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Variation analysis of the ORF5 gene of Porcine reproductive and respiratory syndrome virus

Published online by Cambridge University Press:  12 February 2007

Gao Zhi-Qiang ,
Guo Xin ,
Cha Zhen-Lin ,
Chen Yan-Hong  and
Yang Han-Chun
Gao Zhi-Qiang
Affiliation:
Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
Guo Xin
Affiliation:
Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
Cha Zhen-Lin
Affiliation:
Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
Chen Yan-Hong
Affiliation:
Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
Yang Han-Chun*
Affiliation:
Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
*
*Corresponding author: E-mail: yanghanchun1@cau.edu.cn
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Abstract

Three Porcine reproductive and respiratory syndrome virus (PRRSV) isolates (HB-1(sh)/2002, HB-2(sh)/2002 and JX-1/2002) were obtained from pig farms in Hebei and Jiangxi provinces, China. The complete ORF5 gene of the isolates was amplified using RT-PCR and sequenced. It was shown that ORF5 genes of all isolates encoded 200 amino acids. Comparing ORF5 genes of the three isolates and published sequences for five other PRRSV isolates in China, variation analysis showed that all of the isolates were of the American genotype, with 88.2–99.0% amino acid identity. ORF5 genes among BJ-4, S1 and J1 had higher similarity, sharing 98–99% identity of the deduced amino acids. HB-1(sh)/2002, HB-2(sh)/2002 and JX-1/2002 and CH-1a presented 92–96% identity among their ORF5 genes. Phylogenetic analysis revealed that these isolates could be divided into two subgroups based on the genetic distance of their ORF5 gene: the first subgroup comprised BJ-4, S1 and J1 and was closer to VR2332 and vaccine strains; the second included HB-1(sh)/2002, HB-2(sh)/2002, JX-1/2002 and CH-1a.

Keywords

Porcine reproductive and respiratory syndrome virusORF5 genevariation analysis
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 3 , December 2004 , pp. 173 - 179
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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