Skip to main content Accessibility help
×
Home

Apramycin-resistant Escherichia coli isolated from pigs and a stockman

  • J. E. B. Hunter (a1) (a2), M. Bennett (a1), C. A. Hart (a2), J. C. Shelley (a1) and J. R. Walton (a1)...

Summary

Escherichia coli serotype O147:K89:K88a,c was found to be associated with outbreaks of diarrhoea in preweaner pigs of up to 4 weeks of age on a pig unit. Resistance to apramycin, gentamicin, netilmicin, tobramycin and other antibiotics was associated with conjugative plasmids of approximately 62 kb. The presence of a gene which encoded for the aminoglycoside acetyltransferase enzyme AAC(3)IV was confirmed by DNA hybridization.

Samples collected during the following 12 months revealed widespread dissemination of these resistance plasmids in non-serotypable, non-haemolytic E. coli throughout the farm. Apramycin-resistant E. coli were also isolated from a stockman and it appeared from plasmid profile analysis and antibiotic sensitivity testing that the human isolates carried the same plasmid as that carried by the porcine E. coli. Klebsiella pneumoniae, with a slightly smaller conjugative plasmid and similar resistance pattern, was isolated from the stockman's wife.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Apramycin-resistant Escherichia coli isolated from pigs and a stockman
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Apramycin-resistant Escherichia coli isolated from pigs and a stockman
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Apramycin-resistant Escherichia coli isolated from pigs and a stockman
      Available formats
      ×

Copyright

References

Hide All
1.Sojka, WJ. Lloyd, MK. Sweeny, EJ. Escherichia coli serotypes associated with certain pig diseases. Res Vet Sci 1960; 1: 1727.
2.So, M. Crandell, IF. Crosa, JH. Falkow, S. Extrachromosomal determinants which contribute to bacterial pathogenicity. In: Schlessinger, D. ed. Microbiology. Washington, DC: American Society for Microbiology, 1974; 1626.
3.Hunter, JEB. Some studies on multi-resistant E. coli and the use of antibiotics in the treatment of diarrhoea in pigs. Pig Vet Journal, Pig Vet Soc 1993; 31: 143–51.
4.Chaslus-Dancla, E, Martel, J. Carlier, C. Lafont, J, Courvalin, P. Emergence of aminoglycoside 3-N-acetyl transferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France. Antimicrob Agents Chemother 1986; 29: 239–43.
5.Wray, C. Hedges, RW. Shannon, KP, Bradley, DE. Apramycin and gentamicin resistance in Escherichia coli and salmonellas isolated from animals. J Hyg 1986; 97: 445–6.
6.Hunter, JEB, Shelley, JC, Walton, JR, Hart, CA, Bennett, M. Apramycin resistance plasmids in Escherichia coli: possible transfer to Salmonella typhimurium in calves. Epidemiol Infect 1992; 108: 271–8.
7.Threlfall, EJ, Rowe, B. Ferguson, JL, Ward, LR. Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg 1986; 97: 419–26.
8.Chaslus-Dancla, E, Glupczynski, Y, Gerbaud, G, Lagorce, M, Lafont, JP, Courvalin, P. Detection of apramycin resistant Enterobacteriaceae in hospital isolates. FEMS Microbiol Lett 1989; 61: 261–6.
9.Hunter, JEB. Hart, CA, Shelley, JC. Walton, JR, Bennett, M. Human isolates of apramycin-resistant Escherichia coli which contain the genes for the AAC(3)IV enzyme. Epidemiol Infect 1993; 110: 253–9.
10.Stokes, EJ, Waterworth, PN. Antibiotic sensitivity tests by diffusion methods. Assoc Clin Pathol Broadsheets 1973; 55: 112.
11.Kado, CI. Liu, ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145: 1365–73.
12.Brau, B, Pilz, U, Piepersberg, W. Genes for gentamicin-(3)-N-acetyltransferases III and IV. 1. Nucleotide sequence of the AAC(3)-IV gene and possible involvement of an 1S140 element in its expression. Mol Genet 1984; 193: 179–87.
13.Sambrook, J, Fritsch, EF, Maniatis, T. In: Molecular cloning: a laboratory manual, vol 2. 2nd ed.Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 1989: 9.529.55.
14.Ose, EE, Ryden, R. Muenster, OA. Apramycin, a new aminocyclitol antibiotic. 1. In vitro evaluation. Proc Fourth Int Pig Vet Soc Congress, Ames, Iowa. 1976.
15.Duff, JP. Hunt, BW. Lambs die from porcine E. coli. Vet Rec 1989: 125: 404.
16.Jorgensen, ST. Sorensen, VW. Spread of an R plasmid among antigen types of Escherichia coli pathogenic for piglets. Plasmid 1979; 2: 290–2.
17.Mee, BJ, Nikoletti, SM. Plasmids encoding trimethoprim resistance in bacterial isolates from man and pigs. J Appl Bact 1983; 54: 225–35.
18.Hunter, JEB. Apramycin-resistant Escherichia coli in animals and man [PhD thesis]. University of Liverpool. 1992: 68154.
19.Johnson, AP, Burns, L, Woodford, N et al. , Gentamicin resistance in clinical isolates of Escherichia coli encoded by genes of veterinary origin. J Med Micro. In press.
20.Langlois, BE. Cromwell, GL, Stahly, TS. Dawson, KA. Hays, VW. Antibiotic resistance of faecal coliforms after long-term withdrawal of therapeutic and subtherapeutic use in a swine herd. Appl Envir Micro 1983; 46: 1433–4.
21.Gellin, G, Langlois, BE. Dawson, KA, Aaron, DK. Antibiotic resistance of Gram-negative enteric bacteria from pigs in three herds with different histories of antibiotic exposure. Appl Envir Micro 1989; 55: 2287–92.

Apramycin-resistant Escherichia coli isolated from pigs and a stockman

  • J. E. B. Hunter (a1) (a2), M. Bennett (a1), C. A. Hart (a2), J. C. Shelley (a1) and J. R. Walton (a1)...

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed