichment for isolating Saltnonellas from sewage polluted natural water using Muller-Kauffmann tetrathionate broth prepared with fresh and desiccated ox bile. Journal of Applied Bacteriology 43, 145148.
Harvey, R. W. S. & Price, T. H. (1980). Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios. Journal of Hygiene 85, 125128.
Harvey, R. W. S. & Price, T. H. (1982 a). A comparison of the R25 modification of Rappaport's enrichment medium with strontium chloride B for salmonella isolation from sewage polluted natural water. Journal of Hygiene 89, 111117.
Harvey, R. W. S. & Price, T. H. (1982 b). Salmonella isolation and identification techniques alternative to the standard method. In Isolation and Identification Methods fur Food Poisoning Organisms, p. 51. Society for Applied Bacteriology Technical Series, no. 17 (ed. Corry, , Roberts, and Skinner, ). Academic Press: London.
Harvey, R. W. S., Price, T. H. & Crone, P. B. (1975). Quality control tests of two salmonella enrichment media using different inocula. Journal of Hygiene 74, 375384.
Harvey, R. W. S., Price, T. H. & Xirouchaki, E. (1979). Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. Journal of Hygiene 83, 451460.
Harvey, R. W. S. & Thomson, S. (1953). Optimum temperature of incubation for isolation of salmonellae. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 12, 149150.
Iveson, J. B. & Mackay-Scollay, E. M. (1969). Strontium chloride and strontium selenite enrichment media in the isolation of Salmonella. Journal of Hygiene 67, 457464
Kauffmann, F. (1930). Ein kombiniertes Anreicherungsverfahren fur Typhus-und Paratyphusbazillen. Zentralblaft fur Bakteriologie, Parasitenkunde und Infektionskrankheiten I. Abt. Orig. 119, 148152.
Kauffmann, F. (1935). Weitere Erfahrungen mit kombinierten Anreicherungsverfahren für Salmonella bazillen. Zeitschrift für Hygiene und Infektionskrankheiten 117, 2632.
Leifson, E. (1936). New selenite enrichment media for the isolation of typhoid and paratyphoid (salmonella) bacilli. American Journal of Hygiene 24, 423432.
McCov, J. H. (1975). Trends in salmonella food poisoning in England and Wales 1941–72. Journal of Hygiene 74, 271282.
Muller, L. (1923). Un nouveau milieu d'enrichissement pour la recherche du bacille typhique et des paratyphiques. Comptes liendus des Seances de la Societe de Biologie et des ses Filiales 89, 434437.
Price, T. H. (1976). Isolation of Shigella sonnei by fluid media. Journal of Hygiene 77, 341348.
Read, R. B. & Reyes, A. L. (1968). Variation in plating efficiency of salmonellae on eight lots of brilliant green agar. Applied Microbiology 16, 740748.
Roberts, D., Boag, K., Hall, M. L. M. & Ship, C. R. (1975). The isolation of salmonellas from British pork sausage meat. Journal of Hygiene 75, 173184.
Stokes, E. J. (1978). Quality control in the bacteriological laboratory. Health Care World Wide. Intake Issue No. 58, 1. Abbott Laboratories Ltd.
Taylor, W. I. & Schelhart, D. (1968). Isolation of shigellae. V. Comparison of enrichment broths with stools. Addendum. Applied Microbiology 16, 13831386.
Thomson, S. (1955). The numbers of pathogenic bacilli in faeces in intestinal diseases. Journal of Hygiene 52, 217224.
Van Schothorst, M., Van Leusden, M., Jeunink, J. & De Dreu, J. (1977). Studies on the multiplication of salmonellae in various enrichment media at different incubation temperatures. Journal of Applied Bacteriology 42, 157163.
Vassiliadis, P., Pateraki, E., Papadakis, J. & Trichopoulos, D. (1974). Evaluation of the growth of salmonellae in Rappaport's broth and in Muller-Kauffmann's tetrathionate broth. Journal of Applied Bacteriology 37, 411418.

Effect of stool storage at room temperature on salmonella isolation from faeces

  • R. W. S. Harvey and T. H. Price (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed