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Effects of high-intensity training on monocarboxylate transporters in Thoroughbred horses

Published online by Cambridge University Press:  04 March 2010

Yu Kitaoka ,
Yuki Wakasugi ,
Daisuke Hoshino ,
Kazutaka Mukai ,
Atsushi Hiraga  and
Hideo Hatta
Yu Kitaoka
Affiliation:
Department of Life Sciences (Sports Science), The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo153-8902, Japan
Yuki Wakasugi
Affiliation:
Department of Life Sciences (Sports Science), The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo153-8902, Japan
Daisuke Hoshino
Affiliation:
Department of Life Sciences (Sports Science), The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo153-8902, Japan
Kazutaka Mukai
Affiliation:
Equine Research Institute, Japan Racing Association, 321-4 Tokami-cho, Utsunomiya City, Tochigi Prefecture320-0856, Japan
Atsushi Hiraga
Affiliation:
Equine Research Institute, Japan Racing Association, 321-4 Tokami-cho, Utsunomiya City, Tochigi Prefecture320-0856, Japan
Hideo Hatta*
Affiliation:
Department of Life Sciences (Sports Science), The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo153-8902, Japan
*
*Corresponding author: hatta@idaten.c.u-tokyo.ac.jp
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Abstract

The aim of this study was to investigate the effect of high-intensity training on lactate transporters (monocarboxylate transporters (MCTs)) in Thoroughbred horses. Five Thoroughbreds (three males and two geldings; 3–10 years old; 478 ± 13 kg) ran at 100% maximal oxygen uptake (VO2max) for 1 min (3 days per week, 3 weeks) or 2 min (2 days per week, 4 weeks) on a treadmill for 7 weeks. VO2max and ability to sustain an incremental all-out test of maximal running were measured, and muscle samples were obtained from the gluteus medius muscle at rest before and after the training. The performance in the all-out test and VO2max were increased after the training. The protein content of MCT1 and MCT4 in the gluteus medius muscles was not significantly altered. However, MCT1 showed a relationship with the duration of maximal exercise after the training. The changes in MCT4 showed a relationship with the increase in exercise duration by the training. These results suggest that in Thoroughbreds, although the overall changes in MCT protein after endurance training are small, MCT1 and MCT4 can play important roles in the capacity to sustain maximal exercise.

Keywords

Thoroughbreds; high intensity trainingexercise capacitylactateMCT1MCT4
Type
Research Paper
Information
Comparative Exercise Physiology , Volume 6 , Issue 4 , November 2009 , pp. 171 - 175
Copyright
Copyright © Cambridge University Press 2010

