Hostname: page-component-7479d7b7d-qs9v7 Total loading time: 0 Render date: 2024-07-12T07:14:59.225Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA typing of epidemiologically-related isolates of Aspergillus fumigatus*

Published online by Cambridge University Press:  15 May 2009

M. Birch ,
N. Nolard ,
G. S. Shankland  and
D. W. Denning
M. Birch
Affiliation:
University of ManchesterDepartment of Medicine, Hope Hospital, Salford M6 8HD
N. Nolard
Affiliation:
Institute of Hygiene and Epidemiology, Section of Mycology, Rue Juliette Wytsman 14, B 1050 Brussels, Belgium
G. S. Shankland
Affiliation:
Regional Mycology Reference Laboratory, Department of Dermatology, Robertson Building, 54 Dumbarton Road, Glasgow G11 6AQ
D. W. Denning*
Affiliation:
University of ManchesterDepartment of Medicine, Hope Hospital, Salford M6 8HD Department of Infectious Diseases & Tropical Medicine (Monsall Unit), North Manchester General Hospital, Delaunays Road, Crumpsall, University of Manchester, Manchester M8 6RB, UK
*
† Author for correspondence and reprint requests.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Invasive aspergillosis is often nosocomially acquired and carries a high mortality. Molecular typing methods to discriminate isolates have now been developed. Using simple restriction endonuclease (Sal1 and Xho1) digestion of total genomic DNA, we have typed 25 epidemiologically-related isolates of A. fumigatus from six hospital episodes of invasive aspergillosis. Eight DNA types were found and in each case the DNA type matched precisely the epidemiological data. Thus DNA typing of A. fumigatus can provide the means to match isolates from linked sources and distinguish isolates from diverse origins.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 161 - 168
Copyright
Copyright © Cambridge University Press 1995

Footnotes

*

This work was presented, in part, as an abstract (No 1162) at the 6th European Congress of Infectious Diseases, 28–31 March 1993, Seville, Spain.

References

REFERENCES

1.Bardana, EJ. The clinical spectrum of aspergillosis. Part 1. Epidemiology, pathogenicity, infection in animals and immunology of Aspergillus. CRC Grit Rev Lab Sci 1980; 13: 21–83.Google Scholar
2.Barnes, AJ, Denning, DW. Aspergillus – significance as a pathogen. Rev Med Microbiol 1993; 4: 176–80.Google Scholar
3.Denning, DW, Follansbee, S, Scolaro, M, Norris, S, Edelstein, D, Stevens, DA. Pulmonary aspergillosis in AIDS. N Engl J Med 1991; 324: 654–62.Google Scholar
4.Nolard, N, Detandt, M, Beguin, H. Ecology of Aspergillus in the human environment. In: Vanden, Bossche H, Mackenzie, D, Cauwenbergh, G, eds. Aspergillus and aspergillosis. New York: Plenum Press, 1984: 309–15.Google Scholar
5.Fanti, F, Conti, S, Campani, L, Morace, G, Dettori, G, Polonelli, L. Studies on the epidemiology of Aspergillus fumigatus in a university hospital. Eur J Epidemiol 1989; 5: 8–14.CrossRefGoogle ScholarPubMed
6.Walsh, TJ, Dixon, DM. Nosocomial aspergillosis: Environmental microbiology, hospital epidemiology, diagnosis and treatment. Eur J Epidemiol 1989; 5: 131–42.Google Scholar
7.Summerbell, RC, Krajden, S, Kane, J. Potted plants in hospitals as reservoirs of pathogenic fungi. Mycopathologia 1989; 106: 13–22.CrossRefGoogle ScholarPubMed
8.Denning, DW, Clemons, KV, Hanson, LH, Stevens, DA. Restriction endonuclease analysis of total cellular DNA of Aspergillus fumigatus isolates of geographically and epidemiologically diverse origins. J Infect Dis 1990; 162: 1151–8.Google Scholar
9.Burnie, JP, Mathews, RC, Clark, I, Milne, LFR. Immunoblot fingerprinting Aspergillus fumigatus. J Immunol Methods 1989; 118: 179–86.Google Scholar
10.Burnie, JP, Coke, A, Matthews, RC. Restriction endonuclease analysis of Aspergillus fumigatus. J Clin Pathol 1992; 5: 324–7.CrossRefGoogle Scholar
11.Denning, DW, Shankland, GS, Stevens, DA. DNA fingerprinting of Aspergillus fumigatus isolates from patients with aspergilloma. J Med Vet Mycol 1991; 29: 339–42.Google Scholar
12.Aufauvre-Brown, A, Cohen, J, Holden, DW. Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus. J Clin Microbiol 1992; 30: 2991–3.CrossRefGoogle ScholarPubMed
13.Giradin, H, Latge, JP, Thyagarajan, S, Morrow, B, Soil, DR. Development of DNA probes for fingerprinting Aspergillus fumigatus. J Clin Microbiol 1993; 31: 1547–54.Google Scholar
14.Plikaytis, BD, Carlone, GM, Edmonds, P, Mayer, LW. Robust estimation of standard curves for protein weight and linear duplex DNA base pair number after gel electrophoresis. Anal Biochem 1986; 152: 346–364.Google Scholar
15.Tait, RC, O'Driscoll, BR, Denning, DW. Unilateral wheeze due to pseudomembranous aspergillus tracheobronchitis in the immunocompromised patient. Thorax 1993; 48: 1285–7.CrossRefGoogle ScholarPubMed
16.Williams, JGK, Kubelik, AR, Livak, KJ, Rafalski, JA, Tingey, SV. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl Acid Res 1990; 18: 6531–5.CrossRefGoogle ScholarPubMed
17.Symoens, F, Viviani, MA, Nolard, N. Typing by immunoblot of Aspergillus fumigatus from nosocomial infections. Mycoses 1993; 36: 229–37.Google Scholar