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Blastocystis: unravelling potential risk factors and clinical significance of a common but neglected parasite

  • C. R. STENSVOLD (a1), H. C. LEWIS (a2), A. M. HAMMERUM (a3), L. J. PORSBO (a4), S. S. NIELSEN (a1), K. E. P. OLSEN (a1), M. C. ARENDRUP (a1), H. V. NIELSEN (a1) and K. MØLBAK (a2)...

Summary

Two independent studies were conducted to describe symptoms and potential risk factors associated with Blastocystis infection. Isolates were subtyped by molecular analysis. In the NORMAT study (126 individuals randomly sampled from the general population) 24 (19%) were positive for Blastocystis. Blastocystis was associated with irritable bowel syndrome (P=0·04), contact with pigs (P<0·01) and poultry (P=0·03). In the Follow-up (FU) study (follow-up of 92 Blastocystis-positive patients), reports on bloating were associated with subtype (ST) 2 (P<0·01), and blood in stool to mixed subtype infection (P=0·06). ST1 was more common in FU individuals (32%) than in NORMAT individuals (8%), whereas single subtype infections due to ST3 or ST4 were seen in 63% of the NORMAT cases and 28% of the FU cases. Only FU individuals hosted ST7, and ST6/7 infections due to ST7 or ST9 were characterized by multiple intestinal symptoms. The data indicate subtype-dependent differences in the clinical significance of Blastocystis.

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Copyright

Corresponding author

*Author for correspondence: Dr C. R. Stensvold, Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. (Email: RUN@ssi.dk)