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References

1 Snow, DH, Harris, RC and Gash, SP (1985). Metabolic response of equine muscle to intermittent maximal exercise. Journal of Applied Physiology 58: 1689–1697.CrossRefGoogle ScholarPubMed
2 Weber, JM, Parkhouse, WS, Dobson, GP, Harman, JC, Snow, DH and Hochachka, PW (1987). Lactate kinetics in exercising Thoroughbred horses: regulation of turnover rate in plasma. American Journal of Physiology 253: R896–R903.Google ScholarPubMed
3 Brooks, GA (2000). Intra- and extra-cellular lactate shuttles. Medicine and Science in Sports and Exercise 32: 790–799.CrossRefGoogle ScholarPubMed
4 Juel, C and Halestrap, AP (1999). Lactate transport in skeletal muscle – role and regulation of the monocarboxylate transporter. Journal of Physiology 517: 633–642.CrossRefGoogle ScholarPubMed
5 Bonen, A (2000). Lactate transporters (MCT proteins) in heart and skeletal muscles. Medicine and Science in Sports and Exercise 32: 778–789.CrossRefGoogle ScholarPubMed
6 Bonen, A, Heynen, M and Hatta, H (2006). Distribution of monocarboxylate transporters MCT1–MCT8 in rat tissues and human skeletal muscle. Applied Physiology, Nutrition, and Metabolism 31: 31–39.CrossRefGoogle ScholarPubMed
7 Wilson, MC, Jackson, VN, Heddle, C, Price, NT, Pilegaard, H, Juel, C et al. (1998). Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3. The Journal of Biological Chemistry 273: 15920–15926.CrossRefGoogle ScholarPubMed
8 Manning Fox, JE, Meredith, D and Halestrap, AP (2000). Characterization of human monocarboxylate transporter 4 substantiates its role in lactic acid efflux from skeletal muscle. Journal of Physiology 529: 285–293.Google ScholarPubMed
9 Yoshida, Y, Hatta, H, Kato, M, Enoki, T, Kato, H and Bonen, A (2004). Relationship between skeletal muscle MCT1 and accumulated exercise during voluntary wheel running. Journal of Applied Physiology 97: 527–534.CrossRefGoogle ScholarPubMed
10 Pilegaard, H, Domino, K, Noland, T, Juel, C, Hellsten, Y, Halestrap, AP et al. (1999). Effect of high-intensity exercise training on lactate/H+ transport capacity in human skeletal muscle. American Journal of Physiology: Endocrinology and Metabolism 276: 255–261.Google ScholarPubMed
11 Koho, NM, Vaihkonen, LK and Poso, AR (2002). Lactate transport in red blood cells by monocarboxylate transporters. Equine Veterinary Journal 34: 555–559.CrossRefGoogle Scholar
12 Koho, NM, Hyyppa, S and Poso, AR (2006). Monocarboxylate transporters (MCT) as lactate carriers in equine muscle and red blood cells. Equine Veterinary Journal 36: 354–358.CrossRefGoogle Scholar
13 Baker, SK, McCullagh, KJA and Bonen, A (1998). Training intensity-dependent and tissue-specific increases in lactate uptake and MCT-1 in heart and muscle. Journal of Applied Physiology 84: 987–994.CrossRefGoogle ScholarPubMed
14 Yamano, S, Eto, D, Hiraga, A and Miyata, H (2006). Recruitment pattern of muscle fibre type during high intensity exercise (60–100% VO2max) in Thoroughbred horses. Research in Veterinary Science 80: 109–115.CrossRefGoogle ScholarPubMed
15 Mukai, K, Ohmura, H, Hiraga, A, Eto, D, Takahashi, T, Asai, Y et al. (2006). Effect of detraining on cardiorespiratory variables in young thoroughbred horses. Equine Veterinary Journal Supplement 36: 210–213.CrossRefGoogle Scholar
16 Eto, D, Yamano, S, Mukai, K, Sugiura, T, Nasu, T, Tokuriki, M et al. (2004). Effect of high intensity training on anaerobic capacity of middle gluteal muscle in Thoroughbred horses. Research in Veterinary Science 76: 139–144.CrossRefGoogle ScholarPubMed
17 Fishbein, WN, Merezhinskaya, N and Foellmer, JW (2002). Relative distribution of three major lactate transporters in frozen human tissues and their localization in unfixed skeletal muscle. Muscle & Nerve 26: 101–112.CrossRefGoogle ScholarPubMed
18 Reeben, M, Koho, NM, Raekallio, M, Hyyppa, S and Poso, AR (2006). MCT1 and CD147 gene polymorphisms in standardbred horses. Equine Veterinary Journal Supplement 36: 322–325.CrossRefGoogle Scholar
19 Yoshida, Y, Holloway, GP, Ljubicic, V, Hatta, H, Spriet, LL, Hood, DA et al. (2007). Negligible direct lactate oxidation in subsarcolemmal and intermyofibrillar mitochondria obtained from red and white rat skeletal muscle. Journal of Physiology 582: 1317–1335.CrossRefGoogle ScholarPubMed
20 Enoki, T, Yoshida, Y, Hatta, H and Bonen, A (2003). Exercise training alleviates MCT1 and MCT4 reductions in heart and skeletal muscles of STZ-induced diabetic rats. Journal of Applied Physiology 94: 2433–2438.CrossRefGoogle ScholarPubMed
21 Rivero, JL, Ruz, A, Marti-Korff, S, Estepa, JC, Aguilera-Tejero, E, Werkman, J et al. (2007). Effects of intensity and duration of exercise on muscular responses to training of thoroughbred race horses. Journal of Applied Physiology 102: 1871–1882.CrossRefGoogle Scholar
22 Langsetmo, I and Poole, DC (1999). VO2 recovery kinetics in the horse following moderate, heavy, and severe exercise. Journal of Applied Physiology 86: 1170–1177.CrossRefGoogle ScholarPubMed
23 Baldwin, KM, Hooker, AM and Herrick, RE (1978). Lactate oxidative capacity in different types of muscle. Biochemical and Biophysical Research Communications 83: 151–157.CrossRefGoogle ScholarPubMed
24 Poso, AR, Essen-Gustavsson, B and Persson, SG (1993). Metabolic response to standardised exercise test in standardbred trotters with red cell hypervolaemia. Equine Veterinary Journal 25: 527–531.CrossRefGoogle ScholarPubMed
25 Thomas, C, Perrey, S, Lambert, K, Hugon, G, Mornet, D and Mercier, J (2005). Monocarboxylate transporters, blood lactate removal after supramaximal exercise, and fatigue indexes in humans. Journal of Applied Physiology 98: 804–809.CrossRefGoogle ScholarPubMed
26 Dimmer, KS, Friedrich, B, Lang, F, Deitmer, JW and Bröer, S (2000). The low-affinity monocarboxylate transporter MCT4 is adapted to the export of lactate in highly glycolytic cells. The Biochemical Journal 350: 219–227.CrossRefGoogle Scholar