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An evaluation of Rappaport's magnesium chloride/malachite green medium in the routine examination of faeces

  • W. L. Hooper (a1) and H. R. Jenkins (a1)

Extract

Rappaport's magnesium chloride/malachite green medium was employed in the cultural examination of 2476 faecal specimens in parallel with selenite F broth enrichment and direct plate culture on deoxycholate citrate agar. The use of the medium increased the salmonella isolation rate by 26% over the number of isolations from the other two methods, but proved of no additional value in the isolation of shigella organisms. The addition of Rappaport medium is recommended in routine faecal examination.

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Copyright

References

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Armstrong, E. C. (1954). The relative efficacy of culture media in the isolation of Shigella sonnei. Mon. Bull. Minist. Kith, 13, 70.
Collard, P. & Unwin, M. (1958). A trial of Rappaport's medium. J. din. Path. 11, 426.
Hynes, M. (1942). Isolation of intestinal pathogens by selective media. J. Path. Bad. 54, 193.
Iveson, J. B., Kovacs, N. & Laurie, W. (1964). An improved method of isolating sal-monellae from contaminated desiccated coconut. J. din. Path. 17, 75.
Knox, R. (1949). A screening plate for the rapid identification of faecal organisms. J. Path. Bad. 61, 343.
Leifson, E. (1936). New selenite enrichment media for the isolation of typhoid and paratyphoid (salmonella) bacilli. Am. J. Hyg. 24, 423.
Rappaport, F., Konforti, N. & Navon, B. (1956). A new enrichment medium for certain salmonellae. J. din. Path. 9, 261.
Sen, R. (1964). An assessment of the relative efficiency of several media in the isolation of shigellae and salmonellae from faeces. Indian J. Path. Bad. 7, 93.
Thomson, S. (1954). The number of bacilli harboured by enteric carriers. J. Hyg., Camb., 52, 67.
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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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