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Capsular polysaccharide and lipopolysaccharide O type analysis of Klebsiella pneumoniae isolates by genotype in China

  • Z. Y. Zhang (a1), R. Qin (a2), Y. H. Lu (a2), J. Shen (a2), S. Y. Zhang (a2), C. Y. Wang (a2), Y. Q. Yang (a2), F. P. Hu (a3) and P. He (a2)...

Abstract

Klebsiella pneumoniae is a common pathogen associated with nosocomial infections and is characterised serologically by capsular polysaccharide (K) and lipopolysaccharide O antigens. We surveyed a total of 348 non-duplicate K. pneumoniae clinical isolates collected over a 1-year period in a tertiary care hospital, and determined their O and K serotypes by sequencing of the wbb Y and wzi gene loci, respectively. Isolates were also screened for antimicrobial resistance and hypervirulent phenotypes; 94 (27.0%) were identified as carbapenem-resistant (CRKP) and 110 (31.6%) as hypervirulent (hvKP). isolates fell into 58 K, and six O types, with 92.0% and 94.2% typeability, respectively. The predominant K types were K14K64 (16.38%), K1 (14.66%), K2 (8.05%) and K57 (5.46%), while O1 (46%), O2a (27.9%) and O3 (11.8%) were the most common. CRKP and hvKP strains had different serotype distributions with O2a:K14K64 (41.0%) being the most frequent among CRKP, and O1:K1 (26.4%) and O1:K2 (17.3%) among hvKP strains. Serotyping by gene sequencing proved to be a useful tool to inform the clinical epidemiology of K. pneumoniae infections and provides valuable data relevant to vaccine design.

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Copyright

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

Authors for correspondence: P. He, E-mail: hpatsh@sjtu.edu.cn; F. P. Hu, E-mail: hufupin@fudan.edu.cn

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Contributed equally to this work.

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References

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1.Gomez-Simmonds, A and Uhlemann, AC (2017) Clinical implications of genomic adaptation and evolution of carbapenem-resistant Klebsiella pneumonia. Journal of Infectious Diseases 215(suppl. 1), S18S27.
2.Lee, CR et al. (2017) Antimicrobial resistance of hypervirulent Klebsiella pneumoniae: epidemiology, hypervirulence-associated determinants, and resistance mechanisms. Frontiers in Cellular and Infection Microbiology 7, 483.
3.Chen, L et al. (2014) Carbapenemase-producing Klebsiella pneumoniae: molecular and genetic decoding. Trends in Microbiology 22, 686696.
4.Fung, CP et al. (2000) A 5-year study of the seroepidemiology of Klebsiella pneumoniae: high prevalence of capsular serotype K1 in Taiwan and implication for vaccine efficacy. Journal of Infectious Diseases 181, 20752079.
5.Podschun, R and Ullmann, U (1998) Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clinical Microbiology Reviews 11, 589663.
6.Brisse, S et al. (2013) Wzi gene sequencing, a rapid method for determination of capsular type for Klebsiella strains. Journal of Clinical Microbiology 51, 40734078.
7.Fang, CT et al. (2016) Rapid and accurate determination of lipopolysaccharide O-antigen types in Klebsiella pneumoniae with a novel PCR-based O-genotyping method. Journal of Clinical Microbiology 54, 666675.
8.WHO's first global report on antibiotic resistance reveals serious, worldwide threat to public health. 2014; Available at: https://www.who.int/news-room/detail/30-04-2014-who-s-first-global-report-on-antibiotic-resistance-reveals-serious-worldwide-threat-to-public-health.
9.Wu, D, Cai, J and Liu, J (2011) Risk factors for the acquisition of nosocomial infection with carbapenem-resistant Klebsiella pneumoniae. Southern Medical Journal 104, 106110.
10.Orskov, I and Orskov, F. Serotyping of Klebsiella. In Bergan (ed.), Methods in Microbiology, vol 14, London: Academic Press, pp. 143164.
11.Simoons-Smit, AM et al. (1984) Virulence of Klebsiella strains in experimentally induced skin lesions in the mouse. Journal of Medical Microbiology 17, 6777.
12.Simoons-Smit, AM et al. (1985) Biochemical and serological investigations on clinical isolates of Klebsiella. Journal of Hygiene (London) 95, 265276.
13.Jenney, AW et al. (2006) Seroepidemiology of Klebsiella pneumoniae in an Australian tertiary hospital and its implications for vaccine development. Journal of Clinical Microbiology 44, 102107.
14.Turton, JF et al. (2007) Genetically similar isolates of Klebsiella pneumoniae serotype K1 causing liver abscesses in three continents. Journal of Medical Microbiology 56, 593597.
15.Pan, YJ et al. (2015) Identification of capsular types in carbapenem-resistant Klebsiella pneumoniae strains by wzc sequencing and implications for capsule depolymerase treatment. Antimicrobial Agents and Chemotherapy 59, 10381047.
16.Liu, Y et al. (2017) Capsular polysaccharide types and virulence-related traits of epidemic KPC-producing Klebsiella pneumoniae isolates in a Chinese University Hospital. Microbial Drug Resistance 23, 901907.
17.Russso, TA and Marr, CM (2019) Hypervirulent Klebsiella pneumoniae. Clinical Microbiology Reviews 32, e0000119.

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Capsular polysaccharide and lipopolysaccharide O type analysis of Klebsiella pneumoniae isolates by genotype in China

  • Z. Y. Zhang (a1), R. Qin (a2), Y. H. Lu (a2), J. Shen (a2), S. Y. Zhang (a2), C. Y. Wang (a2), Y. Q. Yang (a2), F. P. Hu (a3) and P. He (a2)...

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