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A comparison of the iodine and fluorescent antibody methods for staining trachoma inclusions in the conjunctiva*

Published online by Cambridge University Press:  15 May 2009

J. Sowa ,
L. H. Collier  and
Shiona Sowa
J. Sowa
Affiliation:
Medical Research Council Trachoma Unit, Fajara, The Gambia, and Lister Institute of Preventive Medicine, London, S. W. 1
L. H. Collier
Affiliation:
Medical Research Council Trachoma Unit, Fajara, The Gambia, and Lister Institute of Preventive Medicine, London, S. W. 1
Shiona Sowa
Affiliation:
Medical Research Council Trachoma Unit, Fajara, The Gambia, and Lister Institute of Preventive Medicine, London, S. W. 1
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In terms of the rate of positive diagnoses the indirect fluorescent antibody (FA) test was rather more effective than iodine for demonstrating trachoma (TRIC) inclusions in conjunctival scrapings, but the degree of advantage was not statistically significant. In duplicate scrapings stained at random by one or the other method, FA staining yielded the higher inclusion count significantly more often than did iodine. Some inclusions that failed to stain with FA were found on subsequent staining with Giemsa. A method is described for improving the post-FA Giemsa staining of conjunctival smears stored at subzero temperatures. Given adequate facilities, the FA stain is preferable to iodine for demonstrating TRIC inclusions in the conjunctiva; but the iodine method, properly used, holds advantages for field use.

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 4 , December 1971 , pp. 693 - 708
Copyright
Copyright © Cambridge University Press 1971

References

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