References

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1. Stenzel, DJ, Boreham, PFL. Blastocystis hominis revisited. Clinical Microbiology Reviews 2006; 9: 563584.
2. Stensvold, CR, et al. Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype. International Journal for Parasitology (in press).
3. Noël, C, et al. Molecular phylogenies of Blastocystis isolates from different hosts: Implications for genetic diversity, identification of species and zoonosis. Journal of Clinical Microbiology 2005; 43: 348355.
4. Scicluna, SM, Tawari, B, Clark, CG. DNA barcoding of Blastocystis. Protist 2006; 157: 7785.
5. Parkar, U, et al. Direct characterization of Blastocystis from feces by PCR and evidence of zoonotic potential. Parasitology 2007; 134: 359367.
6. Böhm-Gloning, B, Knobloch, J, Walderich, B. Five sub-groups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR-amplified 16S-like rDNA. Tropical Medicine and International Health 1997; 2: 771778.
7. Yoshikawa, H, et al. Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities. Journal of Clinical Microbiology 2000; 38: 13241330.
8. Kaneda, Y, et al. Ribodemes of Blastocystis hominis isolated in Japan. American Journal of Tropical Medicine and Hygiene 2001; 65: 393396.
9. Tan, TC, et al. PCR fingerprinting of Blastocystis isolated from symptomatic and asymptomatic hosts. Parasitology Research 2006; 99: 459465.
10. Yan, Y, et al. Genetic variability of Blastocystis hominis isolates in China. Parasitology Research 2006; 99: 597601.
11. Özyurt, M, et al. Molecular epidemiology of Blastocystis in Turkey. Parasitology International 2008; 57: 300306.
12. Tan, KS. Blastocystis in humans and animals: new insights using modern methodologies. Veterinary Parasitology 2004; 126: 121144.
13. Stensvold, CR, et al. Terminology for Blastocystis subtypes – a consensus. Trends in Parasitology 2007; 23: 9396.
14. Clark, CG. Extensive genetic diversity in Blastocystis hominis. Molecular and Biochemical Parasitology 1997; 87: 7983.
15. Arisue, N, Hashimoto, T, Yoshikawa, H. Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates. Parasitology 2003; 126: 19.
16. Stensvold, CR, et al. Symptomatic Blastocystis infection successfully treated with trimethoprim/sulfamethoxazole. Annals of Tropical Medicine and Parasitology 2008; 102: 271274.
17. Windsor, JJ, et al. Incidence of Blastocystis hominis in faecal samples submitted for routine microbiological analysis. British Journal of Biomedical Sciences 2002; 59: 154157.
18. Stensvold, CR, et al. The prevalence of Dientamoeba fragilis in patients with suspected enteroparasitic disease in a metropolitan area in Denmark. Clinical Microbiology and Infection 2007; 13: 839842.
19. Stensvold, CR, et al. Detecting Blastocystis by parasitological and DNA-based methods: A comparative study. Diagnostic Microbiology and Infectious Disease 2007; 59: 303307.
20. Taamasri, P, et al. Prevalence of Blastocystis hominis carriage in Thai army personnel based in Chonburi, Thailand. Military Medicine 2002; 167: 643646.
21. Li, L-H, et al. Molecular epidemiology of Blastocystis hominis in a rural village in Yunnan province, China. Parasitology International 2007; 54: 281286.
22. Persson, S, et al. A method for fast and simple detection of major diarrheagenic Escherichia coli in the routine diagnostic laboratory. Clinical Microbiology and Infection 2007; 13: 516524.
23. Stensvold, R, et al. Detection of Blastocystis hominis in unpreserved stool specimens using polymerase chain reaction. Journal of Parasitology 2006; 92: 10811087.
24. Stensvold, CR, et al. Blastocystis: subtyping isolates using Pyrosequencing™ technology. Experimental Parasitology 2007; 116: 111119.
25. Stensvold, CR, et al. Prevalence of Dientamoeba fragilis genotypes in different Danish populations as assessed by PCR and SNP genotyping. Tropical Medicine and International Health 2007; 12 (Suppl.): 182.
26. Wong, KH, et al. Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Parasitology Research 2008; 102: 663670.
27. Hussain, R, et al. Significantly increased IgG2 subclass antibody levels to Blastocystis hominis in patients with irritable bowel syndrome. American Journal of Tropical Medicine and Hygiene 1997; 56: 301306.
28. Giacometti, A, et al. Irritable bowel syndrome in patients with Blastocystis hominis infections. European Journal of Clinical Microbiology and Infectious Diseases 1999; 18: 436439.
29. Tungtrongchitr, A, et al. Blastocystis hominis infection in irritable bowel syndrome patients. South East Asian Journal of Tropical Medicine and Public Health 2004; 35: 705710.
30. Yakoob, J, et al. In vitro susceptibility of Blastocystis hominis isolated from patients with irritable bowel syndrome. British Journal of Biomedical Sciences 2004; 61: 7577.
31. Lee, MJ. Pathogenicity of Blastocystis hominis. Journal of Clinical Microbiology 1991; 29: 2089.
32. Lakhanpal, S, Cohen, SB, Fleischmann, RM. Reactive arthritis from Blastocystis hominis. Arthritis and Rheumatism 1991; 34: 251253.
33. Krüger, K, Kamilli, I, Schattenkirchner, M. Blastocystis hominis as a rare arthritogenic pathogen. A case report. Zeitschrift für Rheumatologie 1994; 53: 8385.
34. Abe, N, Wu, Z, Yoshikawa, H. Molecular characterization of Blastocystis isolates from birds by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene. Parasitology Research 2003; 89: 393396.
35. Yoshikawa, H, et al. Molecular comparative studies among Blastocystis isolates obtained from humans and animals. Journal of Parasitology 2003; 89: 585594.
36. Abe, N. Molecular and phylogenetic analysis of Blastocystis isolates from various hosts. Veterinary Parasitology 2004; 120: 235242.
37. Yan, Y, et al. Blastocystis sp. subtype 5: a possibly zoonotic genotype. Parasitology Research 2007; 101: 15271532.
38. Crotti, D, et al. Dientamoeba fragilis in swine population: a preliminary investigation. Veterinary Parasitology 2007; 145: 349351.
39. Stark, D, et al. Gorillas are a host for Dientamoeba fragilis: an update on the life cycle and host distribution. Veterinary Parasitology 2008; 151: 2126.
40. Nigro, L, et al. A placebo-controlled treatment trial of Blastocystis hominis infection with metronidazole. Journal of Travel Medicine 2003; 10: 128130.

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Blastocystis: unravelling potential risk factors and clinical significance of a common but neglected parasite

  • C. R. STENSVOLD (a1), H. C. LEWIS (a2), A. M. HAMMERUM (a3), L. J. PORSBO (a4), S. S. NIELSEN (a1), K. E. P. OLSEN (a1), M. C. ARENDRUP (a1), H. V. NIELSEN (a1) and K. MØLBAK (a2)...

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