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The adsorption of endotoxin molecule in a microporous polyethylene hollow fibre membrane

  • Yosuke Sawada (a1), Reiko Fujii (a1), Ikuo Igami (a1), Atsushi Kawai (a1), Teruo Kamiki (a2) and Makoto Niwa (a3)...

Summary

The microporous polyethylene hollow fibre membrane is capable of adsorbing small-sized lipopolysaccharides (LPS) prepared by sonication dispersion, column chromatography on Sephadex G-75 and filtration through a filter membrane with a nominal pore size of 0·025 μg. Small-sized LPS had a one-thousandth of endotoxin activity as compared to intact LPS, when determined by the Synthetic Chromogenic Substrate method of LAL with a specific endotoxin activity of 73·7 ng/μg LPS. Fluorescent microscopy of fluorescein conjugated LPS on a microporous polyethylene hollow fibre showed that fiuorescein-LPS was adsorbed through the entire depth of the membrane texture. Accordingly the adsorption capacity of the filter for small-sized LPS was determined as 1·65 mg LPS/3·68 m2 surface/116 mg fibre/module.

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References

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The adsorption of endotoxin molecule in a microporous polyethylene hollow fibre membrane

  • Yosuke Sawada (a1), Reiko Fujii (a1), Ikuo Igami (a1), Atsushi Kawai (a1), Teruo Kamiki (a2) and Makoto Niwa (a3)...

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