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Disentangling outbreaks using whole-genome sequencing: concurrent multistate outbreaks of Salmonella Kottbus in Germany, 2017

  • J. Enkelmann (a1) (a2) (a3), A. von Laer (a1) (a2) (a3), S. Simon (a4), A. Fruth (a4), R. Lachmann (a1), K. Michaelis (a1), M. Borowiak (a5), S. Gillesberg Lassen (a6) and C. Frank (a1)...

Abstract

In June 2017, an outbreak of Salmonella Kottbus infection was suspected in Germany. We investigated the outbreak with whole-genome sequencing (WGS) and a case–control study. Forty-six isolates from 69 cases were subtyped. Three WGS clusters were identified: cluster 1 (n = 36), cluster 2 (n = 5) and cluster 3 (n = 3). Compared to controls, cluster 1 cases more frequently consumed raw smoked ham (odds ratio (OR) 10, 95% confidence interval (CI) 1.2–88) bought at supermarket chain X (OR 36, 95% CI 4–356; 9/10 consumed ham Y). All four cluster 2 cases interviewed had consumed quail eggs. Timely WGS was invaluable in distinguishing concurrent outbreaks of a rare Salmonella serotype.

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Copyright

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

Author for correspondence: J. Enkelmann E-mail: Enkelmannj@rki.de

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These authors contributed equally to this article and share first authorship.

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References

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1.Robert Koch-Institut. SURVSTAT@RKI 2.0. Available at https://survstat.rki.de.
2.Toboldt, A et al. (2014) Molecular epidemiology of Salmonella enterica serovar Kottbus isolated in Germany from humans, food and animals. Veterinary Microbiololgy 170, 97108.
3.Ryder, RW et al. (1977) Human milk contaminated with Salmonella kottbus. A cause of nosocomial illness in infants. Journal of the American Medical Association 238, 15331534.
4.Palmera-Suarez, R et al. (2007) Salmonella Kottbus outbreak in infants in Gran Canaria (Spain), caused by bottled water, August-November 2006. Eurosurveillance 12, pii3235.
5.Winthrop, KL et al. (2003) Alfalfa sprouts and Salmonella Kottbus infection: a multistate outbreak following inadequate seed disinfection with heat and chlorine. Journal of Food Protection 66, 1317.
6.Ribot, EM et al. (2006) Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathogens and Disease 3, 5967.
7.Maiden, MC et al. (1998) Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proceedings of the National Academy of Sciences of the USA 95, 31403145.
8.Simon, S et al. (2018) Evaluation of WGS based approaches for investigating a food-borne outbreak caused by Salmonella enterica serovar Derby in Germany. Food Microbiology 71, 4654.
9.European Centre for Disease Prevention and Control (ECDC). Epidemic Intelligence Information System (EPIS). Available at https://www.ecdc.europa.eu/en/publications-data/epidemic-intelligence-information-system-epis (Accessed 03.12.2019).
10.Mertens, PL et al. (1999) [An epidemic of Salmonella typhimurium associated with traditional salted, smoked, and dried ham]. Nederlands tijdschrift voor geneeskunde 143, 10461049.
11.Brandwagt, D et al. (2018) Outbreak of Salmonella Bovismorbificans associated with the consumption of uncooked ham products, the Netherlands, 2016 to 2017. Eurosurveillance 23, 1116.
12.Lyytikainen, O et al. (2000) Molecular epidemiology of an outbreak caused by Salmonella enterica serovar Newport in Finland and the United Kingdom. Epidemiology & Infection 124, 185192.
13.Huedo, P et al. (2016) A multischool outbreak due to Salmonella enterica serovar Napoli associated with elevated rates of hospitalizations and bacteremia, Milan, Italy, 2014. Foodborne Pathogens and Disease 13, 417422.
14.Jansen, A, Frank, C and Stark, K (2007) Pork and pork products as a source for human salmonellosis in Germany. Berliner und Muenchener Tieraerztliche Wochenschrift 120, 340346.
15.Schroeder, S et al. (2016) A prolonged outbreak of Salmonella Infantis associated with pork products in central Germany, April–October 2013. Epidemiology & Infection 144, 14291439.

Keywords

Disentangling outbreaks using whole-genome sequencing: concurrent multistate outbreaks of Salmonella Kottbus in Germany, 2017

  • J. Enkelmann (a1) (a2) (a3), A. von Laer (a1) (a2) (a3), S. Simon (a4), A. Fruth (a4), R. Lachmann (a1), K. Michaelis (a1), M. Borowiak (a5), S. Gillesberg Lassen (a6) and C. Frank (a1)...

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