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Audit of scope and culture techniques applied to samples for the diagnosis of Mycobacterium bovis by hospital laboratories in England and Wales

Published online by Cambridge University Press:  29 April 2003

F. DROBNIEWSKI ,
M. STRUTT ,
G. SMITH ,
J. MAGEE  and
P. FLANAGAN
F. DROBNIEWSKI
Affiliation:
PHLS Mycobacterium Reference Unit and Regional Centre for Mycobacteriology, South London Public Health Laboratory and Department of Infection Guy's, King's & St. Thomas' School of Medicine, Kings College Hospital (Dulwich), East Dulwich Grove, London SE22 8QF, UK
M. STRUTT
Affiliation:
PHLS Mycobacterium Reference Unit and Regional Centre for Mycobacteriology, South London Public Health Laboratory and Department of Infection Guy's, King's & St. Thomas' School of Medicine, Kings College Hospital (Dulwich), East Dulwich Grove, London SE22 8QF, UK
G. SMITH
Affiliation:
Regional Centre for Mycobacteriology, Birmingham Heartlands Hospital, UK
J. MAGEE
Affiliation:
Regional Centre for Mycobacteriology, Newcastle General Hospital, UK
P. FLANAGAN
Affiliation:
Regional Centre for Mycobacteriology, University Hospital of Wales, Cardiff, UK
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Abstract

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This audit examines the ability of English and Welsh laboratories to diagnose Mycobacterium bovis infection. All 164 clinical laboratories submitting samples to the PHLS Mycobacterium Reference Unit and Regional Centres for Mycobacteriology were surveyed. Twenty per cent of responding centres did not use a pyruvate-containing medium or incubate for the minimum recommended period of 8 weeks. This study demonstrates the potential for the underdiagnosis of M. bovis infection in England and Wales. Possible reasons for underdiagnosis are discussed together with strategies to optimize recovery of M. bovis.

Type
Research Article
Information
Epidemiology & Infection , Volume 130 , Issue 2 , April 2003 , pp. 235 - 237
Copyright
© 2003 Cambridge University Press