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Antibiotic residues in meat in the United Kingdom; an assessment of specific tests to detect and identify antibiotic residues

Published online by Cambridge University Press:  19 October 2009

R. Smither
Affiliation:
Laboratory of the Government Chemist, Department of Industry, Cornwall House, Stamford Street, London SE1 9NQ
A. F. Lott
Affiliation:
Laboratory of the Government Chemist, Department of Industry, Cornwall House, Stamford Street, London SE1 9NQ
R. W. Dalziel
Affiliation:
Veterinary Investigation Centre, Government Buildings, Coley Park, Reading RG1 6DT
D. C. Ostler
Affiliation:
Veterinary Investigation Centre, Government Buildings, Coley Park, Reading RG1 6DT
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Summary

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Investigations were conducted between 1977 and 1979 to assess the performance of microbiological tests for detecting and identifying residues of therapeutic-type antibacterial substances in meat and offal. Of the 5442 home-produced meat samples examined, 34 (0·63%) showed inhibitory activity in the screening test, which used Bacillus subtilis BGA and Micrococcus luteus as indicator organisms. Identification by electrophoretic and thin-layer chromatography/bio-autography techniques confirmed that only two of the 34 screen failures were due to true antibacterial residues: a pig sample contained a trace of penicillin and a horse sample contained a trace of an incompletely identified substance resembling a tetracycline. Twelve of the other 32 failures in the screen test were due to naturally produced inhibition and were, thus, falsely positive, whilst the remainder were shown to be negative. All of the 85 (8·7%) screen test failures from the 972 imported meat and offal samples tested were falsely positive. Additional samples from certain animals known to have been given antibiotic treatment were tested concurrently to give a more searching indication of screen and identification test efficacy.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Anderson, E. S. (1975). Tho problem and implications of chlornmphenicol resistance in the typhoid bacillus. Journal of Hygiene 74, 289.CrossRefGoogle Scholar
Anderson, E. S. & Smith, H. R. (1972). Chloramphenicol resistance in tho typhoid bacillus. British Medical Journal iii, 329.CrossRefGoogle Scholar
Anon. (1974 a). Antibiotic-resistant organisms and food. British Food Journal 76, 100.Google Scholar
Anon. (1974 b). Verordnung zur Änderung der Ausführungsbestimmungen A über die Untcrsuchung und gesundhcitspolizeiliche Behandlung der Schlanchttiere und des Fleisches bei Schlachtungen im Inland. Bundesgesetzblatt Teil 1, 18.Google Scholar
Baggot, J. D. (1977). Principles of Drug Disposition in Domestic Animals; the Basis of Veterinary Clinical Pharmacology. Philadelphia: W. B. Saundere.Google Scholar
European Communities Council Directive 72/462/EEC. (1972). L302, 28.Google Scholar
Nouws, J. F. M., van Schothorst, M. & Ziv, G. (1979). A critical evaluation of several microbiological test methods for residues of antimicrobial drugs in ruminants. Archiv für Lebensmittelhygiene 30, 4.Google Scholar
Report (1909). Joint Committee on the use of antibiotics in animal husbandry and veterinary medicine. Cmnd. 4190. London: HMSO.Google Scholar
Smither, R. (1978). Bactorial inhibitors formed during the adventitious growth of micro-organisms in chicken liver and pig kidney. Journal of Applied Bacteriology 45, 267.CrossRefGoogle Scholar
Smither, R. & Vaughan, D. R. (1978). An improved electrophoretic method for identifying antibiotics with special reference to animal tissues and animal feeding stuffs. Journal of Applied Bacteriology 44, 421.CrossRefGoogle ScholarPubMed
Threlfall, E. J., Ward, L. R. & Rowe, B. (1978 a). Spread of multiresistant strains of Salmonella typhimurium phage types 204 and 193 in Britain. British Medical Journal ii, 997.CrossRefGoogle Scholar
Threlfall, E. J., Ward, L. R. & Rowe, B. (1978 b). Epidemic spread of a chloramphenicol-resistant strain of Salmonella typhimurium phago type 204 in bovine animals in Britain. Veterinary Record 103, 438.CrossRefGoogle Scholar