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Distribution of molecular subtypes within Salmonella enterica serotype Enteritidis phage type 4 and S. Typhimurium definitive phage type 104 in nine European countries, 2000–2004: results of an international multi-centre study

Published online by Cambridge University Press:  25 January 2006

A. J. GATTO
Affiliation:
Health Protection Agency Centre for Infections, London, UK
T. M. PETERS
Affiliation:
Health Protection Agency Centre for Infections, London, UK
J. GREEN
Affiliation:
Health Protection Agency Centre for Infections, London, UK
I. S. T. FISHER
Affiliation:
Health Protection Agency Centre for Infections, London, UK
O. N. GILL
Affiliation:
Health Protection Agency Centre for Infections, London, UK
S. J. O'BRIEN
Affiliation:
Health Protection Agency Centre for Infections, London, UK
C. MAGUIRE
Affiliation:
Health Protection Agency Centre for Infections, London, UK
C. BERGHOLD
Affiliation:
Austrian Agency for Health and Food Safety, Graz, Austria
I. LEDERER
Affiliation:
Austrian Agency for Health and Food Safety, Graz, Austria
P. GERNER-SMIDT
Affiliation:
Statens Seruminstitut, Copenhagen, Denmark
M. TORPDAHL
Affiliation:
Statens Seruminstitut, Copenhagen, Denmark
A. SIITONEN
Affiliation:
National Public Health Institute, Helsinki, Finland
S. LUKINMAA
Affiliation:
National Public Health Institute, Helsinki, Finland
H. TSCHÄPE
Affiliation:
Robert Koch-Institut, Wernigerode, Germany
R. PRAGER
Affiliation:
Robert Koch-Institut, Wernigerode, Germany
I. LUZZI
Affiliation:
Istituto Superiore di Sanità, Rome, Italy
A. M. DIONISI
Affiliation:
Istituto Superiore di Sanità, Rome, Italy
W. K. VAN DER ZWALUW
Affiliation:
National Institute of Public Health & the Environment, Bilthoven, The Netherlands
M. HECK
Affiliation:
National Institute of Public Health & the Environment, Bilthoven, The Netherlands
J. COIA
Affiliation:
Scottish Salmonella Reference Laboratory, Glasgow, Scotland
D. BROWN
Affiliation:
Scottish Salmonella Reference Laboratory, Glasgow, Scotland
M. USERA
Affiliation:
Instituto de Salud Carlos III, Madrid, Spain
A. ECHEITA
Affiliation:
Instituto de Salud Carlos III, Madrid, Spain
E. J. THRELFALL
Affiliation:
Health Protection Agency Centre for Infections, London, UK
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Abstract

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This study investigates the distribution of pulsed-field gel electrophoresis (PFGE) profiles within Salmonella enterica serotype Enteritidis phage type (PT) 4 and S. Typhimurium definitive phage type (DT) 104, from cases of human infection in nine European countries from 2000 to 2004. Isolates were subtyped using standardized methods and gel images submitted by each participating country to the coordinating centre (Health Protection Agency Centre for Infections, London, UK), where they were entered into a central database, developed within BioNumerics software, and designated using an agreed nomenclature. S. Enteritidis PT4 (n=3637) was differentiated into 38 different profiles. Simpson's index of diversity (D) of profiles ranged from 0·2 to 0·4. Profile SENTXB.0001 represented at least 80% of all profiles in each country. S. Typhimurium DT104 (n=1202) was differentiated into 28 different profile types. Simpson's D was at least 0·6 in all countries except in Austria and Italy. In both these countries over 74% of S. Typhimurium DT104 profiles were STYMXB.0013. Profile STYMXB.0061, was predominant in Denmark, Spain, Finland and England & Wales where it represented between 36% and 45% of profiles. Profile STYMXB.0001 represented nearly half of all profiles in Scotland and 23% in England & Wales. PFGE is proving useful for further discrimination within S. Enteritidis PT4 and S. Typhimurium DT104. Ascertainment of international outbreaks involving common serotypes and phage types may be increased by the timely pooling of PFGE profiles within a central database readily accessible to all participating countries.

Type
Research Article
Copyright
2006 Cambridge University Press