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Characterization of rotavirus electropherotypes excreted by symptomatic and asymptomatic infants

  • J. Fernández (a1), A. M. Sandino (a1), J. Pizarro (a1), L. F. Avendaño (a2), J. M. Pizarro (a1) and E. Spencer (a1)...

Summary

Human rotavirus isolates from 1100 stool samples were analyzed by polyacrylamide gel electrophoresis, and 48 different migration patterns were detected. Heterogeneity in the migration of segment 10 was observed in both long and short electropherotypes in which three long and two short patterns were identified. In spite of these variations all short and long electropherotypes were subgrouped by enzyme immunoassay as subgroups I and II respectively. Mixed infections were detected in 17% of cases and the subgrouping correlated with the corresponding electropherotypes. The same electropherotypes were present in severe, mild and asymptomatic cases and no electropherotype was particularly associated with greater virulence. Furthermore, the electropherotypes isolated from nosocomial asymptomatic cases were the same as those detected from those admitted with severe diarrheoa. It seems unlikely that electropherotyping can be used to identify more virulent strains of rotavirus.

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References

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1.Estes, MK, Palmer, EL, Obijeski, JF. Rotaviruses: A review. Curr Top Microbiol Immunol 1983; 105: 123–84.
2.Novo, E, Esparza, J. Composition and topography of structural polypeptide of bovine rotavirus. J Gen Virol 1981; 56: 325–35.
3.Sandino, AM, Jashes, M, Faundez, G, Spencer, E. Role of the inner protein capsid on in vitro rotavirus transcription. J Virol 1986; 60: 797802.
4.Herring, AJ, Inglis, NF, Ojeh, CK, Snodgrass, DR, Menzies, JD. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 1982; 16: 473–7.
5.Spencer, E, Avendaño, LF, Araya, M. Characteristic and analysis of electropherotypes of human rotavirus isolated in Chile. J Infect Dis 1983; 148: 41–8.
6.Avendaño, LF, Spencer, E, Calderon, A, Martinez, A. Infección por rotavirus en lactantes con diarrea aguda. Aspectos clínicos y epidemiológicos. Rev Med Chile 1983; 111: 240–6.
7.Laurenco, MH, Nicolas, JC, Cohen, J, Scherrer, R, Bricout, F. Study of human rotavirus genome by electrophoresis: attempt of classification among strains isolated in France. Annales de Virologie 1981; 132: 161–73.
8.Espejo, RT, Calderon, E, Gonzalez, N, Salomon, A, Martuscelli, A, Romero, P. Presence of two distinct types of rotavirus in infants and young children hospitalized with acute gastroenteritis in Mexico City. J Infect Dis 1979; 139: 474–7.
9.Kalica, AR, Greenberg, HB, Espejo, RT, Flores, J, Wyatt, RG, Kapikian, AZ, Chanock, RM. Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infect Immun 1981; 33: 958–61.
10.Kapikian, AZ, Chanock, RM. Rotaviruses. In Fields, BN, Knipe, DM, Chanock, RM, Melnick, JL, Roizman, B, Shope, RE, eds. Virology. New York: Raven Press. 1985; 863906.
11.Chanock, SJ, Wenske, EA, Fields, B. Human rotavirus and RNA genome. J Infect Dis 1983; 148: 49–5.
12.Flores, J, Hoshino, Y, Boeggeman, E, Purcell, R, Chanock, RM, Kapikian, AZ. Genetic relatedness among animal rotaviruses. Arch Virol 1986; 87: 273–85.
13.Rodger, SM, Bishop, RF, Birch, C, McLean, B, Holmes, IH. Molecular epidemiology of human rotavirus in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. J Clin Microbiol 1981; 13: 272–8.
14.Fukuhara, N, Yoshie, O, Kitaoka, S, Konno, T. Role of VP3 in human rotavirus internalization after target cell attachment via VP7. J Virol 1988; 62: 2209–18.
15.Greenberg, HB, Flores, J, Kalica, AR, Wyatt, RG, Jones, R. Gene coding assignments for growth restriction neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. J Gen Virol 1983; 64: 313–20.
16.Mackow, ER, Shaw, RD, Matsui, SM, Vo, PT, Dang, MN, Greenberg, HB. The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region. Proc Natl Acad Sci USA 1988; 85: 645–9.
17.Taniguchi, K, Urasawa, T, Morita, Y, Greenberg, HB, Urasawa, S. Direct serotyping of human rotavirus in stool by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3- and 4- specific monoclonal antibodies to VP7. J Infect Dis' 1987; 155: 11569–66.
18.Taniguchi, K. Hoshino, Y, Nishikawa, K et al. . Cross-reactive and serotype -specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol 1988; 62: 1870–4.
19.Kutsuzawa, T, Konno, T, Suzuki, H, Kapikian, AZ, Ebina, T, Ishida, N. Isolation of human rotavirus subgroups 1 and 2 in cell culture. J Clin Microbiol 1982; 16: 727–30.
20.Flores, J, Midthun, K, Hoshino, Y, Green, K. Gorziglia, M, Kapikian, AZ, Chanock, RM. Conservation of the fourth gene among rotaviruses recovered from asymptomatic newborn infants and its possible role in attenuation. J Virol 1986; 60: 972–9.
21.Flores, J, Sears, J, Green, KY et al. , Genetic stability of rotaviruses recovered from asymptomatic neonatal infections. J Virol 1988; 62: 4778–81.
22.Spencer, E, Araya, M, Sandino, AM, Pacheco, I, Brunser, O. Faecal excretion of rotavirus and other enteropathogens in newborns of the high and low socio-economic stratum in Santiago, Chile. Epidemiol Infect 1988; 101: 425–36.
23.Avendaño, LF, Barraza, P, Calderon, A, Matamala, I, Duarte, E, Spencer, E. Infección intrahospitalaria por rotavirus en lactantes. Revista Chilena de Infectiología 1986; 3: 8998.
24.Laemmli, UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 68O–5.
25.Spencer, E, Avendaño, LF, Garcia, BI. Analysis of human rotavirus mixed electrophoretypes. Infect Immun 1983; 39: 569–74.
26.Jashes, M, Sandino, AM, Faundez, G, Avendaño, LF, Spencer, E. In vitro transcription of human pararotavirus. J Virol 1986; 57: 183–90.
27.Cohen, J, Charpilienne, A, Chilmonczyk, S, Estes, MK. Nucleotide sequence of bovine rotavirus gene 1 and expression of the gene product in Baculovirus. Virol 1989; 171: 131140.
28.Nishikawa, K, Taniguchi, K, Torres, A et al. , Comparative analysis of the VP3 gene of divergent strains of the rotaviruses simian SA11 and bovine Nebraska calf diarrhea virus. J Virol 1988; 62: 4022–6.

Characterization of rotavirus electropherotypes excreted by symptomatic and asymptomatic infants

  • J. Fernández (a1), A. M. Sandino (a1), J. Pizarro (a1), L. F. Avendaño (a2), J. M. Pizarro (a1) and E. Spencer (a1)...

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